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There is an increased interest in the evolution and development of newts from the genus Triturus because: (1) morphological differentiation among the nine constituent species largely corresponds to different ecological preferences, (2) hybridization between different species pairs has various evolutionary outcomes in terms of life history traits and morphology, and (3) the genus expresses a balanced lethal system that causes arrested growth and death of half of the embryos. These features provide natural experimental settings for molecular, morphological, and life‐history studies. Therefore, we produce a staging table for the Balkan crested newt (T. ivanbureschi). We provide detailed descriptions of 34 embryonic stages based on easily observable and interpretable external morphological characters, to ensure reproducibility. Compared with previous staging tables for Triturus, we include a vastly increased sample size and provide high‐resolution photographs in lateral, ventral, and dorsal view, complemented by videos of specific developmental periods, and accompanied by detailed explanations on how to delineate the specific stages. Our staging table will serve as a baseline in comparative studies on Triturus newts: an emerging model system in evolutionary and developmental studies. Research highlights: We present a staging table of embryonic development of an emerging model system: the crested newt. Detailed descriptions of external morphology, complemented by photos and videos, make this a valuable resource for evolutionary developmental studies. [ABSTRACT FROM AUTHOR]

In this work, the Hypselotriton populations distributed in eastern Guangdong, China are studied in detail to clarify their taxonomic status. Based on morphological comparison and phylogenetic analysis, H. glaucus syn. nov. is synonymised with H. orphicus. Hypselotriton (Cynotriton) oolong sp. nov. from Mt Fenghuang in Chaozhou which used to be misidentified as H. orphicus, is revealed to be an independent lineage of subgenus Cynotriton and can be distinguished from all known congeners in morphology. By contrast, H. orphicus did not cluster within Cynotriton, but gathered with H. jiaoren comb. nov. to form a distinct unnamed clade within the genus. We therefore re-delimitate the intrageneric classification of the genus and a new subgenus Hakkatriton subgen. nov. is erected, corresponding to this unnamed clade. The Chinese Fire-bellied Newt genus Hypselotriton currently contains three subgenera and about ten known species. Identified keys to the subgenera and related congeners of genus Hypselotriton are further provided. [ABSTRACT FROM AUTHOR]

Age and body size in a lowland population of Lissotriton schmidtleri in the İstanbul Province of Türkiye were examined by applying the skeletochronology method. The age range varies between 5 to 11 years in males and 4 to 8 years in females. The mean age and SVL values were 8.1 years and 36.28 mm in males and 6.4 years and 37.72 mm in females. The mean SVL and age values showed a significant difference between the sexes. The present study showed sexual dimorphism and different life expectancy between sexes. The estimated potential reproductive lifespan was higher in males than females. [ABSTRACT FROM AUTHOR]

Lyciasalamandra species, like most amphibians, secrete a wide array of compounds from their granular and mucous skin glands, including the internally synthesized samandarine alkaloids, making their skin a complex organ performing a variety of functions. Lyciasalamandra helverseni and L. luschani basoglui are insular endemics of the Dodecanese islands of SE Greece, bearing distinct isolated populations, with well-documented phylogenetic profiles. Here, we employ a metabolomics approach, utilizing UPLC–ESI-HRMS/MS data of the skin secretions sampled from a number of specimens found in the islands of Karpathos, Kasos and Kastellorizo, in an effort to reveal aspects of their chemistry and diversity across populations. The results indicated statistically significant variation between all taxa examined, based on various secreted compounds. The underlying factors of variation highlighted by the multivariate analysis were differences in samandarine and other alkaloid content as well as in animal size. Metabolite annotation, based on dereplication tools and most importantly HRMS and HRMS/MS spectra, yielded a number of known samandarine alkaloids, reported for the first time in the currently studied Lyciasalamandra species. We also present documentation for novel members of the samandarine alkaloid family, as well as preliminary evidence for a possible dietary alkaloid sequestration. This work can set the basis for further research of this often-neglected endemic species of the Salamandridae, as well as the structural investigation of the samandarine alkaloid group. [ABSTRACT FROM AUTHOR]

This study explores how climate variables influenced the evolution and diversification of Neurergus newts within the Irano‐Anatolian biodiversity hotspot. We use a dated phylogenetic tree and climatic niche models to analyze their evolutionary history and ecological preferences. Using genetic data from nuclear (KIAA) and mitochondrial (16s and 12s) genes, we estimate divergence times and identify four major Neurergus clades. The initial speciation event occurred approximately 11.3 million years ago, coinciding with the uplift of the Zagros and Anatolian mountains. This geological transformation isolated newt populations, likely triggering the first speciation event. By integrating potential geographic distribution with climate variables, we reconstruct ancestral niche occupancy profiles. This highlights the critical roles of temperature and precipitation in shaping Neurergus habitat preferences and distribution. We observe both phylogenetic niche conservatism and divergence, with niche divergence playing a dominant role in diversification. This research emphasizes the complex interplay of geography, climate, and ecology in speciation and the vulnerability of isolated mountain newt populations to environmental changes. [ABSTRACT FROM AUTHOR]

The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between‐limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero‐posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs. [ABSTRACT FROM AUTHOR]

The trophic niche is one of the most important ecological traits for any species, providing information about trophic position in the food web, habitat preferences, and interspecific interactions. In this study, we describe the autumn diet of two sympatric species of salamander from central Italy—Italian Cave Salamanders (Speleomantes italicus) and Fire Salamanders (Salamandra salamandra), assessing whether competition for the same prey occurs. Furthermore, we combine our data with those obtained for the same population during a different season (spring) and assess potential seasonal variation in the diet of these two salamander species. The overlap of the trophic niche between the two species was limited, with S. italicus consuming smaller and fast-moving prey (e.g., Diptera, Collembola), and S. salamandra consuming slow and often elongated prey (e.g., Gastropoda, Haplotaxida, Diplopoda). Seasonal differences in diet composition were observed in both species between spring and autumn; in autumn, both species narrowed their trophic niche breadth. This seasonal divergence is likely due to the variation of prey phenology and availability. The consumption of prey of different size was likely the main factor allowing the coexistence of these two salamander species. In both species we observed a general increase in the proportion of generalist individuals in autumn, most likely because of a reduction in prey availability. In addition to providing information on the trophic niche of these species, our study represented a further step that helps unravel the dynamics promoting the coexistence of potentially competing species. [ABSTRACT FROM AUTHOR]

Figures 1, 2, and 4. The species name "N. derjugini" was incorrectly written as "N. microspilotus."The following figures have been updated accordingly:..FIGURE 2.FIGURE 4We apologize for this error. [Extracted from the article]

Traditional methods of phylogenetic reconstruction and species delimitation may be impeded by frequent hybridization among lineages. In this study, we conducted phylogenetic and clustering analyses of ddRAD genomic data on the entire genus Salamandra, which includes six species and over 25 subspecies of terrestrial salamanders. We expanded previous datasets to include missing subspecies and incorporated new samples, with an emphasis on secondary contact zones. Results obtained from a full dataset of 392 individuals (356,874 bp; 24,192 SNPs) were compared with those obtained after excluding substantially admixed individuals (n = 95; 835,467 bp; 51,557 SNPs) to explore the consequences of introgression on phylogenetic inference and taxonomic arrangement of subspecies. We found conflicting phylogenetic placements for taxa represented by many admixed individuals (identified by clustering ancestries). In contrast, a time-calibrated tree constructed without hybrids largely agrees with previous phylogenetic hypotheses. Within S. atra, we found paraphyly of S. atra atra, suggesting an additional candidate subspecies. Within S. infraimmaculata, two lineages are assignable to known subspecies and we additionally identified a third, deeply diverged lineage sampled near the Turkish/Syrian border. In S. algira, we found limited admixture between the subspecies S. a. tingitana and S. a. splendens despite their geographic proximity. Finally, within S. salamandra, we detected significant levels of hybridization between subspecies, which blurred their phylogenetic relationships, although the removal of admixed samples in subset analyses clarified the situation in most cases. Monophyly was recovered for subspecies that were previously found paraphyletic, including S. s. salamandra, S. s. gallaica, and S. s. fastuosa. Salamandra s. "alfredschmidti" was confirmed to be a junior synonym of S. s. bernardezi. Previously disputed subspecies, like S. s. "molleri" and S. s. "hispanica", correspond to separated lineages but are affected by admixture with other lineages. Further newly identified candidate subspecies in S. salamandra included a southern lineage within S. s. wer-neri and a western lineage within S. s. bernardezi. Finally, we re-evaluate the status of recognized subspecies in Salamandra, based on evidence from multiple delimitation criteria. Given that the evolutionary history could not be resolved for all subspecies, we highlight taxa within Salamandra that warrant further molecular examination and taxonomic revision, notably within the S. s. gallaica/"molleri"/bejarae complex. This study illustrates the impact of hybridization in phylogenetic analyses and its downstream effects in the identification of conservation units and their naming in the Linnean classification. [ABSTRACT FROM AUTHOR]

We analyzed data from 3,546 occurrence records of Fire Salamanders (Salamandra salamandra) from the territory of the Czech Republic where it has a heterogeneous distribution pattern. Its occurrence in terrestrial habitats adjacent to breeding streams is characterized by a mean distance of 103 m, maximum 1,321 m, from the nearest stream. Based on a logistic GLM (generalized linear model) analysis we determined the Fire Salamanders' climatic and habitat associations at landscape scale in the Czech Republic. The main limiting factors for its dispersal include the proximity of small shallow streams in rolling landscapes, the pH of these streams, the presence of broad-leaf or mixed forests, humidity, solar irradiation, and severity of winters. While the absence of suitable breeding habitats is the main limiting factor for their occurrence in more expansive lowlands, factors such as lower temperatures, lower humidity, and the absence of broad-leaf and mixed forests begin to play a role with increasing altitude. Unlike cold temperatures, low amounts of precipitation and higher temperatures do not act as limiters in the Czech Republic. Fire Salamanders respond to the colder climate of higher altitudes with intensified summer activity and the purely bimodal nature of their activity (with spring and autumn peaks) tends to fade at higher altitudes. [ABSTRACT FROM AUTHOR]

A new newt species, Hypselotriton huanggangensis sp. nov., is described based on nine specimens collected from Huanggangshan Mountains, Yanshan County, Jiangxi, China. Morphologically, the new species is characterized by the combination of nine external characters: (1) obvious black patches with clear boundaries on the whole body; (2) ground color of the dorsal body tan; (3) ground color of venter bright orange; (4) skin rough; (5) vertebral ridge weak; (6) fingers and toes overlapping when forelimb and hindlimb adpressed towards each other along body; (7) postocular orange spot absent; (8) small white warty glands around the eye; (9) two discontinuous longitudinal lines formed by white warty glands from neck to lateral parts of tail. Molecularly, the new species forms an independent clade with strong support in the phylogenetic trees of the genus based on the mitochondrial locus of NADH dehydrogenase subunit 2 (ND2) gene fragments. The new species distinctly differs from H. fudingensis by differences in its body measurements, vertebral ridge, dorsal black patches, and ventral black patches. Furthermore, the new species and H. fudingensis are geographically isolated by a series of high mountain ranges, including the Wuyishan and Jiufengshan Mountains. The number of Hypselotriton species is now 11. [ABSTRACT FROM AUTHOR]

Iberian populations of large-bodied newts, with Triturus marmoratus in the north and T. pygmaeus in the south of the peninsula, were studied for external morphology, mitochondrial dna and for a panel of single nucleotide polymorphisms. This confirmed the species' low level of interspecific hybridization and their parapatric, mosaic-like mutual range border across the peninsula. The genetic data also revealed substantial variation within T. pygmaeus, with narrow (0.43-35.2 km) clinal transitions in the very centre of Portugal. Similar clines were observed for body size and colouration pattern. Pygmy newts in the west of Portugal are larger, with a more striped (less reticulated) green dorso-lateral colouration pattern than those in the east and south of the country. The western group of populations is described as a new species, Triturus rudolfi sp. nov., on account of a long, ca. 2.5 Ma, independent evolutionary history and limited hybridization with its sister-species T. pygmaeus, suggesting selection against hybrid offspring. The range of the newly described species may be restricted to the wider Lisbon Peninsula, stretching northwards along the Atlantic coast to the river Vouga estuary. Inland, the range border may be set by the lower Tejo River, or by the currently wide area of agricultural land at either side of that river, that may accommodate a residual hybrid zone. The close contact between both pygmy newt species is effectively limited to a ca. 20 × 40 km area directly north of the town Entroncamento, where T. rudolfi sp. nov. is sandwiched in between T. marmoratus and the river Tejo. [ABSTRACT FROM AUTHOR]

Morphological and genetic variation of organisms is generally lower in northern than in southern regions of the Palearctic. This ubiquitous geographical pattern has been associated with extinction and recolonization processes in the north versus persistence in the Mediterranean area, as governed by climate change in the Pleistocene. One area of differentiation and complexity is the Iberian Peninsula where two distinct sister-species of marbled newts possess adjoining and geographically sharply delimited ranges over the north (Triturus marmoratus) and the south of the peninsula (T. pygmaeus). Within the latter species two forms are here documented, with different colouration patterns and non-overlapping ranges. The southern form is striped, much as in T. marmoratus, and occurs to the south of the Guadalquivir River. The northern form, here described as a new subspecies, has a reticulated dorsal colouration. It occurs only north of the Guadalquivir and is thus sandwiched in between two striped marbled newt taxa. Both T. pygmaeus subspecies engage inside the Doñana National Park in a ca. 2,500 m narrow contact zone. Although an inferred substantial selection against hybrids goes a long way to support species status, a reanalysis of published genetic data is required to solve this issue, along with the wider investigation of the same type of (microsatelite) data for the southern, nominotypical subspecies. [ABSTRACT FROM AUTHOR]

Introduction: Given the role of microbiomes in promoting host health and homeostasis, understanding the factors shaping skin microbial communities in wild vertebrates has become increasingly important in conservation. This goal is even more pressing for amphibians, for which the skin has multiple critical functions, and pathogens currently decimating populations are linked to significant changes in skin microbiomes. However, because microbiomes are also shaped by environmental and ecological influences, as well as by host phylogeny, it is important to quantify these contributions to microbiome structure in the presence of infection. Methods: To understand the joint influence of these diverse factors shaping microbiomes, we used 16S rRNA sequencing to characterize the skin microbial communities of six salamander species (families Plethodontidae and Salamandridae) found in Oklahoma and contrasted the effects of infection status, phylogeny, host ecology, and host environment (i.e., climate) on skin microbiomes. Results: Differences at the level of host family were the main factor influencing microbiome diversity; however, we did not detect a substantial phylogenetic signal. Instead, host ecology and environment were more important in driving microbiome differences among species and genera. Salamanders that tested positive for the skin fungal parasite Batrachochytrium dendrobatidis (Bd) also had slightly less diverse microbiomes than Bd-free animals, but no such differences were associated with the systemic pathogen ranavirus (RV). Discussion: Together, these results indicate a nuanced relationship between the number and type of microbes present on salamander skin and the factors influencing them. By developing a baseline assessment of the microbiome diversity and richness present on the skin of these focal species, this work also provides a foundation for monitoring and evaluating changes in skin microbiomes as populations continue to experience stressors and diseases. [ABSTRACT FROM AUTHOR]

Abstract This study explores how climate variables influenced the evolution and diversification of Neurergus newts within the Irano‐Anatolian biodiversity hotspot. We use a dated phylogenetic tree and climatic niche models to analyze their evolutionary history and ecological preferences. Using genetic data from nuclear (KIAA) and mitochondrial (16s and 12s) genes, we estimate divergence times and identify four major Neurergus clades. The initial speciation event occurred approximately 11.3 million years ago, coinciding with the uplift of the Zagros and Anatolian mountains. This geological transformation isolated newt populations, likely triggering the first speciation event. By integrating potential geographic distribution with climate variables, we reconstruct ancestral niche occupancy profiles. This highlights the critical roles of temperature and precipitation in shaping Neurergus habitat preferences and distribution. We observe both phylogenetic niche conservatism and divergence, with niche divergence playing a dominant role in diversification. This research emphasizes the complex interplay of geography, climate, and ecology in speciation and the vulnerability of isolated mountain newt populations to environmental changes.

Seasonal activity of the Japanese red-bellied newt, Cynops pyrrhogaster, was monitored in Minami-ohsawa, Hachioji-shi, Tokyo, from 2016 to 2017. The number of newts in the water of a pond was counted immediately after sunset, at least once a week, except in winter. The number of newts counted varied daily, and the time-series data on newt activity were analyzed using state-space modeling to estimate the impact of weather conditions and lunar phase on the activity due to the presence of plausible temporal autocorrelation. We found that some weather conditions, such as water temperature and atmospheric pressure, had significant positive effects on newt activity. Newts became more active during nights with higher temperatures and/or higher pressure. However, we did not detect any significant effect of the lunar phase on newt activity. The most important variable affecting newt activity was water temperature. The active season of newts was estimated to be nine months from March to November. Newt activity showed a sharp decline in the nightly activity of males and an extreme female-biased sex ratio was observed in early April suggesting that the degree of nocturnality differed across seasons and the two genders. This phenomenon may be related to the presence of mating activity during this particular season. [ABSTRACT FROM AUTHOR]

The analysis of the parasitic and endosymbiontofauna of the common newt (Lissotriton vulgaris L., 1758) in the natural and urban landscapes of the Middle Urals is presented for the first time. Four species of helminths belonging to the Nematoda type were found, including one taxon indeterminate to the species (Nematoda sp.) and one species of endosymbionts (phylum Chromista). The works devoted to the species composition of L. vulgaris parasites on the territory of Eurasia are analyzed. The fauna of the helminths of the common newt on the eastern slope of the Urals is depleted, which is probably determined by the limited distribution of the species outside Europe, the temperature regime in the north, and the availability of moisture in the south. [ABSTRACT FROM AUTHOR]

The evolutionary causes behind the development of taste discs (TDs), recognized as the only gustatory organs in metamorphosed Batrachia (Caudata and Anura), have yet to be determined. Taste buds (TBs) serve as the gustatory organs in aquatic batrachian larvae, as well as in both larval and adult caecilians (Gymnophiona) inhabiting either aquatic environments or moist terrestrial habitats. We describe the morphology of gustatory organs of adult crocodile newts (Tylototriton cf. verrucosus , Salamandridae, Caudata). Crocodile newts are semi-aquatic and exhibit increased specialization towards an aquatic feeding, distinguishing it from most members of the subfamily Pleurodelinae. Our findings confirm that TDs serve as the gustatory organs in adult T. cf. verrucosus , similarly to many batrachians. We also determined the density and sizes of TDs' sensory zones on the tongue and palate of T. cf. verrucosus , which differs from Batrachia. In terms of micro-morphology, two types of gustatory cells (GCs) were identified in the gustatory epithelium of T. cf. verrucosus. Our literature analysis suggests that TDs were larger in anurans compared to caudate amphibians, but were also larger in aquatic/semi-aquatic than in terrestrial anurans. [ABSTRACT FROM AUTHOR]

Updated information on the distribution and studied populations of T. cristatus on the eastern macro-slope of the Urals (Middle and Southern Urals) is provided. Based on tagging with recapture, the number of reproductive individuals of the populations, the sex ratio, and the morphological specificity are estimated for the first time. From the standpoint of assessing the physicochemical features of the habitat environment, possible limiting factors (thermal and moisture availability, geochemical characteristics, climate continentality, and the presence of natural enemies and invasive species) that affect the distribution and abundance of the great crested newt near the northeastern limit of the range are discussed. [ABSTRACT FROM AUTHOR]

One of the main factors creating intraspecific morphological variation, sexual dimorphism (SD) could be expressed as adult male and female animals having different body sizes or shapes. Female-biased sexual dimorphism has been documented in the vast majority of amphibians and more than half of salamanders. In this study, 18 morphometric characters were used to analyze the size and shape dimorphism of the southern banded newt, Ommatotriton vittatus, a species whose congeners exhibit male-biased dimorphism. In this way, the hypothesis that species within the same genus would have similar sexual dimorphism (for example, male- or female-biased) was tested. Results of the current study confirmed the existence of male-biased sexual size and shape dimorphism in O. vittatus. For instance, snout-vent length and tail length were found to be significantly different between sexes, with males being larger. Moreover, males have larger forelimbs and hindlimbs than females. Data from the present study also indicated significant male-biased differences in five (head length and width, eye diameter, distance between the orbit and naris, and internarial distance) out of eight head characters. This result supports the assumption that species within the same genus will have a similar tendency for sexual dimorphism. [ABSTRACT FROM AUTHOR]

Species with wide‐range distributions usually display high genetic variation. This variation can be partly explained by historical lineages that were temporally isolated from each other and are back into secondary reproductive contact, and partly by local adaptations. The smooth newt (Lissotriton vulgaris) is one of the most widely distributed amphibians species across Eurasia and forms a species complex with a partially overlapping distribution and morphology. In the present study, we explored the population genomic structure of smooth newt lineages in the Carpathian Basin (CB) relying on single‐nucleotide polymorphisms. Our dataset included new and previously published data to study the secondary contact zone between lineages in the CB and also tested for the barrier effect of rivers to gene flow between these lineages. We confirmed the presence of the South L. v. vulgaris Lineage distributed in Transdanubia and we provided new distribution records of L. v. ampelensis inhabiting the eastern territories of the CB. High genetic diversity of smooth newts was observed, especially in the North Hungarian Mountains and at the interfluves of the main rivers in the South with four distinct lineages of L. v. vulgaris and one lineage of L. v. ampelensis showing a low level of admixture with the spatially closest L. v. vulgaris lineage. Moreover, admixture detected at the interfluve of the main rivers (i.e. Danube and Tisza) suggested a secondary contact zone in the area. Finally, we found that the river Danube has a very weak effect on population divergence, while the river Tisza is a geographical barrier limiting gene flow between smooth newt lineages. As the range boundaries of L. v. vulgaris and L. v. ampelensis in the CB coincide with the river Tisza, our study underpins the influence of rivers in lineage diversification. [ABSTRACT FROM AUTHOR]

Serially homologous structures may have complex patterns of regionalization and morphological integration, influenced by developmental Hox gene expression and functional constraints. The vertebral column, consisting of a number of repeated, developmentally constrained, and highly integrated units—vertebrae—is such a complex serially homologous structure. Functional diversification increases regionalization and modularity of the vertebral column, particularly in mammals. For salamanders, three concepts of regionalization of the vertebral column have been proposed, recognizing one, two, or three presacral regions. Using three‐dimensional geometric morphometrics on vertebra models acquired with microcomputerized tomography scanning, we explored the covariation of vertebrae in four closely related taxa of small‐bodied newts in the genus Lissotriton. The data were analyzed by segmented linear regression to explore patterns of vertebral regionalization and by a two‐block partial least squares method to test for morphological integration. All taxa show a morphological shift posterior to the fifth trunk vertebra, which corresponds to the two‐region concept. However, morphological integration is found to be strongest in the mid‐trunk. Taken jointly, these results indicate a highly integrated presacral vertebral column with a subtle two‐region differentiation. The results are discussed in relation to specific functional requirements, developmental and phylogenetic constraints, and specific requirements posed by a biphasic life cycle and different locomotor modes (swimming vs. walking). Further research should be conducted on different ontogenetic stages and closely related but ecologically differentiated species. Research Highlights: •The studied taxa of Lissotriton newts show a common pattern of regionalization, with a morphological transition after the fifth trunk vertebra.•The vertebral column is morphologically integrated, most strongly in the center of the trunk region.•Taken jointly, these results indicate a highly integrated presacral vertebral column with a subtle two‐region differentiation. [ABSTRACT FROM AUTHOR]

The Anatolian banded newt is an endemic amphibian species to the western and northern parts of Türkiye. The present study provides the new locality records of Ommatotriton nesterovi, which constitutes the species' easternmost known locality. With the record of the current study, the known distribution area of the species has been extended about 70 km eastward. Moreover, morphological comparisons were made between individuals from these two new localities and individuals from other regions. [ABSTRACT FROM AUTHOR]

Abstract Species with wide‐range distributions usually display high genetic variation. This variation can be partly explained by historical lineages that were temporally isolated from each other and are back into secondary reproductive contact, and partly by local adaptations. The smooth newt (Lissotriton vulgaris) is one of the most widely distributed amphibians species across Eurasia and forms a species complex with a partially overlapping distribution and morphology. In the present study, we explored the population genomic structure of smooth newt lineages in the Carpathian Basin (CB) relying on single‐nucleotide polymorphisms. Our dataset included new and previously published data to study the secondary contact zone between lineages in the CB and also tested for the barrier effect of rivers to gene flow between these lineages. We confirmed the presence of the South L. v. vulgaris Lineage distributed in Transdanubia and we provided new distribution records of L. v. ampelensis inhabiting the eastern territories of the CB. High genetic diversity of smooth newts was observed, especially in the North Hungarian Mountains and at the interfluves of the main rivers in the South with four distinct lineages of L. v. vulgaris and one lineage of L. v. ampelensis showing a low level of admixture with the spatially closest L. v. vulgaris lineage. Moreover, admixture detected at the interfluve of the main rivers (i.e. Danube and Tisza) suggested a secondary contact zone in the area. Finally, we found that the river Danube has a very weak effect on population divergence, while the river Tisza is a geographical barrier limiting gene flow between smooth newt lineages. As the range boundaries of L. v. vulgaris and L. v. ampelensis in the CB coincide with the river Tisza, our study underpins the influence of rivers in lineage diversification.

The Japanese fire-belly newt, Cynops pyrrhogaster, is a near threatened species that is conservation dependent. Here, we examine feeding habits across the year within a genetically divergent intraspecific lineages of this species (the Central Lineage) to provide information to support future insitu and ex-situ conservation activities. Stomach contents from newts were collected in a paddy field habitat, Kyoto City, central Honshu, Japan for two consecutive years. Throughout the year, dipteran aquatic larvae were the most important food source both for males and females, although terrestrial invertebrates were also important prey for the newts. During periods of low prey availability shed skin could also be a relatively valuable source of food. Sympatric frogs and newts appear to target different prey and this may facilitate their co-existence. Our study provides new information on suitable prey items for the Central Lineage of C. pyrrhogaster both in its natural habitat and in captivity. [ABSTRACT FROM AUTHOR]

Studying the relationships between predators and their prey is generally complex but provides valuable knowledge into the process of evolution. The clay model method is a technique that has been widely used to assess prey-predator interaction. In the study, we used clay models of the Vietnam warty newt (Paramesotriton deloustali) to evaluate its predator pressures in Tam Dao National Park (NP), northern Vietnam. We also employed camera traps to detect specific predators of the newt in nature. Our camera trap results showed that northern treeshrew (Tupaia belangeri), rats (Rattus sp.), and greater coucal (Centropus sinensis) are predators of the newt in Tam Dao NP. For the clay model experiment observed attacks on the head of clay models were triple those expected by chance, indicating that predators perceived the clay model as actual prey items. The proportions on the models predated upon differed in three habitat types: broadleaf evergreen forests, mixed broadleaf evergreen and bamboo forests, and bamboo forests. We also detected that the attacks on the models were mainly made by mammals. Attack rates at nighttime were three times higher than during the daytime. [ABSTRACT FROM AUTHOR]

Amphibians are small ectothermic vertebrates with high permeable skins that are highly constrained by environmental conditions, such as humidity and temperature. Therefore, terrestrial salamanders regulate their surface activity, including foraging, to minimize evaporative water loss. The influence of local micro-climatic factors on the foraging success of European forest dwelling salamanders has rarely been analysed in detail. We examined the role of local climatic conditions on the foraging success, measured by the total number of prey items, of two syntopic forest-dwelling salamanders: Strinati's cave salamander, Speleomantes strinatii (family Plethodontidae), and Northern spectacled salamander, Salamandrina perspicillata (family Salamandridae). We evaluated the effects of rainfall, temperature and body size on the foraging success of these two species, in eight sampling sites in the Northern Apennines of Italy, for a total of 11 salamander populations. Our findings show that different climatic variables affected the two species differently. Salamandrina perspicillata foraged more intensively during periods of prolonged rainfall with higher temperatures. On the contrary, S. strinatii foraging success was negatively influenced by rising temperature, while rainfall seemed to have no significant effect. Interestingly, body size increased success in S. perspicillata but not in S. strinatii. Overall, our findings show that, when foraging in the same environment, these two species are conditioned by different climatic factors possibly due to their differences in overall body size and their ability to resist dehydration. [ABSTRACT FROM AUTHOR]

A new species of the genus Tylototriton is described from Ngoc Linh Mountain, Kon Tum Province, in the Central Highlands of Vietnam based on integrative taxonomy, namely by combining molecular and morphological evidence. Tylototriton ngoclinhensis sp. nov. differs from all other congeners based on morphological data, allopatric distribution, and molecular divergence. In terms of genetic divergence, Tylototriton ngoclinhensis sp. nov. distinctly differs from the sister species T. panhai (6.77%) and from T. ngarsuensis (12.36%) based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) gene. Tylototriton ngoclinhensis sp. nov. is a moderate sized and robust salamander species with large cephalic edges, parotoids, and vertebral ridge orange in coloration. The new taxon differs from its congeners by a combination of the following morphological characteristics: size medium (SVL 60.8-66.5 mm, TL 57.6-61.8 mm in males, and SVL 72.5-75.6 mm, TL 62.9-67.9 mm in females); head longer than wide; parotoids very prominent and enlarged, projecting backwards; tail length shorter than snout-vent length; vertebral ridge large, high and glandular in appearance; 14 large and distinct dorsolateral glandular warts; gular fold present; tips of fore and hind limbs overlapping when adpressed along the body; tips of fingers reaching between eye and nostril when foreleg is laid forward; dorsal surface and lateral sides of the head, upper and lower lips, dorsolateral glandular warts, vertebral ridge, the peripheral area of the cloaca and the ventral edge of the tail orange in coloration; the presence of a distinct black line extending from the posterior end of the eye towards the shoulder. Tylototriton ngoclinhensis sp. nov. is restricted to evergreen montane forests near water bodies on Ngoc Linh Mountain. We suggest that the new species should be classified as Endangered (EN) in the IUCN Red List. This new important discovery represents the eighth Tylototriton taxon described from Vietnam, and at the same time constitutes the southernmost distributional record for the whole genus in Asia. [ABSTRACT FROM AUTHOR]

For at least two decades, European amphibians have been affected by the ‘salamander plague’, an emerging infectious disease caused by the invasive chytrid skin fungus Batrachochytrium salamandrivorans (Bsal) that originated from Asia. Resulting dramatic declines are well known from the European Fire Salamander, while the impact of Bsal on other European amphibians in the wild is less clear. We here focus on the Northern Crested Newt (Triturus cristatus) in Germany. This species is susceptible to Bsal and strictly protected under Annex II of the EC Habitats Directive. While T. cristatus is declining in the Bsal-hotspot Germany, it remains to be answered if the salamander plague is one of the leading drivers. By asking five key questions we review the available information with the goal of providing a baseline for further research. In two investigated newt communities, Bsal-prevalence was found to be considerably high, with 75–89% of newts being infected, reaching the highest peaks in spring. Later in the year, some T. cristatus were apparently able to clear their Bsal infection, even when individual infection load was previously high. Although we observed mortality of infected specimens and declines in populations with Bsal, declines are also seen outside known Bsal regions. Thus, it appears that the Bsal epidemic is not exclusively responsible for the species’ current status. With this, Bsal poses a yet poorly-understood threat to Northern Crested Newt survival, making further research on pathogen/host interactions and long-term survival indispensable to fulfil our legal responsibility of conserving this emblematic species. [ABSTRACT FROM AUTHOR]

In this study, we used genetic approaches to assess the conservation status of a protected amphibian species, the Macedonian crested newt, Triturus macedonicus, in Northern Pindos National Park (Epirus, Greece). Mitochondrial DNA sequences and multilocus genotypes of individuals from 38 breeding sites were used to infer their phylogenetic position and to detect and measure genetic variation patterns, population genetic structure, and levels of gene flow. The examined individuals fell within two major clades of the Macedonian crested newt phylogeny, being geographically separated by the Aoos River valley and Vikos Gorge. Both groups constitute separate gene pools, bearing private haplotypes and alleles, and the groups were found to be highly differentiated in both their mitochondrial and microsatellite markers. Thus, they meet all of the criteria needed to be characterized as evolutionary significant units (ESUs) that deserve a separate conservation status. Within each ESU, the particularly high inter-population differentiation and low migration rates imply a lack of connectivity between breeding sites and local isolation in favorable habitats. Our results can inform future programs and actions towards the conservation and management of the Macedonian crested newt in the area. [ABSTRACT FROM AUTHOR]

The osteology of European urodeles was studied extensively in the past, but comparative analyses of isolated bones are rare, despite being the most useful tool for the identification of fossil remains. The present work is focused on the most robust skeletal elements (therefore, common in the fossil record: otic–occipitum complexes and vertebrae) and provides taxonomically significant diagnostic characters at the genus level for European genera of urodeles, including some taxa whose osteology was poorly known (e.g. Euproctus and Calotriton). Characters subject to wide variability are identified, and their use is discouraged for diagnoses of extinct species. A selection of diagnostic characters is used herein to build an identification key for each studied skeletal element and for a phylogenetic analysis to understand whether these osteological elements can convey a genuine signal. The analysis under Bayesian inference resulted in a well-supported Salamandridae clade and Pleurodelinae tribe. Salamandrina was recovered as part of the Pleurodelinae, in agreement with previous morphological phylogenetic analyses, but in contrast to the conclusion of molecular studies. [ABSTRACT FROM AUTHOR]

The great crested newt Triturus cristatus is a widely distributed species across Europe and its southernmost range reaches northwestern Bulgaria. Triturus cristatus is comparatively well-studied in general, however, data about its distribution and habitat preferences in Bulgaria are very scarce. This study presents the results of an extensive field research (carried out in 2021) and a review of all available previous data for the species in Bulgaria. All known localities of T. cristatus in the country fall into a total of 22 UTM-squares (10 x 10 km), for 10 of which the species is newly discovered. The distribution of T. cristatus in Bulgaria is wider than previously thought and covers almost the entire north-western part of the country. The altitudinal range is relatively wide (40–1380 m a.s.l.) but most of the localities are situated below 500 m. In terms of habitat preferences, our results demonstrate that T. cristatus prefers water bodies of small area (below 1000 m² ), shallow depth (0.5–1 m), muddy bottom, without reed/bulrush and without fish. Regarding the terrestrial phase, it can only be stated that in the vicinity (400 m radius) of the water bodies in which the species was found, the predominant type of land cover is “Agricultural areas”, followed by “Forest and semi natural areas”, but the preferences of T. cristatus to specific terrestrial habitats in the Bulgarian part of distribution range remain unknown. [ABSTRACT FROM AUTHOR]

The Anderson's crocodile newt, Echinotriton andersoni, is an endangered species distributed in the islands of Central Ryukyus, Japan. This species is well known to show high intraspecific genetic variation among islands, but its morphological variation has not been explored. We examined the skull variation in this species including enigmatic specimens collected from Taiwan by using geometric morphometrics, and found sexual differences and geographic variation mainly between the Okinawa group (Okinawajima Island) and the Amami Group (Amamioshima and Tokunoshima Islands). We further found close resemblance between the Taiwanese specimens and the female specimens from Amamioshima Island. [ABSTRACT FROM AUTHOR]

Mitochondrial genomes (mitogenomes) are valuable resources in molecular and evolutionary studies, such as phylogeny and population genetics. The complete mitogenomes of two crocodile newts, Tylototriton broadoridgus and Tylototriton gaowangjienensis, were sequenced, assembled, and annotated for the first time using next-generation sequencing. The complete mitogenomes of T. broadoridgus and T. gaowangjienensis were 16,265 bp and 16,259 bp in lengths, which both composed of 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and 1 control region. The two mitogenomes had high A + T content with positive AT-skew and negative GC-skew patterns. The ratio of non-synonymous and synonymous substitutions showed that, relatively, the ATP8 gene evolved the fastest and COI evolved the slowest among the 13 PCGs. Phylogenetic trees from BI and ML analyses resulted in identical topologies, where the Tylototriton split into two groups corresponding to two subgenera. Both T. broadoridgus and T. gaowangjienensis sequenced here belonged to the subgenus Yaotriton, and these two species shared a tentative sister group relationship. The two mitogenomes reported in this study provided valuable data for future molecular and evolutionary studies of the genus Tylotoriton and other salamanders. [ABSTRACT FROM AUTHOR]

Simple Summary: Studies on species' trophic niches are essential to understand the characteristics of species' ecology and life traits, as well as to improve conservation strategies. In the absence of competitors, species realize their trophic niche including in their diet the most profitable food resources. In the presence of competitors, species modify their preferences to reduce competition and maintain the highest benefits at the same time. In this study, we assessed the trophic niche of two species of salamanders coexisting in a forested area of Italy and evaluated which might be the mechanisms that these two species adopted to reduce competition. We found that the Italian cave salamander (Speleomantes italicus) mostly consumed flying prey with a hard cuticle, while the fire salamander (Salamandra salamandra) preferred worm-like and soft-bodied prey. In conclusion, we hypothesize that in our case, the two species of salamanders did not have to change their prey preference in order to avoid competition, but divergences in metabolism and behavioral traits likely worked as natural deterrent. The trophic niche of a species is one of the fundamental traits of species biology. The ideal trophic niche of a species is realized in the absence of interspecific competition, targeting the most profitable and easy-to-handle food resources. However, when a competitor is present, species adopt different strategies to reduce competition and promote coexistence. In this study, we assessed the potential mechanisms that allow the coexistence of two generalist salamanders: the Italian cave salamander (Speleomantes italicus) and the fire salamander (Salamandra salamandra). We surveyed, in April 2021, a forested area of Emilia-Romagna (Italy) during rainy nights. Analyzing the stomach contents of the captured individuals, we obtained information on the trophic niche of these two sympatric populations. Comparing our results with those of previous studies, we found that the two species did not modify their trophic niche, but that alternative mechanisms allowed their coexistence. Specifically, different prey preferences and predator metabolisms were likely the major factors allowing reduced competition between these two generalist predators. [ABSTRACT FROM AUTHOR]

The Paramesotriton Chang, 1935 genus of Asian warty newts is the second most diverse genus in the family Salamandridae, currently containing 14 recognized species from northern Vietnam to southwest-central and southern China. Although species of this genus have been included in previous phylogenetic studies, the origin and interspecific relationships of the genus are still not fully resolved, especially at key nodes in the phylogeny. In this study, we sequenced mitochondrial genomes and 32 nuclear genes from 27 samples belonging to 14 species to reconstruct the interspecific phylogenetic relationships within Paramesotriton and explore its historical biogeography in southern China. Both Bayesian inference and maximum-likelihood analyses highly supported the monophyly of Paramesotriton and its two recognized species groups (P. caudopunctatus and P. chinensis groups) and further identified five hypothetical phylogenetic cryptic species. Biogeographic analyses indicated that Paramesotriton originated in southwestern China (Yunnan-Guizhou Plateau/South China) during the late Oligocene. The time of origin of Paramesotriton corresponded to the second uplift of the Himalayan/Qinghai-Xizang (Tibetan) Plateau (QTP), rapid lateral extrusion of Indochina, and formation of karst landscapes in southwestern China. Principal component analysis (PCA), independent sample t-tests, and niche differentiation using bioclimatic variables based on locations of occurrence suggested that Paramesotriton habitat conditions in the three current regions (West, South, and East) differ significantly, with different levels of climatic niche differentiation. Species distribution model (SDM) predictions indicated that the most suitable distribution areas for the P. caudopunctatus and P. chinensis species groups are western and southern/eastern areas of southern China. This study increases our knowledge of the taxonomy, biodiversity, origin, and suitable distribution areas of the genus Paramesotriton based on phylogenetic, biogeographic, and species distribution models. [ABSTRACT FROM AUTHOR]

Evolutionary biologists are always interested in deciphering the geographic context of diversification, therefore they introduced the concept of comparative phylogeography, which helps to identify common mechanisms that contribute to shared genetic structures among organisms from the same region. Here, we used multi-locus genetic data along with environmental data to investigate shared phylogeographic patterns among three Asianendemic newt genera, Cynops, Paramesotriton and Pachytriton, which occurred in montane/submontane streams or ponds in southern China. Our 222 samples from 78 localities covered the entire range of the three genera and represented the largest dataset of this group to date. We reconstructed matrilineal genealogies from two protein-coding, mitochondrial genes, and gene network from two nuclear genes. We also estimated divergence times of major cladogenetic events and used occurrence data to evaluate niche difference and similarity between lineages. Our results revealed a common basal split in all three genera that corresponds to the separation of two geographic terrains of southern China. Those ancient divergence occurred during middle to late Miocene and likely correlate with paleoclimatic fluctuations caused by the uplift of the Qinghai-Xizang (Tibet) Plateau (QTP). Particularly, the strengthening and weakening of Asian summer monsoons during the Miocene may have profoundly impacted southern China and led to repeatedly vicariance in those newts. However, despite differences in realized niches between lineages, there is no evidence for divergence of fundamental niches. Preservation of old newt matriline lineages in mountains of southern China suggests that the region acts as both museums and cradles of speciation. Based on those results, we advocate a multi-pronged protection strategy for newts in the three genera. [ABSTRACT FROM AUTHOR]

The origin and biogenesis of tetrodotoxin (TTX) is one of the most interesting and perplexing questions remaining for TTX researchers. Newts can possess extreme quantities of TTX and are one of the most well-studied of all TTX-bearing organisms, yet seemingly conflicting results between studies on closely related species continues to generate debate. In this study, eggs from 12 female newts (Taricha granulosa) were reared in captivity and the metamorphosed juveniles were fed a TTX-free diet for 3 years. Using a non-lethal sampling technique, we collected skin samples from each individual each year. Wild-caught juveniles from the same population were also sampled for TTX. In lab-reared juveniles, mass increased rapidly, and after only 2 years individuals approached adult body mass. TTX levels increased slowly during the first two years and then jumped considerably in year three when fed a diet free of TTX. However, wild-caught juvenile newts of unknown age were more toxic than their lab-reared counterparts. These results, coupled with additional data on the long-term production and synthesis of TTX in adult newts suggest that TTX is unlikely to come through dietary acquisition, but rather newts may be able to synthesize their own toxin or acquire it from symbiotic bacteria. [Display omitted] • Rough-skinned newts reared from eggs for 3 years increased in TTX over this time. • Wild-caught juvenile newts had greater TTX levels relative to lab-reared newts. • TTX production in newts is not likely a result of dietary sequestration. • TTX can be interpreted to be derived endogenously and via symbiotic bacteria. • Lab vs. wild toxicity may be due to a trade-off between growth & toxin production. [ABSTRACT FROM AUTHOR]

The location, shape and area of dark spots on the belly, throat and sides of the body of three closely related species of smooth newts (Lissotriton kosswigi, L. schmidtleri and L. vulgaris) were analysed. Differences were found between species and sexes in the location and shape of dark spots on the belly, throat and lateral surfaces of the body. Discriminant analysis of these spot characteristics allows to identificate of males of all three species with a high degree of confidence (85--91%). In females, only L. vulgaris was correctly distinguished from the other two species (accuracy 81--94%). Anatolian and Thracian populations of L. schmidtleri have very similar patterns of dark spots, which confirm their conspecificity. The differences in the location and size of dark spots make possible successful identification of the species during field research and study of museum specimens. The developed method could be useful for comparative studies of other animal species which have spotted camouflage or aposematic colouration. [ABSTRACT FROM AUTHOR]

Developmental plasticity, a common pattern in lissamphibian evolution, results in numerous alternative morphologies among species and also within populations. In the present study, a natural population of the salamander Taricha granulosa (Salamandridae) was examined to detect variation in the vertebral count and to identify potential deformities of their vertebral column. The number of trunk vertebrae varied between 11 and 13 and we recorded 58 individuals with 69 anomalous vertebral elements. These anomalies range from congenital malformations (block vertebrae, unilateral bars, hemivertebrae), extra ossifications in the haemal region, to posttraumatic pathologies. Most osseous pathologies were encountered in the caudal region of the axial skeleton. Our data suggest a high frequency of vertebral malformations in salamanders; however, the identification of the exact causes remains challenging. [ABSTRACT FROM AUTHOR]

The fire salamander (Salamandra salamandra) is a cold-tolerant amphibian species. Recent data proved that it feeds intensely at the beginning of spring at low temperatures, and we hypothesised that the same is also true for the autumn. To verify this, we analysed the feeding of a population of S. salamandra from Iron Gates Natural Park during three different days from mid-October to mid-November 2020. During the study period, S. salamandra fed normally, as the number of prey items, prey taxa and food diversity were high. The feeding intensity did not decrease constantly toward winter but fluctuated. The most common preys were of Limacidae and Diplopoda. Most prey categories were flightless and characteristic for humid areas and forests, including some endemic terrestrial isopods distributed only in southwestern Romania (Armadillidium banaticum). This fact highlights the importance of such habitats for the survival of both the fire salamander and its prey. [ABSTRACT FROM AUTHOR]

Skeletal development in salamanders is greatly influenced by their complex life history. Although the relationship between skull development and life history has been investigated in a variety of salamander species, few studies have compared skull development among lineages. Here, we describe the cranial skeletogenesis of the Japanese giant salamander, Andrias japonicus (family Cryptobranchidae), for the first time. To conduct interspecific comparisons, we also describe the cranial skeletogenesis of Hynobius nebulosus (Hynobiidae), Pleurodeles waltl (Salamandridae) and Ambystoma mexicanum (Ambystomatidae). Our comparative analyses reveal interspecific differences in cranial skeletogenesis, particularly in the timing of the onset of ossification, the direction of ossification (e.g. from anterior to posterior) and the ossification pattern of the hyobranchial skeleton. The early onset of jaw ossification, posterior extension of the maxilla and posterolateral inclination of the squamosal in An. japonicus might be adaptations that allow An. japonicus to gulp feed, primarily using the suction force generated by opening the mouth wide, while also supplementing that force by depressing the hyobranchial skeleton. Multivariate regression of skull shape on log10-transformed centroid size revealed that the unique skull shape of adult and subadult An. japonicus forms through hypermorphosis. [ABSTRACT FROM AUTHOR]

Background West Siberia is a large region in North Eurasia, which harbours multiple climatic zones, landscape types and biomes. Its amphibian fauna is characterised by a combination of European and Asian species. For many species, this region is the place where the limits of their global ranges are located (Rana temporaria, R. amurensis, Bufotes sitibundus). West Siberia also has at least two non-native amphibian species (Pelophylax ridibundus, Bufotes viridis). The exact ranges and patterns of distribution of the West Siberian amphibian species are poorly studied. The mapping of species ranges is important for the development of conservation measures and monitoring of invasive species is required to investigate their impacts on the natural ecosystems. New information This work presents the most complete biogeographic and occurrence records database of the amphibians of West Siberia. To assemble the database, we digitised data from 190 published works, obtained data from major museum collections and from the data bank on the abundance and distribution of animals «Zoomonitor» by the Zoomonitoring laboratory of the Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences. The database also includes original and partly unpublished data collected by the authors from 1975 to 2021, as well as quality-assessed citizen science data from the iNaturalist portal. In total, the database contains 2530 records for 11 species of amphibians, including the locality data, the observation date (when known) and the source of the observation (at least one of the following: literature reference, museum sample ID, observer’s name, iNaturalist link). [ABSTRACT FROM AUTHOR]

Calotriton arnoldi is an endemic amphibian inhabiting Montseny Natural Park and Biosphere Reserve (PNRBM), listed as "critically endangered (CR)" by IUCN. At the end of 2016, the Life Tritó del Montseny (LIFETM) project (LIFE15 NAT/ES/000757) was launched. The aim of the project was to promote around fifty actions to ensure the conservation of C. arnoldi and its natural habitat, and this entailed five strategic lines: (1) Increasing the scientific and technical knowledge with regard to C. arnoldi's conservation status and its habitat management. (2) Expanding its geographic distribution. (3) Involving and engaging stakeholders in the conservation of the Montseny brook newt. (4) Eliminating or minimizing threats that exist in the riparian habitat. (5) Establishing proper legal coverage and defining long-term strategic planning. The successes and failures experienced throughout the process provide us with essential information that will enable us to develop an adaptive management of the habitat. In order to eliminate or minimize threats to the newt's habitat, some of the actions that are currently being carried out are: (a) Land acquisitions and land exchanges with private properties. (b) Land stewardship procedures, with two custody agreements being signed. (c) Reduction of water withdrawal with nine water catchments and distribution being remodeled. (d) Improvement of water treatments and storage by installing ecological wastewater treatment facilities. (e) Ensuring ecological connectivity and riparian forest restoration. Here, we present an evaluation of the actions carried out to improve the habitat of this species, including the necessary considerations for them to be implemented correctly and to be successful in a natural area, which is under public-private management. [ABSTRACT FROM AUTHOR]

Rough-skinned newts, Taricha granulosa , which contain tetrodotoxin (TTX), a potent neurotoxin, are widely distributed along the west-coast of North America up to British Columbia (BC), Canada, and Southeast Alaska. Their genetic population structure using DNA-microsatellites and the TTX-content of specimens from British Columbia (Prince Rupert area) and Alaska (Revillagigedo Island, Shelter Island, and Juneau) were analysed. TTX-concentrations were low in newts from BC and Revillagigedo Island, but high in specimens from mainland Juneau, which had been deliberately introduced from Shelter Island, where TTX was not detectable in the individuals sampled. No significant genetic differences were detected between these populations, which may correlate with the high intraspecies variability of TTX. It is still an open question, which factors favour or induce the toxin production in the newts. • Southeast Alaska is the northern range limit of the rough-skinned newt, Taricha granulosa. • The newts contain highly variable concentrations of tetrodotoxin. • Genetic testing using DNA-microsatellites provided no correlation of toxin-levels and population structure. • Other, still unknown, endo- and/or exogenous factors may trigger the high variability of the toxin-levels in the newts. [ABSTRACT FROM AUTHOR]

Salamandrina is the only extant representative of the Salamandrininae, characterised by a unique suite of morphological, ecological, and ethological features. The Salamandrina species are endemic of Italy, although their past distribution included different areas of Europe. Fossil remains belonging to this genus were previously described from the Miocene of Hungary, Mio-Pliocene transition of Greece, and Pliocene of Sardinia. The fossil record of this genus is herein expanded, with detailed descriptions of pre-caudal and caudal vertebrae from the Miocene of Germany (Petersbuch), Greece (Ano Metochi), Sardinia (Oschiri), and Spain (La Gloria, La Roma, Masada Ruea, Puente Minero), and the Pleistocene of Italy (Montagnola Senese). Otic-occipitum complexes and limb elements referred to Salamandrina are also described from the Miocene of La Roma (Spain). All these remains are referred to Salamandrina sp., given that identification at the species level of the retrieved skeletal elements is not possible. Nevertheless, the fossil record clearly indicates that the current range of this genus is the result of a prolonged extirpation process that ultimately led to the significant shrinkage of the original range or, at least apparently, to a late dispersal into the Italian Peninsula. [ABSTRACT FROM AUTHOR]

Documenting the sex of individuals encountered during wildlife research and monitoring activities is important for understanding and tracking changes in populations. However, sexing salamanders can be particularly difficult because secondary sex characters are often subtle or only visible during the breeding season, and guidance on species-specific sex determination is lacking from most field guides. The purpose of this guide is to provide a reference to assist biologists in the Central Appalachian region with identifying sex of live adult salamanders. In the main text we provide summary tables and figures to serve as concise references in the field. We provide individual species accounts that contain concise yet comprehensive information for each species based on the published literature, as well as many images depicting sexually dimorphic characters. Our focal region encompasses partial or entire distributions for 56 species of salamanders in 5 families (Ambystomidae, Cryptobranchidae, Plethodontidae, Proteidae, and Salamandridae). We identified seven morphological characters that are strongly sexually dimorphic and useful for sexing live, nonanesthetized, adult salamanders in the field, with males of individual species exhibiting one to five of the characters. We identified more than 20 additional characters that are weakly sexually dimorphic, difficult to distinguish in the field, or species-specific. Our guide serves as a synthesis of sexually dimorphic characters for salamanders in Central Appalachia, and we anticipate it will have broad value for researchers, monitoring programs, and salamander enthusiasts in eastern and central North America. [ABSTRACT FROM AUTHOR]

During three consecutive years, we compared the reproductive characteristics and oviposition dynamics of two crested newt species, Triturus ivanbureschi and T. macedonicus. These two well-defined species are of special interest because of complex interactions at their contact zone, which include hybridization, species replacement and asymmetric mitochondrial DNA introgression. In common garden experiments, females were introduced to conspecific males, to males of other species and to hybrid males. We monitored the total number and size of the deposited eggs, as well as the dynamics and duration of oviposition during three consecutive years. The number of deposited eggs of Triturus species was much higher than previously reported. Triturus macedonicus lay more eggs that were of larger size in comparison to T. ivanbureschi. Also, the onset of T. macedonicus oviposition was considerably delayed compared to T. ivanbureschi. These are fundamental data, important for understanding complex species interactions in their contact zones.

Chironomid (Diptera: Chironomidae) larvae play a key role in aquatic food webs as prey for predators like amphibian and dragonfly larvae. This trophic link may be disrupted by anthropogenic stressors such as Bacillus thuringiensis var. israelensis (Bti), a biocide widely used in mosquito control. In a companion study, we recorded a 41% reduction of non-target larval chironomids abundance in outdoor floodplain pond mesocosms (FPMs) treated with Bti. Therefore, we examined the diet of two top predators in the FPMs, larvae of the palmate newt (Salamandridae: Lissotriton helveticus) and dragonfly (Aeshnidae: predominantly Anax imperator), using bulk stable isotope analyses of carbon and nitrogen. Additionally, we determined neutral lipid fatty acids in newt larvae to assess diet-related effects on their physiological condition. We did not find any effects of Bti on the diet proportions of newt larvae and no significant effects on the fatty acid content. We observed a trend in Aeshnidae larvae from Bti-FPMs consuming a higher proportion of large prey (Aeshnidae, newt, damselfly larvae; ~42%), and similar parts of smaller prey (chironomid, mayfly, Libellulidae, and zooplankton), compared to controls. Our findings may suggest bottom-up effects of Bti on aquatic predators but should be further evaluated, for instance, by using compound-specific stable isotope analyses of fatty acids or metabarcoding approaches., (© 2024. The Author(s).)

We describe a new species of the newt genus Tylototriton from Umphang Wildlife Sanctuary, Tak Province, western Thailand based on molecular and morphological evidence and named here as Tylototriton umphangensis sp. nov. The new species is assigned to the subgenus Tylototriton and differs from other species in having dark-brown to blackish-brown body and limbs, truncate snout, prominent antero-medial ends of the expansion of the dentary bones, laterally protruding quadrate regions, indistinct and small rib nodules, a well-segmented vertebral ridge, and rough dorsolateral bony ridges, which are steeper anterior, and curved medially at the posterior ends. The molecular data show that Tylototriton umphangensis sp. nov. differs from T. uyenoi sensu stricto by a 5% genetic sequence divergence of the mitochondrial NADH dehydrogenase subunit 2 region gene. The new species and T. uyenoi are both endemic to Thailand, distributed along the Northwest Thai (Dawna) Uplands of Indochina. To clarify the species boundary between Tylototriton umphangensis sp. nov. and T. uyenoi, additional field research is needed in adjacent areas. Tylototriton umphangensis sp. nov. is restricted to evergreen hill forests in Umphang Wildlife Sanctuary. We suggest that the new species should be classified as Endangered (EN) in the IUCN Red List. [ABSTRACT FROM AUTHOR]