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Artisanal cayote jam consumption is widespread in Latin-American countries. This jam is prepared from the pulp of Cucurbita ficifolia Bouché. Here, an artisanal cayote jam recipe and a reduced-calorie artisanal cayote jam recipe were defined through sensory analysis. The defined jams showed higher overall acceptance and flavour quality compared to commercial ones. The low-calorie jam showed overall acceptance similar to the artisanal product, with no differences in the flavour quality. The cayote jams were characterised by determining physicochemical parameters and microbial flora. Artisanal jams presented physicochemical properties similar to commercial products. The variability of °Brix was greater (40–67); pH were 4.7–5.4, aw were 0.832–0.940. Reduced-calorie jam had pH ≥ 5, °Brix of 30–46 and aw>0.920. The colour of the jams showed significant variability depending on the recipe: L* (26.1–48.1), a*(1.6–4.6), b*(2.1–14.9). Cayote jams were found to be rich in carbohydrates (31–70%) and energy value (134–290 kcal/100 g), with lower contents of fibre, sodium, fat, and protein. The microbiological analysis indicate that there are no risks related to the consumption of cayote jams, although there are indications of potential quality deterioration during subsequent storage due to the development of yeasts and when no preservatives are used in the jam recipes. The characteristic microflora of cayote jams was composed by Penicillium sp., Aspergillus sp., Cephalosporium acremonium, Candida sp. and Rhodotorula sp. These results will benefit artisanal cayote jam producers in Latin-American countries and other regions where C. ficifolia is cultivated, by highlighting the autochthonous value of cayote and contributing to a nutritionally diverse diet. [ABSTRACT FROM AUTHOR]

While the use of food additives is common manufacturing practice, the levels used in food have to be compliant with the prescribed legislation. For fast control of present levels of food additives in products, ultra-high performance liquid chromatography coupled to tandem mass spectrometry with a triple quadrupole linear ion trap (QTRAP) mass analyser was applied to develop a method for the simultaneous determination of 41 frequently added food additives and flavourings, including 16 water-soluble colourants, 14 illegal dyes, 7 sweeteners, 2 preservatives, and 2 purine alkaloids. The method was validated using energy drink, chilli powder, condiment, and jelly sweets as food sample matrices. The average recovery values were in the range of 70‒120%, and the relative standard deviations were less than 10% for the majority of the analytes. The validated method was applied for the analysis of 134 samples from the Czech market. [ABSTRACT FROM AUTHOR]

The purpose of this article is to present selected food additives as disruptors of normal intestinal homeostasis with a potential impact on the development of metabolic dysfunction-associated steatotic liver disease (MASLD). A comprehensive literature search was conducted in three major electronic databases: PubMed, ScienceDirect, and Google Scholar. MASLD is a prevalent liver condition that is closely related to the global rise in obesity. Its pathogenesis is multifactorial, with genetic, environmental, and metabolic factors playing a key role. The "multiple-hit" hypothesis suggests that a Western-style diet, rich in ultra-processed foods, saturated fats, and food additives, combined with low physical activity, contributes to obesity, which promotes lipid accumulation in the liver. Recent studies underscore the role of impaired intestinal homeostasis in the development of MASLD. Food additives, including preservatives, emulsifiers, and sweeteners, affect gut health and liver function. Selected preservatives inhibit pathogenic microorganisms but disrupt the intestinal microbiota, leading to changes in intestinal permeability and liver dysfunction. Some emulsifiers and thickeners can cause inflammation and alter the gut microbiome, contributing to liver steatosis. Furthermore, the use of sweeteners such as sucralose and aspartame has been linked to changes in liver metabolism and intestinal microbial composition, which in turn promotes metabolic disorders. [ABSTRACT FROM AUTHOR]

This study examined the effects of alternative sweeteners—allulose, stevia, and xylose—on the physicochemical, textural, and sensory properties of Yanggaeng fortified with 2% Corni fructus powder (CF). A proximate composition analysis revealed that Yanggaeng treated with alternative sweeteners had a significantly higher moisture content and a lower carbohydrate content than that treated with sugar. Colorimetric analysis showed that the xylose treated Yanggaeng decreased lightness (L*) and increased redness (a*) and yellowness (b*), likely due to enhanced browning reactions. The xylose treated Yanggaeng exhibited the highest Brix values and antioxidant activities, including DPPH and ABTS radical scavenging and ferric reducing antioxidant power (FRAP). Although the allulose treated group had the highest total phenolic content, its radical scavenging activity was lower than the xylose treated Yanggaeng. Xylose produced a firmer and more cohesive gel matrix, increasing hardness, gumminess, and chewiness, whereas stevia resulted in a softer, less chewy texture. In sensory evaluations, the xylose treated Yanggaeng scored higher for color, flavor, sweetness, overall acceptance, and purchase intent than other groups. These findings suggest that xylose is the most suitable alternative sweetener for CF-enhanced Yanggaeng, offering reduced caloric content, enhanced antioxidant properties, improved texture, and superior consumer acceptance. [ABSTRACT FROM AUTHOR]

Biscuits are characterized by their popular sweet taste, but they have a poor nutritional profile due to their high sugar and saturated fat content, along with low fibre levels. Their sweetness primarily comes from sucrose, which not only determines the flavour but also performs several technological functions, making it difficult to replace in pastry products. Commercial sweeteners and soluble fibres designed for pastry products are available. Therefore, it is necessary to test the feasibility of using these ingredients in biscuit formulations and assess their impact on biscuit quality. Concurrently, the correlation analysis of dough rheological parameters, structure, and instrumental texture parameters with sensory characteristics will help identify which parameters are strongly correlated and can be used to predict biscuit quality. The purpose of this study was to investigate the dough rheological properties, structure, texture, and sensory characteristics of biscuits in which sucrose was replaced by the commercial sweeteners Tagatesse, maltitol, and erythritol–stevia, with the addition of soluble fibres Nutriose® FB (wheat fibre) and PromOat 35 (oat fibre). At the same time, a correlation analysis was conducted between dough rheological parameters (stickiness, work of adhesion, dough strength) and biscuit quality parameters, such as water activity, water content, colour, texture (pore area, pore shape, pore elongation), and instrumental texture properties (hardness, brittleness, number of acoustic emission (AE) events, AE event energy), with sensory discrimination evaluated through a consumer test. The use of wheat and oat fibres in combination with sucrose resulted in biscuits with lower apparent density, increased porosity, and weaker texture (fracturability, hardness, number of AE events), yet they had better sensory properties compared to biscuits containing sucrose alone. Replacing sucrose with sweeteners combined with fibres led to a deterioration in the sensory quality of the biscuits and a significant change in the dough's rheological properties. Regardless of the type of sweetener, biscuits with wheat fibre were rated better than those with oat fibre. Of the tested sweeteners, only maltitol combined with wheat fibre resulted in a sensory quality similar to that of sucrose biscuits. Correlation analysis of all measured biscuit quality parameters showed that only the number of AE events had a strong positive correlation with all tested sensory attributes. Porosity was only correlated with sensory crispness, and fracturability was correlated with sweetness, taste, and overall acceptability. Therefore, it appears that the number of AE events recorded at the time of breaking may be a reliable parameter for predicting biscuit quality. [ABSTRACT FROM AUTHOR]

Apple and beetroot pomace flour (APF and BPF), along with two sweeteners, sucrose and a blend of sucrose substitutes (erythritol, stevia, inulin, and fructose), were simultaneously incorporated into three matrices formulated with agar, pectin, or gelatin as gelling agents. The aim was to produce jelly candies with high content of dietary fiber and dietary phenolics, and reduced energy value. The simultaneous incorporation of sucrose substitutes and pomace flour resulted in decrease of Carb:Fiber and Sugar:Fiber Ratio to extremely low values of 2.7–3.4 and 1.3–1.6 respectively, as well as in Energy:Fiber Ratio decrease to 9.2–12.3 kcal/g DF. Relative Antioxidant Capacity Index (RACI), as indicator of antioxidant potential, was calculated by assigning equal weight to Folin–Ciocâlteu, DPPH and FRAP assays applied upon in vitro digestion of 18 formulations of jelly candies. Results obtained for formulations with and without sucrose, as well as with and without APF or BPF, enabled insight into effects of pomace flour addition and sucrose substitution in each gelling matrix on functional properties. The incorporation and the substitution impact on postprandial glucose response were followed in vivo. Their superimposing resulted in glycemic index below 30 and low glycemic load. Efficiency of applied approach in functionalization of confectionery burden with energy and minimization of glucose spike represent an example of agro-residues re-introduction with the highest potential contribution to anti-obesity strategy. [ABSTRACT FROM AUTHOR]

Purpose: There are increasing concerns regarding detrimental health effects of added sugar in food and drink products. Non-nutritive sweeteners (NNS) and sweetness enhancers (SE) are seen as viable alternatives. Much work has been done on health and safety of NNS&SE when consumed in place of sugar, but very little on their sustainability. This work aims to bridge that gap with an environmental study of replacing added sugar with NNS&SE in the context of drink and yoghurt. Methods: A life cycle assessment (LCA) approach was used to compare environmental impact of a drink and yoghurt, sweetened with sucrose, to those sweetened with NNSs or an SE: stevia rebaudioside A, sucralose, aspartame, neotame, and thaumatin. Primary ingredients data were taken from preparation of foodstuffs for clinical trials. Results are reported via the ReCiPe 2016 (H) method, with focus on land use, global warming potential (GWP), marine eutrophication, mineral resource scarcity, and water consumption. Impacts are reported in terms of 1 kg product. Scenarios explore sensitivity of the LCA results to change in background processes, functional unit, and sweetener type. This research was conducted as part of the EU Horizon 2020 project SWEET (sweeteners and sweetness enhancers: impact on health, obesity, safety, and sustainability). Results and discussion: Replacing sugar with an NNS or part-replacing with an SE is shown to reduce environmental impact across most impact categories, for example, on a mass basis, GWP for a drink reduces from 0.61 to approx. 0.51 kgCO2-eq/kg and for a yoghurt from 4.15 to approx. 3.73 kgCO2-eq/kg. Variability in environmental impact is shown to be relatively small between the NNSs, indicating that choice of NNS is less important than the reformulation changes required to accommodate the loss of sugar. Reporting impact in terms of calorie density, instead of mass, shows greater reduction in environmental impact when using an NNS or SE and shows how important functional unit is when reporting impact of these products. Conclusion: This study is the first to compare food or drink products sweetened with sugar, NNS, or SE. Results show that there is great potential to reduce environmental impact of sweetened drinks and yoghurts. Moreover, the choice of NNS does not greatly affect the environmental impact of either product. Therefore, this research shows that choices relating to replacing added sugar may be based more upon health or formulation needs and less on environmental concerns. [ABSTRACT FROM AUTHOR]

Background: d-Tryptophan is recognised for its unique physiological properties. In this study, we aimed to explore the dynamic trends and emerging topics in d-tryptophan research to offer fresh perspectives for future studies. Methods: Employing bibliometric analysis, we examined the literature on d-tryptophan indexed in the Web of Science Core Collection from January 1987 to December 2023. The "Bibliometrix" R package and CiteSpace were utilised for data processing. Results: Analyses of 865 publications revealed 2209 keywords, 4068 authors, 2094 institutions, and contributors from 302 regions. The USA was at the forefront of publications concerning d-tryptophan, but the European Journal of Pharmacology, Journal of Biological Chemistry, and Journal of Medicinal Chemistry were notable for their contributions, co-citations, and impact, respectively. This literature review reveals that since 1987, studies have developed from a focus on d-tryptophan metabolism to the exploration of its functions in organic and medicinal chemistry and food science. Recent findings highlight the potential of d-tryptophan as a non-nutritional sweetener and food preservative as well as its role in inhibiting the growth of bacterial biofilms. Additionally, its immunomodulatory properties are being investigated in relation to allergic diseases. Furthermore, d-tryptophan plays a role in the therapy of atherosclerosis, osteoporosis, tuberculosis, and cancer. Conclusion: The results of bibliometric analysis highlight that future research should focus on the biological functions of d-tryptophan as a food preservative and its use in immunomodulation and drug development, providing strong guidance for future research. [ABSTRACT FROM AUTHOR]

We examined the association between low‐calorie sweeteners (LCS) consumption during preconception, pregnancy, and breastfeeding and child health outcomes. A systematic search of electronic databases in PubMed, Embase, Cumulated Index to Nursing and Allied Health Literature, the Cochrane Library, Scopus, Web of Science, PsycINFO, ProQuest Health and Medical, ClinicalTrials.gov, and Google Scholar was conducted up to 21 September 2023. A random effects model with restricted maximum likelihood estimation was used for the meta‐analysis. Seventeen eligible studies were included. The standardised mean difference (SMD) and 95% confidence interval (CI) in birth weight between those who frequently consumed LCS (≥1 serve/day) during pregnancy and those who did not consume LCS was 0.04 (0.00, 0.08) (four cohort studies). Any LCS consumption during pregnancy compared with no consumption was not associated with birth weight [SMD (95% CI) = 0.03 (−0.03, 0.08)] (four cohort studies). Any LCS consumption during pregnancy was not associated with body mass index z‐scores. The weighted mean difference (95% CI) was 0.00 (−0.05, 0.06) at birth, 0.06 (−0.29, 0.40) at 6 months, −0.04 (−0.19, 0.10) at 1 year, 0.00 (−0.16, 0.17) at 3 years, and 0.10 (−0.15, 0.34) at 7 years of the child age, compared with no intake (five cohort studies). The odds of being overweight at 1 year among children exposed to LCS during pregnancy was 1.19 (OR [95% CI]: 1.19 [0.81, 1.58]) compared with unexposed children (two cohort studies). The effect sizes were not precise for all the outcomes as the 95% CI indicated the effect estimates could range from small protective to a higher risk. The effect of LCS consumption on child behaviour and cognition was inconsistent. There is not enough evidence to confirm LCS consumption during pregnancy affects birth weight and risk of overweight in children. However, frequent consumption increased birth weight and the risk of overweight at different ages, though the effects were imprecise. More robust research evidence is required as the quality of evidence is low. Key messages: Evidence regarding the effect of perinatal low‐calorie sweeteners (LCS) consumption and child health is limited and inconclusive.Our systematic review and meta‐analysis showed that any LCS consumption during pregnancy was not associated with birth weight and the risk of overweight in childhood. However, frequent LCS consumption during pregnancy was associated with a small but imprecise increase in birth weight and body mass index z‐score at different ages.Evidence regarding the effect of maternal LCS consumption on child behaviour, cognition, and the risk of other chronic disease is limited.The overall quality of the evidence remains low, warranting further high‐quality research. [ABSTRACT FROM AUTHOR]

Obesity, diabetes, and tooth decay are directly related to high sugar intake. Therefore, it is important to reduce sugar consumption by replacing it with foods that provide less energy. At this juncture, xylitol provides fewer calories than sucrose and helps preventmtooth decay. Additionally, probiotics have many positive effects on host health. This study aimed to produce a tooth-friendly probiotic pudding by incorporating xylitol (25, 50, 75 and 100%) and the probiotics L. rhamnosus and S. boulardii. The effects of adding xylitol and probiotics on pH levels, syneresis, rheological properties, probiotic viability, and consumer acceptance were evaluated. The addition of xylitol resulted in a slight increase in pH, whereas the inoculation of probiotic cultures led to a notable reduction in pH values (P ≤ 0.05). The addition of xylitol reduced the syneresis levels of the puddings. The addition of xylitol resulted in an increase in the consistency values, whereas the incorporation of probiotics resulted in a reduction. Although a decline in the viability was observed for both L. rhamnosus and S. boulardii during storage, the viability of probiotics remained above the desired level (8.91 and 6 log CFU/g for both probiotics, respectively). The sensory acceptability of probiotic-free puddings was satisfactory, whereas probiotic puddings were not deemed acceptable by the panelists. Finally, this study demonstrated that xylitol could be an alternative sweetener to sucrose in the production of tooth-friendly pudding and that the pudding could be used as an alternative probiotic carrier. [ABSTRACT FROM AUTHOR]

AIM. To study the effect of the non-calorie sweetener erythritol and the combination of erythritol and sucrose on postprandial secretion of insulin and peptide-YY (PYY).MATERIALS AND METHODS. A comparative study was conducted to assess the effect of erythritol, sucrose and a combination of erythritol and sucrose on postprandial glucose, IRI and PYY levels. The participants were selected from among healthy volunteers, aged 18 to 35 years. If the participants met the selection criteria, further stages of the study were carried out. During the study, each participant took a solution of sucrose 75 g, erythritol 75 g or a mixture of sucrose and erythritol (75 g and 25 g, respectively). Further determination of glucose and insulin was carried out every 30 minutes, so glucose, insulin and PYY values were determined initially and at the 30th, 60th, 90th and 120th minutes.RESULTS. Data were obtained confirming the absence of the effect of erythritol on postprandial secretion of insulin and glucose. In our work, we also demonstrated a decrease in postprandial glycemia when taking sucrose and erythritol together. Sucrose and erythritol equally stimulated PYY secretion.CONCLUSION. Our data show that erythritol can be considered as an optimal sugar substitute in people with impaired carbohydrate metabolism and obesity.

Several studies have explored the effects of single or binary mixtures of sweeteners on both healthy individuals and those with diabetes. However, there is limited research on the impact of a combination of four sweeteners supplemented with dietary minerals. Steviol glycosides, extracted from Stevia rebaudiana, offer a zero-calorie sweetness that exerts minimal influence on blood glucose levels. When combined with other sweeteners, they can reduce the required quantity of each component thus mitigating the potential side effects. Furthermore, the incorporation of chromium picolinate into sweeteners may enhance insulin sensitivity and glucose metabolism and diminish insulin resistance in both diabetic and non-diabetic individuals. This study aimed to evaluate the effects of commercial mixed sweeteners (acesulfame-K, sucralose, sorbitol, and steviol glycoside) supplemented with chromium picolinate (MSSC) on diabetes-related markers and complications in healthy and type 2 diabetic rats (T2D). Over six weeks, diabetic rats received daily oral administration of MSSC at a standard dosage. The results demonstrated that MSSC significantly reduced weight loss in diabetic rats, lowered fasting blood glucose levels, enhanced hexokinase activity, and improved pancreatic antioxidative capacities. Additionally, MSSC treatment led to notable reductions in serum triglycerides, cholesterol, malondialdehyde (MDA), and LDL cholesterol levels. The treatment also modulated specific renal function parameters, and moderately reversed the necrotic architectures of the liver and pancreatic β cells. These results indicate that long-term administration of MSSC may alleviate certain diabetic complications without adverse effects on non-diabetic individuals. Further clinical studies are strongly recommended to evaluate the safety and efficacy of MSSC in diverse populations.

We aimed to evaluate the association of coffee consumption with different additives, including milk and/or sweetener (sugar and/or artificial sweetener), and different coffee types, with new-onset acute kidney injury (AKI), and examine the modifying effects of genetic variation in caffeine metabolism. 194 324 participants without AKI at baseline in the UK Biobank were included. The study outcome was new-onset AKI. During a median follow-up of 11·6 years, 5864 participants developed new-onset AKI. Compared with coffee non-consumers, a significantly lower risk of new-onset AKI was found in coffee consumers adding neither milk nor sugar to coffee (hazard ratio (HR), 0·86; 95 % CI, 0·78, 0·94) and adding only milk to coffee (HR,0·83; 95 % CI, 0·78, 0·89), but not in coffee consumers adding only sweetener (HR,1·14; 95 % CI, 0·99, 1·31) and both milk and sweetener to coffee (HR,0·96; 95 % CI, 0·89, 1·03). Moreover, there was a U-shaped association of coffee consumption with new-onset AKI, with the lowest risk at 2–3 drinks/d, in unsweetened coffee (no additives or milk only to coffee), but no association was found in sweetened coffee (sweetener only or both milk and sweetener to coffee). Genetic variation in caffeine metabolism did not significantly modify the association. A similar U-shaped association was found for instant, ground and decaffeinated coffee consumption in unsweetened coffee consumers, but not in sweetened coffee consumers. In conclusion, moderate consumption (2–3 drinks/d) of unsweetened coffee with or without milk was associated with a lower risk of new-onset AKI, irrespective of coffee type and genetic variation in caffeine metabolism. [ABSTRACT FROM AUTHOR]

Background: Foods with protein claims (PCs) targeted at the general population are increasingly sought after by consumers because they think they are healthy. However, they may contain other nutrients that pose a health risk. Objectives: Therefore, the aim of this work was to carry out a comprehensive evaluation of foods with PC and compare them with those without these claims. Methods: The Spanish Food Database, BADALI, was used for this purpose. We studied 4325 processed foods of 12 different types. Thirteen percent had PCs and more than half of them were fortified with proteins (60.4%). Plant proteins were added more frequently than animal proteins. Protein values were higher in foods with PCs, particularly in those that were fortified. Differences in other nutrients were also observed depending on the food type. The healthiness of foods was assessed using the Pan American Health Organization Nutrient Profile Model and 90.8% of those bearing PCs were classified as "less healthy". More than 50% were high in fat or high in sodium; around one in four were high in free sugar or saturated fat and one in five had sweeteners. Foods with PCs had 13.1% more "less healthy" items than those without PCs. The proportion of items high in fat or high in sodium were also larger and more of them had sweeteners. In contrast, the proportion of foods high in free sugar and high in saturated fat was lower among those with PCs. Conclusions: Therefore, the perception that foods with PCs are healthy is incorrect, and consuming them may pose an additional health risk. [ABSTRACT FROM AUTHOR]

Currently, lipid/polymer membranes are used in taste sensors to quantify food taste. This research aims to improve sweetness sensors by more selectively detecting uncharged sweetening substances, which have difficulty obtaining a potentiometric response. Lipid/polymer membranes with varying amounts of tetradodecylammonium bromide (TDAB) and 1,2,4-benzene tricarboxylic acid (trimellitic acid) were prepared. The carboxyl groups of trimellitic acid bind metal cations, and the sweetness intensity is estimated by measuring the potential change, as a sensor response, when these cations are complexed with sugars. This research showed that the potential of a sensor using the membrane with enough trimellitic acid in a sucrose solution remained constant, regardless of TDAB amounts, but the potential in the tasteless, so-called reference solution, depended on TDAB. By optimizing the content of TDAB and trimellitic acid, a sensor response of −100 mV was achieved, which is over 20% more sensitive than a previous sensor. This sensor also demonstrated increased selectivity to sweetness, with similar interference from other tastes (saltiness, sourness, umami, and bitterness) compared to previous sensors. As a result, the sensitivity to sweetness was successfully improved. This result contributes to the development of novel sensors, further reducing the burden on humans in quality control and product development. [ABSTRACT FROM AUTHOR]

Introduction: The aim of the study was to evaluate the modulating effects of five commonly used sweetener (glucose, inulin, isomaltulose, tagatose, trehalose) containing mouth rinses on the oral microbiome. Methods: A single-centre, double-blind, parallel randomized clinical trial was performed with healthy, 18–55-year-old volunteers (N = 65), who rinsed thrice-daily for two weeks with a 10% solution of one of the allocated sweeteners. Microbiota composition of supragingival dental plaque and the tongue dorsum coating was analysed by 16S RNA gene amplicon sequencing of the V4 hypervariable region (Illumina MiSeq). As secondary outcomes, dental plaque red fluorescence and salivary pH were measured. Results: Dental plaque microbiota changed significantly for two groups: inulin (F = 2.0239, p = 0.0006 PERMANOVA, Aitchison distance) and isomaltulose (F = 0.67, p = 0.0305). For the tongue microbiota, significant changes were observed for isomaltulose (F = 0.8382, p = 0.0452) and trehalose (F = 1.0119, p = 0.0098). In plaque, 13 species changed significantly for the inulin group, while for tongue coating, three species changed for the trehalose group (ALDEx2, p < 0.1). No significant changes were observed for the secondary outcomes. Conclusion: The effects on the oral microbiota were sweetener dependant with the most pronounced effect on plaque microbiota. Inulin exhibited the strongest microbial modulating potential of the sweeteners tested. Further full-scale clinical studies are required. [ABSTRACT FROM AUTHOR]

SUMMARY: Licorice is one of the most extensively studied medicinal plants in the world, whose roots and rhizomes have long been used as both a sweetener and an essential component in numerous herbal preparations. However, the genus Glycyrrhiza has a complex composition, and the interspecies chromosomal relationships, origin, and evolution are still largely unclear. Here, we develop a set of whole‐chromosome painting probes that allowed identification of all eight chromosomes of licorice on same metaphase chromosomes. Comparative chromosome painting analyses in seven different Glycyrrhiza species revealed that the genus Glycyrrhiza maintained extraordinarily conserved chromosomal synteny after about 3–12 million years of divergence. No cytologically visible inter‐chromosomal rearrangements were identified in any species. By comparative chromosomal karyotype analyses, we revealed interspecific chromosome evolutionary relationships and dramatic variable chromosomal karyotype after independent divergence and demonstrated that G. prostrate was the most closely related to the ancestral type among the seven Glycyrrhiza species. Furthermore, we also discovered a G. glandulosa seed with distinct triploid‐genome for the first time in China, suggesting the existence of a polyploid evolutionary pathway in the genus Glycyrrhiza, which challenges the previous notion that only diploids of licorice existed in nature. This study expands our knowledge of the chromosome evolution of licorice and will lay an important foundation for the genome origin and evolution studies in the genus Glycyrrhiza. Significance Statement: Whole‐chromosome oligo‐painting in licorice reveals interspecific chromosome evolutionary features, phylogenetic relationships, karyotype variation, and polyploid evolution. [ABSTRACT FROM AUTHOR]

Accurate identification of the taste of compounds has helped in the screening and development of new sweeteners. This study proposes a deep learning model for sweetener identification based on transfer learning and attention mechanism. The Squeeze-and-Excitation (SE) attention mechanism is incorporated into the pre-trained Residual Network-50 (ResNet-50) model, resulting in SE-ResNet-50. Additionally, the Convolutional Block Attention Module (CBAM) is integrated to generate the CBAM-SEResNet-50 model for sweetener identification. This study divided the taste molecule dataset into two parts: Cross-Validation (CV) dataset and Hold-out test dataset. The effectiveness of the algorithm was verified using both the 5-fold CV and the Hold-out test methods. The experimental results demonstrate that the CBAM-SEResNet-50 model achieves an accuracy of 0.956 and an Area Under the Receiver Operating Characteristic Curve (AUROC) of 0.972 on the Hold-out test dataset. In the case of the 5-fold CV, the accuracy is 0.944 and the AUROC is 0.969. [ABSTRACT FROM AUTHOR]

Aspartame is a high potency artificial sweetener which is popularly used in foods and beverages. The species-dependent sweet taste toward aspartame has not been completely understood. In a recent publication, we reported that the prosimians Lemuriformes species, which are proposed as aspartame nontasters, could taste aspartame based on the sequence and structure analysis. In this study, by mutagenesis, cell-based functional analysis and molecular simulations, we reveal that Lemuriformes species can respond to aspartame at the cell-based receptor activity level. Furthermore, it is proved that the conserved critical residues D142 and S40 mediate the species-dependent sweet taste toward aspartame. This research provides a deeper insight on the species taste, structure-activity relationship and evolution for eliciting the sweetness of this important synthetic sweetener. • The Tas1R2/Tas1R3 of Lemuriformes species Coquerel's sifaka can respond to aspartame. • Lemuriformes species gray mouse and ring-tailed lemurs are also aspartame tasters. • Two critical residues D142 and S40 mediate the species-dependent taste to aspartame. • Sequence alignment indicates conserved residues involved in aspartame recognition. • Molecular simulations show essential residues of receptor interacting with aspartame. [ABSTRACT FROM AUTHOR]

Several studies have explored the effects of single or binary mixtures of sweeteners on both healthy individuals and those with diabetes. However, there is limited research on the impact of a combination of four sweeteners supplemented with dietary minerals. Steviol glycosides, extracted from Stevia rebaudiana, offer a zero-calorie sweetness that exerts minimal influence on blood glucose levels. When combined with other sweeteners, they can reduce the required quantity of each component thus mitigating the potential side effects. Furthermore, the incorporation of chromium picolinate into sweeteners may enhance insulin sensitivity and glucose metabolism and diminish insulin resistance in both diabetic and non-diabetic individuals. This study aimed to evaluate the effects of commercial mixed sweeteners (acesulfame-K, sucralose, sorbitol, and steviol glycoside) supplemented with chromium picolinate (MSSC) on diabetes-related markers and complications in healthy and type 2 diabetic rats (T2D). Over six weeks, diabetic rats received daily oral administration of MSSC at a standard dosage. The results demonstrated that MSSC significantly reduced weight loss in diabetic rats, lowered fasting blood glucose levels, enhanced hexokinase activity, and improved pancreatic antioxidative capacities. Additionally, MSSC treatment led to notable reductions in serum triglycerides, cholesterol, malondialdehyde (MDA), and LDL cholesterol levels. The treatment also modulated specific renal function parameters, and moderately reversed the necrotic architectures of the liver and pancreatic β cells. These results indicate that long-term administration of MSSC may alleviate certain diabetic complications without adverse effects on non-diabetic individuals. Further clinical studies are strongly recommended to evaluate the safety and efficacy of MSSC in diverse populations. [ABSTRACT FROM AUTHOR]

There is significant evidence that an unhealthy diet greatly increases the risk of complications during pregnancy and predisposes offspring to metabolic dysfunction and obesity. While fat intake is typically associated with the onset of obesity and its comorbidities, there is increasing evidence linking sugar, particularly high fructose corn syrup, to the global rise in obesity rates. Furthermore, the detrimental effects of added sugar intake during pregnancy on mother and child have been clearly outlined. Guidelines advising pregnant women to avoid food and beverages with high fat and sugar have led to an increase in consumption of 'diet' or 'light' options. Examination of some human birth cohort studies shows that heavy consumption (at least one beverage a day) of non-nutritive sweetener (NNS) containing beverages has been associated with increased risk of preterm birth and increased weight/BMI in male offspring independent of maternal weight, which appears to be offset by breastfeeding for 6 months. Rodent models have shown that NNS exposure during pregnancy can impact maternal metabolic health, adipose tissue function, gut microbiome profiles and taste preference. However, the mechanisms underlying these effects are multifaceted and further research, particularly in a translational setting is required to fully understand the effects of NNS on maternal and infant health during pregnancy. Therefore, this review examines maternal sweetener intakes and their influence on fertility, maternal health outcomes and offspring outcomes in human cohort studies and rodent models. [ABSTRACT FROM AUTHOR]

Featured Application: This research demonstrates the potential of Terminalia catappa kernel flour as a novel, sustainable ingredient in gluten-free cookie formulation, offering enhanced nutritional value through significant antioxidant properties and dietary fiber content. The findings support the valorization of this underutilized tropical resource in functional food development, particularly in regions where T. catappa is abundant but currently underexploited.Terminalia catappa (tropical almond) represents an underutilized resource with potential applications in functional food development. This study investigated the technological properties and bioactive characteristics of T. catappa kernel flour and its application in cookie formulation. The research examined the techno-functional properties, pasting behavior, and bioactive profile of T. catappa flour and its blends with different sweeteners (erythritol and cane sugar at 5% and 15% concentrations). Cookies were formulated using optimized ingredients, and their quality parameters were evaluated through physical, chemical, and sensory analyses. T. catappa flour demonstrated significant water holding capacity (4.48 g H2O/g DM) and notable antioxidant activity in both aqueous and ethanolic extracts (DPPH: 1.95–3.35 mg TE/g DM). The addition of sweeteners influenced pasting properties, with higher concentrations generally reducing peak viscosity and pasting temperature. Developed cookies exhibited stable water activity (0.294–0.320) over one month of storage and contained substantial dietary fiber (5.018 g/100 g). Sensory evaluation revealed superior acceptability for thicker (10 mm) cookies, particularly in texture and appearance attributes. This study establishes T. catappa kernel flour as a promising functional ingredient for gluten-free bakery applications, offering both technological functionality and bioactive properties suitable for health-conscious product development. [ABSTRACT FROM AUTHOR]

So far, the use of artificial low‐calorie sweeteners, like sucralose, saccharin, and so on, to replace the conventional‐based sugars has not succeeded due to the long‐term adverse health effects, for example, hypertension, and not well‐known safety stand. In this review, we discussed the next generation SvGl (rebaudioside M [Reb M]), their biosynthetic pathway in plant, high‐yield production via microbial fermentation and enzyme engineering, physicochemical properties, taste modification, kinetic metabolism, application in food and beverages, safety and toxicological evaluation, regulation and dosage recommendation, and health benefits. In stevia, the biosynthesis of stevia glycosides, especially Reb M, is derived from the bifurcation of the pathway leading to gibberellin, followed by subsequent enzymatic modification of rubusoside. Reb M is more economically produced via microbial fermentation of modified yeast Yarrowia lipolytica and enzymatic bioconversion of rebaudioside A (Reb A) or Reb E. Reb M can serve as a suitable alternative to the conventional‐based sugars. [ABSTRACT FROM AUTHOR]

The production of instant weaning food porridge is one of the most popular research topics. Granulation is the preferred method for producing instant foods. The advantages of granulation include easy storage, dissolution and shelf life. Whole-grain flour and high -fiber materials, in addition to replacing sugar with natural sweeteners, are a viable solution to reduce the adverse effects of a high -calorie diet. In this study, the effect of the granule content of almond oil cake oatmeal (65 - 70%), grape juice powder (25 -30%) and sugar (0 -5%) on the physicochemical properties, color brightness, emulsifying activity, stability and sensory parameters of the final porridge was investigated based on the statistical mixing design. According to the optimization results, the recipe of the instant porridge contains 69.9% almond oatmeal granules, 9.26% grape juice powder and 3.89% sugar to achieve emulsifying ability, emulsion stability, color brightness and overall acceptability of 65.82, 64.16, 3.75 and 89.89, respectively. According to the optimization results, for emulsifying ability, emulsion stability, color brightness and total acceptance 65.82, 64.16, 3.75 and 89.89, respectively, the instant porridge formulation was found to contain 69.9% almond oatmeal granules, 9.26% grape juice powder and 3.13% sugar. The fluctuations in blood sugar after 120 minutes of consumption of the optimal sample were significantly lower than in consumers with glucose sugar. Generally speaking, the combination of extrusion, foam mat and granulation technologies for the production of instant weaning food is considered an innovative idea for the development of healthy food technology. [ABSTRACT FROM AUTHOR]

Low- and no-calorie sweeteners (LNCSs) impart sweetness while providing little or no energy. Their safety and weight management efficacy remain unsettled science that leaves open questions among consumers, researchers, clinicians, and policy makers. The objective of this narrative review is to provide a critical consideration of the safety and efficacy of weight management evidence for LNCSs that have been reviewed/approved by the US Food and Drug Administration and have the highest frequency of use: acesulfame potassium, allulose, aspartame, erythritol, monk fruit, saccharin, stevia, sucralose, and xylitol. Safety assessments by the authoritative bodies for the World Health Organization, European Union, and United States were reviewed. Additionally, emerging topics of interest regarding the safety of these sweeteners commonly cited in the recent literature or highlighted in the media are discussed. Collectively, authoritative assessments and the primary literature support the safety of the sweeteners reviewed herein, with high concordance of safety substantiation across authoritative bodies. Weight management efficacy, measured by various adiposity indices in epidemiological studies, ranges from no effect to a slight positive association. Clinical trials with various mixtures of LNCSs more consistently indicate LNCS use is associated with lower adiposity indices. The latter are ascribed greater evidentiary weight, and recent application of statistical methods to better correct for potential biases in cohort studies reveals they are more consistent with the clinical trial findings. Studies that investigated individual sweeteners were limited but suggestive of differing effects or lack of sufficient data to support any formal conclusions on their efficacy for weight management. Taken together, and consistent with the current 2020-2025 Dietary Guidelines for Americans, the evidence indicates LNCS use is safe and may aid weight management. [ABSTRACT FROM AUTHOR]

The quest for healthier beverage options has led to the development of low-calorie alternatives to conventional sugary drinks. This study focused on the use of ultrasonication-enzymatic extraction to produce a low-calorie ready-to-drink Kangra orthodox black tea with the natural sweetener Stevia rebaudiana. By employing sonication time (9 to 45 min) and temperature (35 to 55 °C) as independent variables, statistical analysis revealed significant effects of these parameters on key quality indicators. In the ultrasonicated-assisted enzymatic extraction, the optimal conditions were 27.58 min at 45.06 °C with 0.0095% Tannase, to maximize the water extract content, total phenolic content, antioxidant capacity, caffeine, tannins content, colour, theaflavins, thearubigins, total colour, brightness, a*, b* colour value and overall acceptability and to minimize the pH, tea cream, and colour value L* of the ultrasonicated-assisted enzymatic extraction of Kangra orthodox black tea. The predicted water extract was 47.69%, the total phenolic content was 233.77 µg GAE/100 mL, the antioxidant capacity was 78.50 mM TE/mL, caffeine was 35.30 µg CAE/100 mL, tannins were 167.14 µg TAE/100 mL, theaflavins were 0.88%, thearubigins were 8.49%, the total colour percentage was 9.22%, the brightness was 14.98%, a* was 5.02, b* was 8.88, the overall acceptability was 8.14, the pH was 5.61, tea cream was 0.36 g/100 mL, and the L* colour value was 23.66 under optimum ultrasonication-assisted enzymatic extraction conditions. To improve the overall palatability of the Kangra Orthodox ready-to-drink tea, sugar (7–11° Brix) was utilized to balance the taste. The optimal sugar concentration for ready-to-drink Kangra orthodox tea based on a sensory score of 10° Brix was the highest. The substitution of sucrose with stevia up to 60% led to an equi-sweet taste eliminating any characteristic bitter aftertaste, in addition to a substantial decline in energy value from 31.44 to 15.72 kcal/100 g. A low-calorie Kangra Orthodox ready-to-drink tea beverage was developed that contained a high level of functional compounds at a reduced energy level. This tea offers a healthy alternative to traditional sweetened beverages and is potentially appealing to a broad market of health-conscious consumers. [ABSTRACT FROM AUTHOR]

Artificial sweeteners are generally used and recommended to alternate added sugar for health promotion. However, the health effects of artificial sweeteners remain unclear. In this study, we included 6371 participants from the National Health and Nutrition Examination Survey with artificial sweetener intake records. Logistic regression and Cox regression were applied to explore the associations between artificial sweeteners and risks of cardiometabolic disorders and mortality. Mendelian randomisation was performed to verify the causal associations. We observed that participants with higher consumption of artificial sweeteners were more likely to be female and older and have above medium socio-economic status. After multivariable adjustment, frequent consumers presented the OR (95 % CI) for hypertension (1·52 (1·29, 1·80)), hypercholesterolaemia (1·28 (1·10, 1·50)), diabetes (3·74 (3·06, 4·57)), obesity (1·52 (1·29, 1·80)), congestive heart failure (1·89 (1·35, 2·62)) and heart attack (1·51 (1·10, 2·04)). Mendelian randomisation confirmed the increased risks of hypertension and type 2 diabetes. Moreover, an increased risk of diabetic mortality was identified in participants who had artificial sweeteners ≥ 1 daily (HR = 2·62 (1·46, 4·69), P = 0·001). Higher consumption of artificial sweeteners is associated with increased risks of cardiometabolic disorders and diabetic mortality. These results suggest that using artificial sweeteners as sugar substitutes may not be beneficial. [ABSTRACT FROM AUTHOR]

Sucrose provides both sweetness and energy by binding to both Venus flytrap domains (VFD) of the heterodimeric sweet taste receptor (T1R2/T1R3). In contrast, non-caloric sweeteners such as sucralose and aspartame only bind to one specific domain (VFD2) of T1R2, resulting in high-intensity sweetness. In this study, we investigate the binding mechanism of various steviol glycosides, artificial sweeteners, and a negative allosteric modulator (lactisole) at four distinct binding sites: VFD2, VFD3, transmembrane domain 2 (TMD2), and TMD3 through binding experiments and computational docking studies. Our docking results reveal multiple binding sites for the tested ligands, including the radiolabeled ligands. Our experimental evidence demonstrates that the C20 carboxy terminus of the Gα protein can bind to the intracellular region of either TMD2 or TMD3, altering GPCR affinity to the high-affinity state for steviol glycosides. These findings provide a mechanistic understanding of the structure and function of this heterodimeric sweet taste receptor. Sucrose and other non-caloric sweeteners can bind to different domains of the heterodimeric sweet taste receptor (T1R2/T1R3), resulting in different levels of sweetness. Here, the authors investigate the binding mechanism of various steviol glycosides, artificial sweeteners, and a negative allosteric modulator (lactisole) at four distinct binding sites of T1R2/T1R3 through binding experiments and computational docking studies, revealing multiple binding sites for the tested ligands and structural– function correlations of ligand–receptor interactions. [ABSTRACT FROM AUTHOR]

Simple Summary: This systematic review examines the effects of non-nutritive sweeteners (NNS) on pigs, focusing on growth performance, feed preference, gut health, and other clinical indicators. Sweeteners such as stevia, sucralose, and neotame have been tested in various studies to evaluate their influence on swine production. Results show that NNS supplementation generally improves growth performance and feed intake in pigs, with some studies reporting reduced diarrhea rates and improved gut health. However, the effects of NNS on gut microbiota are inconsistent, with some sweeteners promoting beneficial bacteria growth while others show minimal changes in microbial diversity. Despite these outcomes, research on the long-term effects of NNS on gut health and the immune system remains limited. This review highlights the need for further studies to explore the mechanisms behind NNS effects, especially in diverse dietary and environmental conditions. Identifying optimal types and dosages of NNS, along with understanding their interactions with the gut microbiome, will be crucial in determining their role as a dietary supplement in swine production. Non-nutritive sweeteners (NNS) have been investigated for their potential to improve feed palatability and growth performance in pigs, although their use in swine production remains limited. This systematic review evaluates the effects of NNS on pigs, drawing from 18 studies published between 1990 and 2024. Following the PRISMA guidelines and using the PICOS framework, a total of 448 papers were initially identified, of which 18 met the inclusion criteria for review. The results are mixed: some studies suggest that NNS like stevioside, sucralose, and neotame may improve performance and reduce diarrhea, while others show limited or no effects. The impact of NNS on gut microbiota is similarly inconsistent, with some sweeteners promoting beneficial bacterial growth, while others show minimal changes in microbial diversity. This review emphasizes the need for more research to clarify the effects of NNS in pigs, particularly the mechanisms behind their influence on growth and gut health. Additionally, further studies are needed to determine optimal dosages and assess the long-term impacts of NNS on pig immune function and overall health. The findings highlight the current gaps in knowledge and suggest that more evidence is needed to understand the role of NNS in swine nutrition. [ABSTRACT FROM AUTHOR]

d-Xylitol is a naturally occurring sugar alcohol present in diverse plants that is used as an alternative sweetener based on a sweetness similar to sucrose and several health benefits compared to conventional sugar. However, current industrial methods for d-xylitol production are based on chemical hydrogenation of d-xylose, which is energy-intensive and environmentally harmful. However, efficient conversion of l-arabinose as an additional highly abundant pentose in lignocellulosic materials holds great potential to broaden the range of applicable feedstocks. Both pentoses d-xylose and l-arabinose are converted to d-xylitol as a common metabolic intermediate in the native fungal pentose catabolism. To engineer a strain capable of accumulating d-xylitol from arabinan-rich agricultural residues, pentose catabolism was stopped in the ascomycete filamentous fungus Aspergillus niger at the stage of d-xylitol by knocking out three genes encoding enzymes involved in d-xylitol degradation (ΔxdhA, ΔsdhA, ΔxkiA). Additionally, to facilitate its secretion into the medium, an aquaglyceroporin from Saccharomyces cerevisiae was tested. In S. cerevisiae, Fps1 is known to passively transport glycerol and is regulated to convey osmotic stress tolerance but also exhibits the ability to transport other polyols such as d-xylitol. Thus, a constitutively open version of this transporter was introduced into A. niger, controlled by multiple promoters with varying expression strengths. The strain expressing the transporter under control of the PtvdA promoter in the background of the pentose catabolism-deficient triple knock-out yielded the most favorable outcome, producing up to 45% d-xylitol from l-arabinose in culture supernatants, while displaying minimal side effects during osmotic stress. Due to its additional ability to extract d-xylose and l-arabinose from lignocellulosic material via the production of highly active pectinases and hemicellulases, A. niger emerges as an ideal candidate cell factory for d-xylitol production from lignocellulosic biomasses rich in both pentoses. In summary, we are showing for the first time an efficient biosynthesis of d-xylitol from l-arabinose utilizing a filamentous ascomycete fungus. This broadens the potential resources to include also arabinan-rich agricultural waste streams like sugar beet pulp and could thus help to make alternative sweetener production more environmentally friendly and cost-effective. [ABSTRACT FROM AUTHOR]

In this work, the electrochemical determination of sucralose and aspartame in drinks has been theoretically evaluated. Considering the accepting character of both sucralose chlorine atom and aspartame molecule, cathodic process is preferred. The theoretical description for VO(OH)-assisted electroanalytical process has proven the efficiency of the cathodic electroanalytical process for both of the sweeteners, despite of the augmented probability of the oscillatory behavior. [ABSTRACT FROM AUTHOR]

This research aimed to assess the influence of drying temperature (50, 60 and 70 °C), honey/oat flour ratio (60:40, 50:50 and 40:60) and cocoa contents (5, 6.25 and 7.5 g/100 g) on the physical (color, moisture content, bulk density, flowability (Hausner ratio, Carr index), dispersibility, solubility, and particle size), chemical (total dissolved solids, conductivity, pH, amount of sugar, color, total polyphenolic content, and antioxidant activity), and sensory properties (powder appearance, color, odor; and beverage appearance, color, odor, sweetness, bitterness, taste, texture) of a newly developed cocoa powder mixture in which honey was used as a sweetener and oat flour as a filler. Also, a further aim of this study was to optimize the composition of the mixture based on chemical, physical and sensory properties. Based on the optimization results, the highest total polyphenolic content and antioxidant activity were achieved at 70 °C with a honey/oat ratio of 50% and a cocoa content of 7.5 g. Drying temperature has a significant effect on powder odor and beverage odor, as well as on beverage bitterness, while the honey/oat flour ratio has a significant effect on color, with primarily values L* and a*. The cocoa contents mostly affect total polyphenolic content and antioxidant activity. [ABSTRACT FROM AUTHOR]

Stevia rebaudiana is an herbal supplement used as a substitute for sugar in food processing. Considering the potentials of stevia as a zero-calorie-natural sweetener, this study aimed to assess the antioxidant and anti-hyperglycemic properties of stevia (Stevia rebaudiana Bertoni) leaf powder (SLP) to find its suitability to formulate low-calorie foods. Extracts of SLP obtained with hexane, ethyl acetate, and methanol were evaluated for DPPH, ABTS+, FRAP and enzyme inhibitory assays. Adopting a standard recipe, five different cookie types were prepared by incorporating varying proportions of SLP and evaluated by a 30 semi-trained panel. The nutritional composition and shelf-life stability were also evaluated. SLP had moisture (8.0 %), ash (13.5 %), crude protein (12.4 %), crude fat (6.9 %), crude fiber (18.3 %), and carbohydrate (40.9 %). Sensory results showed that the cookie formulated with the lowest amount of SLP (T1 = 2.0g) had the highest overall acceptability with the highest preference for appearance and smell. The cookie type formulated with the highest amount of SLP (T4 = 8.0g) had the highest crude protein (13.50%), crude fiber (21.76%), and lowest carbohydrate contents (40.88%). The keeping quality of the cookies remained acceptable within the storage period. These results suggest that acceptable quality cashew nut fortified cookies could be prepared by replacing sucrose with stevia leaf powder [ABSTRACT FROM AUTHOR]

This text discusses the issue of excessive sodium in processed foods in the US and its negative impact on health, particularly for racial and ethnic minority groups. The US Food and Drug Administration (FDA) has taken steps to reduce sodium, including setting voluntary targets for the industry. Data from 2022 show that about 40% of the targets set in 2021 had already been reached or were close. The FDA has issued new voluntary sodium reduction targets, which, if achieved, would reduce sodium intake to about 2750 mg/day. The FDA's efforts are part of a broader initiative to reduce diet-related diseases and promote health equity. The text also includes research articles on various topics related to food science and technology, such as preserving the quality of winter jujubes and using d-allulose as a low-calorie alternative in sponge cake. These articles provide valuable insights for the food industry. [Extracted from the article]

The aim of this research was to produce a diet beverage based on aloe vera and investigate its physicochemical, microbiological, antioxidant, and sensory properties. To achieve this goal, aloe vera gel at concentrations of 5%, 5.7%, and 10% underwent heat treatment at 80°C for 30 minutes (Treatment A) and 90°C for 3 minutes (Treatment B). Aspartame was added at concentrations of 100, 150, and 200 ppm, and the beverages were stored for a period of 31 days. The study was conducted using a fractional factorial design with 34 samples under investigation. Optimization was performed based on a maximum amount of 10 gr of aloe vera, 200 ppm of aspartame, and a minimum amount of 5 gr of aloe vera and 100 ppm of aspartame. The use of the sweetener aspartame was highly effective in improving the taste of the beverage, as it provides a much sweeter sensation compared to saccharide sugars and is beneficial for diabetic patients. Based on the microbiological test results, the heat treatment (pasteurization) was successful. Furthermore, sensory evaluation showed improvements in taste, aroma, texture, and mouthfeel with the addition of aloe vera gel to the samples with no undesirable characteristics observed. After determining the models and variable combinations using the Design Expert software, an optimized sample was introduced, containing 200 ppm of aspartame, 10 gr of aloe vera, and the optimized pasteurization process, which was Treatment B for 30 minutes. The results of this study indicate that a vegetable -based diet beverage containing aloe vera can be successfully produced as a beneficial product with desirable sensory characteristics that are acceptable to consumers. [ABSTRACT FROM AUTHOR]

Sucralose stands as the most common non-nutritive sweetener; however, its metabolic effects have sparked significant controversy over the years. We aim to examine the effects of sucralose daily intake on glycemia, subjective appetite, and gut microbiota (GM) changes in subjects with overweight or obesity. In this randomized, crossover, and controlled trial, 23 participants with a body mass index between 25 kg/m2 and 39.9 kg/m2 will be assigned to one of two interventions to receive either sucralose (2 mg/kg/day equivalent to 40% of the acceptable daily intake) or glucose (control) for 4 weeks, each phase separated by a 4-week washout period. The glycemic response will be determined during a meal tolerance test, subjective appetite will be evaluated using a visual analog scale, and GM changes will be analyzed by next-generation sequencing of the bacterial rRNA 16S gene from fecal samples. All measures will be performed before and after intervention periods. We hypothesize that sucralose supplementation induces changes in glycemic response, subjective appetite, and gut microbiota in overweight and obese participants. This protocol was approved by the Ethics Committee of the UJAT (No. 0721) and was registered in the Australian New Zealand Clinical Trials Registry (ACTRN12621001531808). [ABSTRACT FROM AUTHOR]

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The present research investigated the physicochemical properties, phenolic profile, total phenolic content (TPC), total anthocyanin content (TAC), and antioxidant properties of cherry laurel (Prunus laurocerasus L.) pulp and marmalade (with sucrose and stevia addition) during storage. Pulp and marmalades were stored at room temperature and in the refrigerator and analyzed periodically. It was found that sample type, storage temperature, and time had a statistically significant effect on color parameters (L*, a*, b*, C*, and H°). Sample type also significantly affected pH, titratable acidity, total dry matter, ash, reducing sugar, sucrose, total sugar, TPC, TAC, and DPPH antioxidant activity. Storage time affected total dry matter, soluble solids content (SSC), reducing sugar, total sugar, TPC, TAC, and antioxidant activity, whereas storage temperature affected total dry matter, water-soluble dry matter, reducing sugar, total sugar, TPC, TAC, and antioxidant activity. Antioxidant activity, TPC, and TAC decreased with increasing storage time. Samples stored in the refrigerator displayed higher antioxidant activity, TPC, and TAC than those stored at room temperature. Pulp and marmalades contained chlorogenic acid and keracyanin chloride. Principal component analysis (PCA) was conducted to evaluate the impacts of sweetener type, storage time, and temperature. It was determined that sucrose-added marmalade formed a similar group, while pulp and stevia-added marmalade constituted a different group. Finally, this study supported the use of cherry laurel pulp and marmalade as natural sources of antioxidants. [ABSTRACT FROM AUTHOR]

Native to Argentina, Brazil and Paraguay, Stevia rebaudiana is a plant used as a sweetener and member of the Asteraceae family. In addition to having the potential to be a sweetener, stevia is a source of several nutrients that have a positive impact on human nutrition. The leaves of stevia include the chemical, rebaudioside, steviolbioside, and isosteviol and sweeter than sucrose with 0 calories. These steviol glycosides are valued commercially worldwide for their ability to replace sugar in food, drink, and nutraceuticals. They are also thought to have a sweet flavour. An overview of various extraction techniques, phytochemistry and commercial stevia applications is given in this article. a variety of goods, including drinks, bread goods, dairy products, and confections. Numerous researches demonstrate the potential health advantages of stevia against a range of illnesses, including the antimicrobial, anti-obesity, cancer prevention, anti-oxidant, anti-hypertensive and anti-diabetic qualities investigated in this work. According to clinical research, steviol glycosides--an important stevia phytochemical--are safe for ingestion by humans and do not cause either acute or serious toxic effects. This work potentially give an alternative perspective of stevia for the management of human ailments and contribute in creative stevia-based goods. [ABSTRACT FROM AUTHOR]