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Aim: To analyse the prevalence of post-traumatic stress disorder (PTSD) in nursing staff and the variables that may contribute to its development., Method: Cross-sectional study using a self-administered questionnaire given to nurses, nursing assistants and nursing supervisors in June 2020. It included sociodemographic, mental health, occupational, COVID-19 related variables, Modified Risk Perception Scale (modified RPS) score, Brief Resilience Scale (BRS) and Davidson Trauma Scale (DTS) score for the assessment of PTSD. Descriptive, bivariate, and multivariate analyses were performed., Results: Of the 344 participants, 88.7% were women and 93.6% cared for infected patients; 45.9% had PTSD (DTS≥40). The variables associated with PTSD were previous PTSD symptoms (OR=6.1, 95% CI [2.68-14.03]), death of a family member or friend due to COVID-19 (OR=2.3, 95% CI [1.22-4.39]), and higher scores on the modified RPS (OR= 1.1, 95% CI [1.07-1.31]). Higher BRS scores were associated with a lower risk of PTSD (OR=0.4, 95% CI [0.31-0.68])., Conclusions: The prevalence of PTSD in nursing staff is high, mainly in professionals with previous PTSD symptoms, family members or friends deceased from COVID-19, high risk perception and/or low resilience., (Copyright © 2021 Elsevier España, S.L.U. All rights reserved.)