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Plants are subjected to strong fluctuations in light intensity in their natural growth environment, caused both by unpredictable changes due to weather conditions and movement of clouds and upper canopy leaves and predictable changes during day-night cycle. The mechanisms of long-term acclimation to fluctuating light (FL) are still not well understood. Here, we used quantitative mass spectrometry to investigate long-term acclimation of low light-grown Arabidopsis thaliana to a FL condition that induces mild photooxidative stress. On the third day of exposure to FL, young and mature leaves were harvested in the morning and at the end of day for proteome analysis using a stable isotope labeling approach. We identified 2,313 proteins, out of which 559 proteins exhibited significant changes in abundance in at least one of the four experimental groups (morning-young, morning-mature, end-of-day-young, end-of-day-mature). A core set of 49 proteins showed significant responses to FL in three or four experimental groups, which included enhanced accumulation of proteins involved in photoprotection, cyclic electron flow around photosystem I, photorespiration, and glycolysis, while specific glutathione transferases and proteins involved in translation and chlorophyll biosynthesis were reduced in abundance. In addition, we observed pathway- and protein-specific changes predominantly at the end of day, whereas few changes were observed exclusively in the morning. Comparison of the proteome data with the matching transcript data revealed gene- and protein-specific responses, with several chloroplast-localized proteins decreasing in abundance despite increased gene expression under FL. Together, our data shows moderate but widespread alterations of protein abundance during acclimation to FL and suggests an important role of post-transcriptional regulation of protein abundance.

Changes in light intensity directly affect the performance of the photosynthetic apparatus. Light energy absorbed in excess of cells’ needs leads to production of reactive oxygen species and photo-oxidative damage. Excess light in both constant and dynamic environments induces photoprotective acclimation in plants. Distinct sets of signals and regulatory mechanisms are involved in acclimatory adjustment of photoprotection and photosynthesis under constant and dynamic (fluctuating) light conditions. We are still far away from drawing a comprehensive picture of acclimatory signal transduction pathways, particularly in dynamic environments. In this perspective article, we propose the use of Arabidopsis plants that produce H2O2 in chloroplasts (GO plants) under atmospheric CO2 levels as a tool to study the mechanisms of long-term acclimation to photo-oxidative stress. In our opinion there are new avenues to future investigations on acclimatory adjustments and signal transduction occurring in plants under dynamic light environments.