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Your search keyword '"V. E. Marquez"' showing total 2 results
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1. LAB-PEDIATRICS LABORATORY RESEARCH

Author

D. Carvalho, L. Bjerke, D. Bax, L. Chen, I. Kozarewa, S. Baker, R. Grundy, A. Ashworth, C. Lord, D. Hargrave, R. M. Reis, C. Jones, S. Bender, W. Feng, D. T. Jones, M. Kool, H. Cin, S. Pleier, S. Hutter, D. Sturm, H.-K. Liu, A. Korshunov, P. Lichter, S. M. Pfister, I. Alimova, D. K. Birks, P. Harris, S. Venkataraman, V. E. Marquez, N. K. Foreman, R. Vibhakar, S. Whiteway, A. Donson, E. Xipell, P. Jauregui, M. Gonzalez, s. tejada-solis, R. Diez-Valle, T. Tunon, I. Zazpe, J. Mora, A. M. Carcaboso, C. Gomez-MAnzano, J. Fueyo, M. Alonso, K. Dorris, M. Sobo, P. Holden, E. Panditharatna, S. Li, A. Margol, C. Stephenson, L. Miles, S. Goldman, S. Asgharzadeh, A. Onar, M. Fouladi, R. Drissi, A. Erdreich-Epstein, X. Ren, H. Zhou, K. Snyder, M. Stamper, J. Perez, J. Nazarian, T. Gershon, A. Crowther, I. Garcia, V. Gama, H. Yuan, S. Chang, M. Deshmukh, M. Hutt, W. Goldstein, A. Price, K. J. Lim, K. Warren, H. Chang, C. G. Eberhart, E. H. Raabe, K. Karakoula, K. P. Phipps, W. Harkness, R. Hayward, D. Thompson, T. S. Jacques, J. L. Darling, T. J. Warr, C. Guldal, C. Potts, R. Rotenberry, A. M. Kenney, V. Amani, A. M. Griesinger, A. M. Donson, L. T. Bemis, S. K. Schittone, M. Morgan, A. Thorburn, J. Mulcahy-Levy, I. Kolkowitz, N. Andor, T. Jensen, A. Banerjee, N. Gupta, C. Petritsch, M. Taylor, R. Hashizume, M. Tom, D. Haas-Kogan, S. Mueller, D. Stearns, N. Ma, R. Levy, D. Gate, J. Rodriguez, J. Breunig, M. Danielpour, and T. Town

Subjects
Cancer Research, medicine.medical_specialty, business.industry, 03 medical and health sciences, Abstracts, 0302 clinical medicine, Oncology, 030220 oncology & carcinogenesis, medicine, Medical physics, Neurology (clinical), Laboratory research, business, 030217 neurology & neurosurgery
Published
2012

2. Synthesis, stability, and protonation studies of a self-complementary dodecamer containing the modified nucleoside 2'-deoxyzebularine.

Author

M. Vives, R. Eritja, R. Tauler, V. E. Marquez, and R. Gargallo

Subjects
NUCLEOSIDES, AMMONIA, URACIL, PYRIMIDINE nucleotides, ACID-base chemistry
Abstract

The nucleoside 2'-deoxyzebularine (K) was incorporated into the self-complementary dodecamer 5'-CGTACGKGTACG-3' by solid-phase 2-cyanoethylphosphoramidite chemistry using dimethoxytrityl (DMT) as the 5'-hydroxyl protecting group. Standard synthesis cycles using trichloroacetic acid and short ammonia treatment (50°C for 30 min) were found to be the optimal conditions to obtain the desired dodecamer with minimum acid and basic degradation of the acid- and base-sensitive 2-pyrimidinone residue. The protonation equilibria of the K nucleoside and of the dodecamer at 37°C were studied by means of spectroscopically monitored titrations. For the K nucleoside, a pKa value of 3.13 ± 0.09 was obtained. For the dodecamer, four acid-base species were found in the pH range 2–12, with pKa values of 9.60 ± 0.07, 4.46 ± 0.16, and 2.87 ± 0.19. Melting experiments were carried out to confirm the proposed acid-base concentration profiles. Finally, kinetic experiments were also carried out at several pH values to evaluate the stability of the K nucleoside and of the dodecamer. An increased stability was shown by the K nucleoside when incorporated into the dodecamer. Multivariate methods based on both hard- and soft-modeling were applied for the analysis of spectroscopic data, allowing the estimation of concentration profiles and pure spectra. © 2003 Wiley Periodicals, Inc. Biopolymers 70: 27–43, 2004 [ABSTRACT FROM AUTHOR]

Published
2004
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