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9 results on '"Walichnowski A"'

6. Simple sequence repeat marker development from bacterial artificial chromosome end sequences and expressed sequence tags of flax (Linum usitatissimum L.)

Author

Sylvie Cloutier, Natasa Radovanovic, Raju Datla, Evelyn Miranda, Andrzej Z. Walichnowski, Scott Duguid, Elsa Reimer, Raja Ragupathy, Gordon Rowland, and Kerry Ward

Subjects
Genetic Markers, Chromosomes, Artificial, Bacterial, Linum, comparative analysis, oil seed crops, Genotype, trinucleotides, Biology, quantitative trait locus, bacterial artificial chromosomes, Flax, Genetics, genomics, whole-genome shotgun, Nucleotide Motifs, Association mapping, Genome size, expressed sequence tags, simple sequence-repeat markers, Original Paper, Expressed sequence tag, Base Sequence, Nucleotides, Shotgun sequencing, food and beverages, limun usitatissimum, General Medicine, genome sizes, biology.organism_classification, genetic resources, Dinucleotides, Genetic Techniques, Genetic marker, Microsatellite, Agronomy and Crop Science, simple sequence repeats, Microsatellite Repeats, Biotechnology, Reference genome
Abstract

Flax is an important oilseed crop in North America and is mostly grown as a fibre crop in Europe. As a self-pollinated diploid with a small estimated genome size of ~370 Mb, flax is well suited for fast progress in genomics. In the last few years, important genetic resources have been developed for this crop. Here, we describe the assessment and comparative analyses of 1,506 putative simple sequence repeats (SSRs) of which, 1,164 were derived from BAC-end sequences (BESs) and 342 from expressed sequence tags (ESTs). The SSRs were assessed on a panel of 16 flax accessions with 673 (58 %) and 145 (42 %) primer pairs being polymorphic in the BESs and ESTs, respectively. With 818 novel polymorphic SSR primer pairs reported in this study, the repertoire of available SSRs in flax has more than doubled from the combined total of 508 of all previous reports. Among nucleotide motifs, trinucleotides were the most abundant irrespective of the class, but dinucleotides were the most polymorphic. SSR length was also positively correlated with polymorphism. Two dinucleotide (AT/TA and AG/GA) and two trinucleotide (AAT/ATA/TAA and GAA/AGA/AAG) motifs and their iterations, different from those reported in many other crops, accounted for more than half of all the SSRs and were also more polymorphic (63.4 %) than the rest of the markers (42.7 %). This improved resource promises to be useful in genetic, quantitative trait loci (QTL) and association mapping as well as for anchoring the physical/genetic map with the whole genome shotgun reference sequence of flax. Electronic supplementary material The online version of this article (doi:10.1007/s00122-012-1860-4) contains supplementary material, which is available to authorized users.

Published
2012
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7. Determination of oxygen tension in the subcutaneous tissue of cosmonauts during the Salyut-6 mission

Author

Baranski, S, Bloszczynski, R, Hermaszewski, M, Kubiczkowa, J, Piorko, A, Saganiak, R, Sarol, Z, Skibniewsky, F, Stendera, J, and Walichnowski, W

Subjects
Aerospace Medicine
Abstract

A polarographic technique was used to measure the oxygen tension in subcutaneous tissue of the forearm of a cosmonaut prior to, after, and on the fourth day of a space mission performed by Salut-6. A drop in the oxygen exchange rate in the peripheral tissues during weightlessness was observed. The mechanisms of this change are studied, taking into consideration the blood distribution in the organism and microcirculation disorders reflected by a decreased blood flow rate in arterial-venous junctions.

Published
1982

8. Investigation of cooling properties of the gaseous medium of a space station

Author

Baranski, S, Blosznyski, R, Hermaszewski, M, Kubiczkowa, J, Piorko, A, Saganiak, R, Sarol, Z, Skibniewski, F, Stendera, J, and Walichnowski, W

Subjects
Spacecraft Design, Testing And Performance
Abstract

An investigation of cooling properties of the gaseous medium was performed in the biosatellite Kosmos-936 as well as in the orbital complexes Soyuz-28/Salyut-6 and Soyuz-30/Salyut-6 with the aid of an especially constructed electric dynamic catathermometer. In this instrument current was measured which was necessary to keep a steady settled temperature of the sensing device. The investigation was performed because of the disturbed heat exhange of the human body caused by lack of natural convection in weightlessness. The instrument also enabled objective estimation of the temperature of the cosmonaut's ody in six optionally selected regions. The results obtained by means of the catathermometer will also enable defining the appropriate hygienic conditions of the gaseous medium of space stations.

Published
1982

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