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Modern geodetic technologies, including high-precision ground-based gravity measurements, satellite gravimetry, satellite altimetry, Global Navigation Satellite Systems (GNSS), and Interferometric Synthetic Aperture Radar(InSAR), offer a wealth of observations for monitoring global hydrological processes with exceptional accuracy and spatio-temporal resolutions. Mass redistribution and Earth’s surface deformation over land related to global and regional water cycling can be inferred from modern gravimetry, altimetry, GNSS, and InSAR techniques. Hydrogeodesy becomes an emerging field of geodesy aiming to analyze the changes of water in the Earth system. The paper introduces the China’s advances in hydrogeodesy in recent years. It brings together multiple geodetic teams’ work from China, showcasing the application of modern geodetic technologies in the field of hydrology, including research on terrestrial water storage, groundwater storage, glaciers/ice sheets, and reservoir water storage.

Objective: This study aimed to find the best clarifying method of fermented wine by using L. lucidus Turcz and improve its quality and stability. Methods: The gelatin clarification method, bentonite clarification method, chitosan clarification method, heat treatment method, membrane filtration method and static clarification at different temperatures were used to treat the wine fermented with L. lucidus Turcz. As an evaluation index for the clarification effect, the best clarification method and related parameters were selected by combining turbidity, color, light transmittance, the content of alcohol, total acid, total sugar, caffeic acid and rosmarinic acid, and sensory scores. Results: The results showed that, after 70 ℃ water bath heat treatment for 20 min, the wine fermented with L. lucidus had the best clarity, with a turbidity of only 1.19 EBC and a light transmittance of 95.8%. The highest sensory score was 88.9 points, and the soluble solid content was 8.02%. The content of alcohol, total acid, total sugar, caffeic acid and rosmarinic acid had little change after the clarification. Moreover, the fermented wine in the present study had no turbidity during storage and showed good stability after heat treatment. Conclusion: After heat treatment, the fermented wine of L. lucidus Turcz is orange-red, transparent, odorless, good taste, and stable quality.

BackgroundHeart failure (HF) with preserved ejection fraction (HFpEF) is a growing public health burden, with mortality and rehospitalization rates comparable to HF with reduced ejection fraction (HFrEF). The evidence for the clinical usefulness of soluble suppression of tumorigenicity 2 (sST2) in HFpEF is contradictory. Therefore, we conducted the following systematic review and meta-analysis to assess the diagnostic and prognostic value of serum sST2 in HFpEF.MethodsPubMed and Scopus were searched exhaustively from their inception until March 15, 2022. In diagnostic analysis, we compared the diagnostic value of serum sST2 in HFpEF to NT pro-BNP. We separately pooled the unadjusted and multivariate-adjusted hazard ratios (HRs) and the corresponding 95% confidence intervals (CIs) in prognostic analysis.ResultsA total of 16 publications from 2008 to 2021 were examined. The results of this analysis were as follow: Firstly, compared with NT pro-BNP, sST2 obtains poor diagnostic performance in independently identifying HFpEF from healthy controls, hypertensive patients, and HFrEF patient. Nevertheless, it may provide incremental value to other biomarkers for diagnosing HFpEF and deserves further investigation. Secondly, log sST2 was independently associated with adverse endpoints on multivariable analysis after adjusting for variables such as age, sex, race, and NYHA class. Per log unit rise in sST2, there was a 2.76-fold increased risk of all-cause death [HR:2.76; 95% CI (1.24, 6.16); p = 0.516, I2 = 0%; P = 0.013] and a 6.52-fold increased risk in the composite endpoint of all-cause death and HF hospitalization [HR:6.52; 95% CI (2.34, 18.19); p = 0.985, I2 = 0%; P = 0.000]. Finally, the optimal threshold levels of serum sST2 need further determined.ConclusionsHigher sST2 was strongly linked to an increased risk of adverse outcomes in HFpEE. Especially, log sST2 independently predicted all-cause death and the composite endpoint of all-cause death and HF hospitalization. However, prospective and multicenter studies with large-sample and extended follow-up periods are required to validate our results due to limitations in our research.

BackgroundHeart failure with preserved ejection fraction (HFpEF) is an increasing public health concern. Currently, data regarding the clinical application value of plasma Galectin-3 (Gal-3) in HFpEF are contradictory. Therefore, we performed the following meta-analysis to appraise the clinical implications of serum Gal-3 in HFpEF, including its capacity to predict new-onset disease, long-term unfavorable endpoints, and the degree of cardiac structural abnormality and left ventricular diastolic dysfunction (LVDD).MethodsPubMed, Embase, Scopus, and Web of Science were retrieved exhaustively from their inception until November 30, 2021, to obtain studies assessing the correlation between plasma Gal-3 and the clinical features of HFpEF (new-onset HFpEF, adverse outcomes, and echocardiographic parameters related to abnormal cardiac structure and LVDD).ResultsA total of 24 papers containing 27 studies were ultimately included in the present research. The results of the meta-analysis revealed that high plasma Gal-3 levels are strongly associated with the following clinical characteristics of HFpEF: (i) the increased risk of new-onset HFpEF (HR: 1.11; 95% CI: 1.04-1.19; p = 0.910, I2 = 0%; P = 0.002); (ii) the high risk of adverse outcomes of HFpEF patients [all-cause death (HR: 1.55; 95% CI: 1.27-1.87; p = 0.138, I2 = 42%; P = 0.000) and the composite events [all-cause death and HF hospitalization (HR: 1.50; 95% CI: 1.30-1.74; p = 0.001, I2 = 61%; P = 0.000) or cardiovascular (CV) death and HF hospitalization (HR: 1.71; 95% CI: 1.51-1.94; p = 0.036, I2 = 58%; P = 0.000)]; (iii) echocardiographic indices [E/e ratio (r: 0.425, 95% CI: 0.184-0.617; p = 0.000, I2 = 93%; P = 0.001) and DT (r: 0.502, 95% CI: 0.061-0.779; p = 0.001 I2 = 91%; P = 0.027)].ConclusionsPlasma Gal-3 might be employed as an additional predictor for new-onset HFpEF, the adverse prognosis in HFpEF patients (all-cause death, the composite endpoints of all-cause death and HF hospitalization or CV death and HF hospitalization), and the severity of LVDD in HFpEF populations.

The effect of weak intercalated layers on the seismic response of rock slopes is investigated. Dozens of rock slopes containing weak intercalated layers are modeled in FLAC. The geometric property of the weak intercalated layer has a significant effect on the seismic slope response. The responses are generally greater as the thickness of the weak intercalated layer increases. The slope case with a 10-m-thickness and 20°-dip-angle weak intercalated layer yields the strongest dynamic response. A predictive model for the amplification of peak ground acceleration is developed, which can be used to estimate the acceleration amplifications for weak-layer-containing rock slopes. [ABSTRACT FROM AUTHOR]

Calcium (Ca) and magnesium (Mg) in drinking water and the relevant health effects have been ignored for too long. This study aims to reveal the concentrations, spatial distributions, origins and contributions to the daily estimated average requirements of Ca and Mg in public drinking water. Using hydrochemical data of collected samples of public drinking water in 314 cities across China, the contributions of Ca and Mg intakes from public drinking water to their daily estimated average requirements (EARs) were assessed. And the significance of Ca/Mg ratio and total hardness (TH) was evaluated as well. The Ca and Mg concentrations of the samples were in the range of 2.5–155.1 mg/L and 0.2–81.9 mg/L, with an average of 40.4 mg/L and 12.4 mg/L, respectively. There exist obvious differences in Ca and Mg concentrations in different regions, under the impact of climate conditions and water–rock interactions. The intake of Ca via the consumption of public drinking water for adults may be twice as much as that for other age groups. In cities with high Ca levels in public drinking water, the Ca contributions to EAR could reach up to 51.59% for adults. By contrast, Mg in drinking water is an important and even the main pathway to ingest Mg for infants and children. Therefore, public drinking water is critical for Ca and Mg intake among urban residents of China. Besides, attention should be paid to the health effects of high Ca/Mg ratio and low TH in public drinking water, especially in southern China. This research is the first systematic and comprehensive national scale study of Ca and Mg in public drinking water and can provide an important reference to improve healthy public drinking water standards around the world. [ABSTRACT FROM AUTHOR]

Myocardial fibrosis is a critical factor in the development of heart failure with preserved ejection fraction (HFpEF). Linggui Qihua decoction (LGQHD) is an experienced formula, which has been proven to be effective on HFpEF in clinical and in experiments. Objective. This study aimed to observe the effect of LGQHD on HFpEF and its underlying mechanism. Methods. Spontaneously hypertensive rats (SHR) were induced with high-glucose and high-fat to establish HFpEF models and were treated with LGQHD for 8 weeks. The heart structure was detected by echocardiography, and the histopathological changes of the myocardium were observed by hematoxylin-eosin (HE) and Masson staining. Reverse transcription PCR (RT-PCR) and western blot were used to detect mRNA and protein expression of the target gene in rat myocardium. Results. In this study, LGQHD improved cardiac morphology and atrial fibrosis in HfpEF rats, decreased tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA expression, up-regulated matrix metalloproteinase-9 (MMP-9) mRNA expression, and inhibited the expression of angiotensin II (Ang II), angiotensin II type 1 receptor (AT1), transforming growth factor β1 (TGF-β1), Smad2/3 mRNA, and protein in myocardial tissue of HFpEF rats. Conclusion. LGQHD can suppress atrial fibrosis in HFpEF by modulating the TGF-β1/Smad2/3 pathway. [ABSTRACT FROM AUTHOR]

Two polysaccharides from Carthamus tinctorius L. (CTLP-1 and CTLP-2) were purified, and their structures were analyzed by physical and chemical testing. CTLP-1 had a mass of 5900 Da that was composed of arabinose, glucose, and galactose with a mass molar ratio of 6.7:4.2:1. The backbone of CTLP-1 was →1)-α-GalAp-(1→4)-α-Arap-(1→2)-α-Glup-(4→. CTLP-2 had a mass of 8200 Da that was composed of arabinose, glucose, and galactose with a mass molar ratio of 16.76:4.28:1. The backbone of CTLP-2 was →1)-α-Galp-(2,6 →1)-α-Arap-(4,6 →1)-α-Glup-(3→. Both of them exhibited a high reducing power, hydroxyl radical scavenging activity, DPPH radical scavenging activity and ABTS radical scavenging activity, moderate Fe2+ chelating activity and superoxide anion scavenging activity, implying that they might be potential antioxidants. [ABSTRACT FROM AUTHOR]

Objective: Current pharmacological intervention for the cancer-related pain is still limited. The aim of this study was to explore whether repetitive transcranial magnetic stimulation (rTMS) could be an effective adjuvant therapy to reduce pain in patients with advanced non-small cell lung cancer (NSCLC). Methods: This was a randomized, sham–controlled study. A total of 41 advanced NSCLC patients with uncontrolled pain (score≥4 on pain intensity assessed with an 11-point numeric rating scale) were randomized to receive active (10 Hz, 2000 stimuli) (n = 20) or sham rTMS (n = 20) for 3 weeks. Pain was the primary outcome and was assessed with the Numeric Rating Scale (NRS). Secondary outcomes were oral morphine equivalent (OME) daily dose, quality of life (WHO Quality of Life-BREF), and psychological distress (the Hospital Depression and Anxiety Scale). All outcomes were measured at baseline, 3 days, 1 week, 2 weeks, and 3 weeks. Results: The pain intensity in both groups decreased gradually from day 3 and decreased to the lowest at the week 3, with a decrease rate of 41.09% in the rTMS group and 23.23% in the sham group. The NRS score of the rTMS group was significantly lower than that of the sham group on the week 2 (p < 0.001, Cohen's d =1.135) and week 3 (p =0.017, Cohen's d = -0.822). The OME daily dose, physiology and psychology domains of WHOQOL-BREF scores, as well as the HAM-A and HAM-D scores all were significantly improved at week 3 in rTMS group. Conclusion: Advanced NSCL patients with cancer pain treated with rTMS showed better greater pain relief, lower dosage of opioid, and better mood states and quality of life. rTMS is expected to be a new effective adjuvant therapy for cancer pain in advanced NSCLC patients. [ABSTRACT FROM AUTHOR]

During anther development, the transformation of the microspore into mature pollen occurs under the protection of first the tetrad wall and later the pollen wall. Mutations in genes involved in this wall transition often lead to microspore rupture and male sterility; some such mutants, such as the reversible male sterile (rvms) mutant, are thermo/photoperiod-sensitive genic male sterile (P/TGMS) lines. Previous studies have shown that slow development is a general mechanism of P/TGMS fertility restoration. In this study, we identified restorer of rvms-2 (res2), which is an allele of QUARTET 3 (QRT3) encoding a polygalacturonase that shows delayed degradation of the tetrad pectin wall. We found that MS188, a tapetum-specific transcription factor essential for pollen wall formation, can activate QRT3 expression for pectin wall degradation, indicating a non-cell-autonomous pathway involved in the regulation of the cell wall transition. Further assays showed that a delay in degradation of the tetrad pectin wall is responsible for the fertility restoration of rvms and other P/TGMS lines, whereas early expression of QRT3 eliminates low temperature restoration of rvms-2 fertility. Taken together, these results suggest a likely cellular mechanism of fertility restoration in P/TGMS lines, that is, slow development during the cell wall transition of P/TGMS microspores may reduce the requirement for their wall protection and thus support their development into functional pollens, leading to restored fertility. This work identified a restorer (res2) of the P/TGMS mutant rvms-2 ; res2 is an allele of QRT3 , which encodes a polygalacturonase that participates in tetrad pectin wall degradation. The authors showed that a delay in degradation of the tetrad pectin wall is responsible for fertility restoration of rvms and other P/TGMS lines, revealing a likely cellular mechanism of fertility restoration in P/TGMS lines. [ABSTRACT FROM AUTHOR]

Rainfall represents one of the most significant cause of landslide hazards. With runoff in the saturated zone of shallow soil considered, an improved Green–Ampt model was proposed to depict the rainfall infiltration process. Furthermore, a formula for calculating the depth of wetting front was derived, and the slope stability was evaluated accordingly. Finally, a formula for calculating the safety factor of multilayer slopes above different surfaces was obtained via the limit equilibrium method. An illustration is a multilayer colluvial slope in Guangdong Province of China, the results indicated that the downward speed of wetting front decreases gradually within the same layer with continuous rainfall but increases significantly when it extends to the interfaces between the soil layers. Safety factor of the slope decreases sharply at the initial stage of rainfall but then more slowly. Slope failure is more likely to occur along the first interface between the soil layers than along the surface of wetting front. The result from the proposed model is closer to the result of the physical model test than from the existed Green–Ampt model. The improved Green–Ampt model for multilayer slopes can accurately evaluate the infiltration process and related stability of multilayer slopes under rainfall. [ABSTRACT FROM AUTHOR]

PANX2 forms large-pore channels mediating ATP release in response to physiological and pathological stimuli. Although PANX2 shows involvements in glioma genesis, the underlying mechanism remains unclear. PANX2 mRNA expression was analyzed via Oncomine and was confirmed via Gene Expression Profiling Interactive Analysis (GEPIA). The influence of PANX2 on overall survival (OS) of glioma was evaluated using LinkedOmics and further assessed through Cox regression analysis. The correlated genes with PANX2 acquired from LinkedOmics were validated through GEPIA and cBioPortal. Protein-protein interaction (PPI) of these genes was then obtained using Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape with MCODE plug-in. All the PANX2-related genes underwent Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The correlation between PANX2 and cancer immune infiltrates was evaluated via Tumor Immune Estimation Resource (TIMER). A higher expression of PANX2 only revealed a better OS in brain low grade glioma (LGG). PANX2-related genes in LGG functionally enriched in neuroactive ligand-receptor interaction, synaptic vesicle cycle, and calcium signaling. The hub genes from highest module of PPI were mainly linked to chemical synaptic transmission, plasma membrane, neuropeptide, and the pathway of neuroactive ligand-receptor interaction. Besides, PANX2 expression was negatively associated with infiltrating levels of macrophage, dendritic cells, and CD4+ T cells. This study demonstrated that PANX2 likely participated in LGG pathogenesis by affecting multiple molecular pathways and immune-related processes. PANX2 was associated with LGG prognosis and might become a promising therapeutic target of LGG. [ABSTRACT FROM AUTHOR]

Purpose: This study aimed to explore the key molecular pathways involved in Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) and thereby identify hub genes to be potentially used as novel biomarkers using a bioinformatics approach. Methods: Raw GSE109178 data were collected from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was conducted on the top 50% of altered genes. The key modules associated with the clinical features of DMD and BMD were identified. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the DAVID website. A protein-protein interaction (PPI) network was constructed using the STRING website. MCODE, together with the Cytohubba plug-ins of Cytoscape, screened out the potential hub genes, which were subsequently verified via receiver operating characteristic (ROC) curves in other datasets. Results: Among the 11 modules obtained, the black module was predominantly associated with pathology and DMD, whereas the light-green module was primarily related to age and BMD. Functional enrichment assessments indicated that the genes in the black module were primarily clustered in "immune response" and "phagosome," whereas the ones in the light-green module were chiefly enriched in "protein polyubiquitination". Eleven essential genes were eventually identified, including VCAM1, TYROBP, CD44, ITGB2, CSF1R, LCP2, C3AR1, CCL2, and ITGAM for DMD, along with UBA5 and UBR2 for BMD. Conclusion: Overall, our findings may be useful for investigating the mechanisms underlying DMD and BMD. In addition, the hub genes discovered might serve as novel molecular markers correlated with dystrophinopathies. [ABSTRACT FROM AUTHOR]

Sporophytic pollen coat proteins (sPCPs) derived from the anther tapetum are deposited into pollen wall cavities and function in pollen–stigma interactions, pollen hydration, and environmental protection. In Arabidopsis, 13 highly abundant proteins have been identified in pollen coat, including seven major glycine-rich proteins GRP14, 16, 17, 18, 19, 20, and GRP–oleosin; two caleosin-related family proteins (AT1G23240 and AT1G23250); three lipase proteins EXL4, EXL5 and EXL6, and ATA27/BGLU20. Here, we show that GRP14, 17, 18, 19, and EXL4 and EXL6 fused with green fluorescent protein (GFP) are translated in the tapetum and then accumulate in the anther locule following tapetum degeneration. The expression of these sPCPs is dependent on two essential tapetum transcription factors, MALE STERILE188 (MS188) and MALE STERILITY 1 (MS1). The majority of sPCP genes are up-regulated within 30 h after MS1 induction and could be restored by MS1 expression driven by the MS188 promoter in ms188 , indicating that MS1 is sufficient to activate their expression; however, additional MS1 downstream factors appear to be required for high-level sPCP expression. Our ChIP, in vivo transactivation assay, and EMSA data indicate that MS188 directly activates MS1. Together, these results reveal a regulatory cascade whereby outer pollen wall formation is regulated by MS188 followed by synthesis of sPCPs controlled by MS1. [ABSTRACT FROM AUTHOR]

Pollen wall consists of several complex layers which form elaborate species-specific patterns. In Arabidopsis, the transcription factor ABORTED MICROSPORE (AMS) is a master regulator of exine formation, and another transcription factor, TRANSPOSABLE ELEMENT SILENCING VIA AT-HOOK (TEK), specifies formation of the nexine layer. However, knowledge regarding the temporal regulatory roles of TEK in pollen wall development is limited. Here, TEK-GFP driven by the AMS promoter was prematurely expressed in the tapetal nuclei, leading to complete male sterility in the pAMS:TEK-GFP (pat) transgenic lines with the wild-type background. Cytological observations in the pat anthers showed impaired callose synthesis and aberrant exine patterning. CALLOSE SYNTHASE5 (CalS5) is required for callose synthesis, and expression of CalS5 in pat plants was significantly reduced. We demonstrated that TEK negatively regulates CalS5 expression after the tetrad stage in wild-type anthers and further discovered that premature TEK-GFP in pat directly represses CalS5 expression through histone modification. Our findings show that TEK flexibly mediates its different functions via different temporal regulation, revealing that the temporal regulation of TEK is essential for exine patterning. Moreover, the result that the repression of CalS5 by TEK after the tetrad stage coincides with the timing of callose wall dissolution suggests that tapetum utilizes temporal regulation of genes to stop callose wall synthesis, which, together with the activation of callase activity, achieves microspore release and pollen wall patterning. Author summary: To develop into mature pollen grains, microspores require formation of the pollen wall. To date, pollen wall developmental events, including production and transportation of pollen wall components, synthesis and degradation of the callose wall, and deposition and demixing of primexine, have been studied in Arabidopsis, and a number of anther- or tapetum-specific genes involved in pollen wall formation have been uncovered. However, whether the specific expression patterns of these genes contribute to pollen wall development or patterning remains unclear. Here, we show that TEK, a transcription factor that specifies formation of nexine (the inner layer of the pollen wall exine), represses the expression of the callose synthase CalS5 after the tetrad stage, which accurately fits with the timing of callose wall dissolution causing microspore release. Moreover, we show that premature expression of TEK in the wild-type anthers disturbs callose wall synthesis and pollen wall patterning. This work reveals that a pollen wall regulator must be kept under a strict temporal control to perform its functions, and that these temporal controls are coordinated with other pollen wall developmental events to determine pollen wall formation and patterning. [ABSTRACT FROM AUTHOR]

The sexine layer of pollen grain is mainly composed of sporopollenins. The sporophytic secretory tapetum is required for the biosynthesis of sporopollenin. Although several enzymes involved in sporopollenin biosynthesis have been reported, the regulatory mechanism of these enzymes in tapetal layer remains elusive. ABORTED MICROSPORES ( AMS) and MALE STERILE 188/ MYB103/ MYB80 ( MS188/ MYB103/ MYB80) are two tapetal cell-specific transcription factors required for pollen wall formation. AMS functions upstream of MS188. Here we report that AMS and MS188 target the CYP703A2 gene, which is involved in sporopollenin biosynthesis. We found that AMS and MS188 were localized in tapetum while CYP703A2 was localized in both tapetum and locule. Chromatin immunoprecipitation (Ch IP) showed that MS188 directly bound to the promoter of CYP703A2 and luciferase-inducible assay showed that MS188 activated the expression of CYP703A2. Yeast two-hybrid and electrophoretic mobility shift assays ( EMSAs) further demonstrated that MS188 complexed with AMS. The expression of CYP703A2 could be partially restored by the elevated levels of MS188 in the ams mutant. Therefore, our data reveal that MS188 coordinates with AMS to activate CYP703A2 in sporopollenin biosynthesis of plant tapetum. [ABSTRACT FROM AUTHOR]

This study aimed at determining the effects of purified chondroitin sulfate (ChS) from pig laryngeal cartilage on the lipid and antioxidant metabolisms of male Sprague-Dawley rats that are fed a high-fat diet. Thirty-two male rats were divided into four groups and fed for 5 weeks on a standard diet, a high-fat diet or a high-fat diet plus ChS. It was demonstrated that the high-fat diet provoked lipid peroxidation and induced a severe depletion of lipoprotein lipase, hepatic lipidase, glutathione, catalase and superoxide dismutase levels. ChS was effective in reducing triglycerides, total cholesterol, low-density-lipid cholesterol and malondialdehyde levels elevated by the high-fat diet. In addition, ChS might reduce the risk of atherosclerosis, as high-density-lipid cholesterol, lipoprotein lipase, hepatic lipidase and the ratio of high-density-lipoprotein cholesterol to total cholesterol were significantly higher than in the high-fat-diet rats. ChS restored the endogenous antioxidants glutathione, catalase and superoxide dismutase. These results showed that ChS was potent in lipid-lowering and altering the antioxidative enzyme; however, excess ChS would disturb lipid profiles that went beyond the normal limits. [ABSTRACT FROM AUTHOR]

This study compared the free radical-scavenging properties of chondroitin sulfate (ChS) from pig laryngeal cartilage and its reduced or sulfonated derivatives. The binding behavior between Cu 2+ and ChS and its derivatives, and the interaction between superoxide radical and ChS were studied by fluorescence quenching, equilibrium dialysis, infrared spectra and thermal analysis. Purified ChS inhibited the generation of hydroxyl radical and scavenged superoxide radical in a concentration-dependent manner. Reduced ChS did not scavenge hydroxyl radical and superoxide radical. Sulfonated ChS had no hydroxyl radical scavenging activity but scavenged superoxide radical as strongly as purified ChS. ChS showed strong binding activity with Cu 2+ in deionized water but not in 0.01-M HCl. Both reduced ChS and sulfonated ChS did not exhibit such chelating behavior. The structural basis of hydroxyl radical inhibiting of ChS was attributed to a complex of the Cu 2+ with the carboxyl group of glucuronic acid residue. The reaction of superoxide radical with the sulfate ester and the carboxyl group may be the basis of superoxide radical scavenging activity of ChS. [ABSTRACT FROM AUTHOR]

Plant growth and mineral element accumulation in Brassica juncea var. crispifolia (crisped-leaf mustard) under exposure to lanthanum (La) and cadmium (Cd) were studied by employing a hydroponic experiment with a complete two-factorial design. Four levels of La (0.05–5.0 mg L -1 ) and two levels of Cd (1.0 and 10.0 mg L -1 ) were used in this experiment. Lanthanum did not improve plant growth in this experiment. Addition of La (≥ 1.0 mg L -1 ) or Cd (≥ 10 mg L -1 ) to the solution inhibited root elongation. Lanthanum treatments reduced accumulations of iron (Fe), manganese (Mn), and zinc (Zn) in roots, and Mn in shoots. Lanthanum at ≥ 1.0 mg L -1 limited the Cd translocation from roots to shoots and thus decreased the accumulation of Cd in shoots. Cadmium had no influence on La accumulations in roots, but inhibited the accumulation of La in shoots. The study results suggest that applications of rare earth elements in vegetables would be potentially risky to human health. [ABSTRACT FROM AUTHOR]

Rugao city is a typical longevity area taking shallow groundwater as the primary drinking water source. To determine the relationship between longevity and groundwater conditions, the hydrogeochemical characteristics and related causes of potable groundwater were investigated. On this basis, the water quality index (WQI) and hazard index (HI) of groundwater were evaluated. Meanwhile, the nutrient indicators beneficial to human health, like Ca and Mg concentrations, were also considered to explore the relationship. The results were as following: (1) 91.3% of water samples fell under the Ca/Mg–HCO 3 water type, which resulted from the dissolution of silicate rock. Na, Cl-, Br, B in groundwater emanated from seawater intrusion. The abnormal concentrations of NO 3 − and As also indicated that anthropogenic activities had exerted significant influences on groundwater quality. (2) The average WQI value was 30.19, which meant that the overall groundwater quality in Rugao city was pretty good. However, 8 water samples were found to have HI values above 1, which might be attributed to the high concentration of As (maximum value 0.0407 mg/L; mean value 0.0076 mg/L). In general, low WQI and HI values corresponded to towns with a high longevity population; what's more, WQI and HI values of Rugao city were lower than those of non-longevity areas. (3) Comparing with adjacent non-longevity areas, the potable groundwater in Rugao city had the characteristics of high Ca (mean value 123.57 mg/L), high Mg (mean value 50.33 mg/L) and high SO 4 2- (mean value 525.19 mg/L). The daily intake of Ca and Mg from drinking water could meet 12.4% and 22.4% of daily Ca and Mg requirements, respectively. Also, the areas where the Sr and B concentrations were higher usually had higher life expectancy. The high concentrations of Ca, Mg, SO 4 2-, Sr and B in drinking water, as well as low WQI and HI values, probably contribute to physical health and longevity. This research helps provide an insight into the relationship between groundwater quality and health and can serve as a reference for drinking water quality management. [Display omitted] • Water-rock interaction and seawater intrusion dictate groundwater composition. • Anthropogenic activities have affected groundwater quality. • Towns in Rugao city with high longevity usually have low WQI and HI values. • The potable groundwater in Rugao city has high levels of nutrient elements. [ABSTRACT FROM AUTHOR]

Highlights • The plant polyketide synthase is required for the sporopollenin precursor biosynthesis. • Knockout of the rice OsPKS1 leads to abnormal bacula deposition and ubisch body structures for sexine formation. • This protein was preferentially localized to the ER in tapetal cells during microspore development. • The function of polyketide synthase in sporopollenin synthesis pathway are conserved in rice and Arabidopsis. Abstract The sporopollenin precursors, as a general constituent of sexine, are synthesized in the tapetum and deposited on the pollen surface after transportation and processing. The polyketide synthase condenses the acyl-CoA into a hydroxyalkyl α-pyrone, which is predicted to be a component of the sporopollenin precursors. In this study, we found that the rice POLYKETIDE SYNTHASE 1 (OsPKS1) was the orthologue of Arabidopsis POLYKETIDE SYNTHASE A/LESS ADHESIVE POLLEN 6 (PKSA/LAP6) through sequence alignment. The OsPKS1 knockout mutants obtained by Crispr-Cas9-mediated editing exhibited a complete male sterile phenotype. Cytological observations revealed that abnormal bacula deposition and ubisch body structures for sexine formation led to pollen rupture in ospks1. The expression analysis showed that the OsPKS1 was highly expressed in tapetal cells and anther locules from stage 9 to stage 11 during anther development in rice. Subcellular localization demonstrated that the OsPKS1 protein was preferentially localized to the ER. The genomic sequence of OsPKS1 driven by the PKSA/LAP6 promoter restored the sexine pattern of Arabidopsis pksa/lap6. These results indicated that OsPKS1 is required for sexine layer formation in rice and functionally conserved in the sporopollenin synthesis pathway. [ABSTRACT FROM AUTHOR]

Abstract: In case hazardous contaminants are suddenly released indoors, the prompt and proper emergency responses are critical to protect occupants. This paper aims to provide a framework for determining the optimal combination of ventilation and evacuation strategies by considering the uncertainty of source locations. The certainty of source locations is classified as complete certainty, incomplete certainty, and complete uncertainty to cover all the possible situations. According to this classification, three types of decision analysis models are presented. A new concept, efficiency factor of contaminant source (EFCS), is incorporated in these models to evaluate the payoffs of the ventilation and evacuation strategies. A procedure of decision-making based on these models is proposed and demonstrated by numerical studies of one hundred scenarios with ten ventilation modes, two evacuation modes, and five source locations. The results show that the models can be useful to direct the decision analysis of both the ventilation and evacuation strategies. In addition, the certainty of the source locations has an important effect on the outcomes of the decision-making. [Copyright &y& Elsevier]

Illicium simonsii Maxim (1888) is a medicinal species of the genus Illicium in the Illiciaceae family. It is commonly used to cure gastro-frigid vomiting, cystic hernia, gas pains in the chest, and scabies as folk medicine. To utilize its resources efficiently, the complete chloroplast genome of I. simonsii was sequenced, assembled, and annotated by using high-throughput sequencing data. The complete chloroplast genome was 143,038 bp in length, with a large single-copy region (LSC) of 101,094 bp, a short single-copy region (SSC) of 20,070 bp, and a pair of inverted repeats (IRs) of 21,874 bp. A total of 113 genes were annotated, including 79 protein-coding genes, 30 tRNA genes, and four rRNA genes. The phylogenetic tree exhibited that I. simonsii and Illicium burmanicum form a sister group, and were nested in the monophyletic clade of the Illicium genus., Competing Interests: No potential conflict of interest was reported by the author(s)., (© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.)

Intracerebral hemorrhage (ICH) is a leading cause of death and disability worldwide. This study aimed to examine the involvement of long non-coding RNAs (lncRNAs), a group of non-coding transcripts, in ICH as potential biomarkers. An expression profile of patients with ICH using four contralateral grey matter controls (GM) and four contralateral white matter controls (WM) was downloaded from the Gene Expression Omnibus (GEO) database. Co-expressed lncRNAs and mRNAs were selected to create competing endogenous RNA (ceRNA) networks. Key lncRNAs were identified in ceRNA networks, which were validated through Real-time qPCR (RT-qPCR) with peripheral blood samples from patients with ICH. A total of 49 differentially expressed lncRNAs were discovered in different brain regions. The ceRNA network in GM included 9 lncRNAs, 40 mRNAs, and 20 microRNAs (miRNAs), while the one in WM covered 6 lncRNAs, 25 mRNAs, and 14 miRNAs. Six hub lncRNAs were observed and RT-qPCR results showed that LY86-AS1 , DLX6-AS1 , RRN3P2 , and CRNDE were down-regulated, while HCP5 and MIAT were up-regulated in patients with ICH. Receiver Operating Characteristic (ROC) assessments demonstrated the diagnostic value of these lncRNAs. Our findings highlight the potential roles of lncRNA in ICH pathogenesis. Moreover, the hub lncRNAs discovered here might become novel biomarkers and promising targets for ICH drug development.

Background: Previous study suggests changes in circRNAs in tumor tissues from cervical squamous cell carcinoma (CSCC) patients. However, little is known about the diagnostic value of circRNAs in CSCC. To assess the potential application of circRNAs as diagnostic tools in CSCC, the circulating circRNAs in peripheral whole blood were carried out., Methods: Five up-regulated circRNAs in peripheral whole blood from 87 patients with CSCC and 55 healthy controls were first identified by real-time quantitative polymerase chain reaction (RT-qPCR). The diagnostic value was evaluated using receiver operating characteristics (ROC) curves and the area under the ROC curves (AUC)., Results: Compared with healthy controls, hsa&#95;circ&#95;0101996, hsa&#95;circ&#95;0104649, hsa&#95;circ&#95;0104443 and hsa&#95;circ&#95;0101119 expression were significantly up-regulated in peripheral whole blood from CSCC patients. ROC analysis showed that hsa&#95;circ&#95;0101996 and hsa&#95;circ&#95;0101119 could distinguish CSCC patients from healthy controls with high AUC (0.906 and 0.887, respectively). Intriguingly, the combination of hsa&#95;circ&#95;0101996 and hsa&#95;circ&#95;0101119 markedly improved AUC (0.964)., Conclusion: All of the findings suggest that hsa&#95;circ&#95;0101996 combined with hsa&#95;circ&#95;0101119 can serve as potential biomarkers for CSCC detection., Competing Interests: None., (IJCEP Copyright © 2017.)

In terms of water quality monitoring based on the ultraviolet-visible spectroscopy, different optical path lengths of spectrometer probe need to be set to maintain higher signal-to-noise ratio of the spectra when the water body measured is complex and changeable. However, large numbers of experiments always have to be undertaken to get the appropriate optical path length, which is difficult to meet the demand of real-time, accurate, sensitive and stable online monitoring system. In this paper, an optimized spectra fusion algorithm was developed to improve the signal-to-noise ratio of fused spectra from two independent spectra that were acquired using two different optical path lengths. In the fusion algorithm, the sliding-pane method was applied to obtain the distribution of noise variance of the spectra, so the region of strong noise in the spectra could be determined. Due to different signal intensity of spectra with long and short optical path length, genetic algorithm was used to calculate the optimal gain matching rate of fusion. Finally, according to the distribution of noise variance, piecewise weighted method is applied to achieve a fusion spectrum with higher signal-to-noise ratio. The experimental results showed that the fusion algorithm could effectively enhance the signal-to-noise ratio of the fused spectra for each sample without altering the optical path length; the noise within 200~250 nm was suppressed and the low-noise and high-sensitivity spectra in the visible band were preserved; Zero interference was moved to the left of 220 nm of the spectrum. This means the fusion algorithm not only shows improvements in both signal-to-noise ratio and the detailed characteristics of the spectrum, but also reduces the excessive number of experiments in order to optimize optical path length and minimize noise in spectra. It has important practical significance to broaden the application range of the ultraviolet-visible spectroscopy based water quality monitoring system.