9 results on '"Yibing Jia"'
Search Results
2. Shu-Xie decoction alleviates oxidative stress and colon injury in acute sleep-deprived mice by suppressing p62/KEAP1/NRF2/HO1/NQO1 signaling
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Mengyuan Wang, Bo Li, Yijiang Liu, Mengting Zhang, Caoxin Huang, Teng Cai, Yibing Jia, Xiaoqing Huang, Hongfei Ke, Suhuan Liu, and Shuyu Yang
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sleep deprivation ,oxidative stress ,NRF2 ,traditional Chinese medicine ,ROS ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Introduction: Sleep disorders are common clinical psychosomatic disorders that can co-exist with a variety of conditions. In humans and animal models, sleep deprivation (SD) is closely related with gastrointestinal diseases. Shu-Xie Decoction (SX) is a traditional Chinese medicine (TCM) with anti-nociceptive, anti-inflammatory, and antidepressant properties. SX is effective in the clinic for treating patients with abnormal sleep and/or gastrointestinal disorders, but the underlying mechanisms are not known. This study investigated the mechanisms by which SX alleviates SD-induced colon injury in vivo.Methods: C57BL/6 mice were placed on an automated sleep deprivation system for 72 h to generate an acute sleep deprivation (ASD) model, and low-dose SX (SXL), high-dose SX (SXH), or S-zopiclone (S-z) as a positive control using the oral gavage were given during the whole ASD-induced period for one time each day. The colon length was measured and the colon morphology was visualized using hematoxylin and eosin (H&E) staining. ROS and the redox biomarkers include reduced glutathione (GSH), malondialdehyde (MDA), and superoxide dismutase (SOD) were detected. Quantitative real-time PCR (qRT-PCR), molecular docking, immunofluorescence and western blotting assays were performed to detect the antioxidant signaling pathways.Results: ASD significantly increased FBG levels, decreased colon length, moderately increased the infiltration of inflammatory cells in the colon mucosa, altered the colon mucosal structure, increased the levels of ROS, GSH, MDA, and SOD activity compared with the controls. These adverse effects were significantly alleviated by SX treatment. ASD induced nuclear translocation of NRF2 in the colon mucosal cells and increased the expression levels of p62, NQO1, and HO1 transcripts and proteins, but these effects were reversed by SX treatment.Conclusion: SX decoction ameliorated ASD-induced oxidative stress and colon injury by suppressing the p62/KEAP1/NRF2/HO1/NQO1 signaling pathway. In conclusion, combined clinical experience, SX may be a promising drug for sleep disorder combined with colitis.
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- 2023
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3. One-Pot Synthesis of C@BiOBr for Efficient Photocatalytic Degradation of Phenol.
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Zhenyu Han, Ya-Ge Liu, Ruixue Zhang, Jiale Shi, Yibing Jia, Xiaochun Liu, and Hai-Ying Jiang
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- 2024
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4. Leveraging human genomic information to identify nonhuman primate sequences for expression array development
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Sergio R. Ojeda, Robert B. Norgren, Yibing Jia, Courtney Gravett, Nicholas F Boyle, Mark A. Pauley, Shaun L. Thompson, and Eliot R. Spindel
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Primates ,DNA, Complementary ,lcsh:QH426-470 ,lcsh:Biotechnology ,Oligonucleotides ,Gene Expression ,Genomics ,Polymerase Chain Reaction ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,law ,lcsh:TP248.13-248.65 ,Chlorocebus aethiops ,Genetics ,Animals ,Humans ,RNA, Messenger ,Cloning, Molecular ,Gene ,Polymerase chain reaction ,DNA Primers ,Oligonucleotide Array Sequence Analysis ,030304 developmental biology ,0303 health sciences ,Models, Genetic ,biology ,Genome, Human ,Gene Expression Profiling ,Exons ,Sequence Analysis, DNA ,biology.organism_classification ,Macaca mulatta ,Gene expression profiling ,Rhesus macaque ,genomic DNA ,lcsh:Genetics ,Genetic Techniques ,Human genome ,DNA microarray ,Algorithms ,030217 neurology & neurosurgery ,Papio ,Research Article ,Biotechnology - Abstract
Background Nonhuman primates (NHPs) are essential for biomedical research due to their similarities to humans. The utility of NHPs will be greatly increased by the application of genomics-based approaches such as gene expression profiling. Sequence information from the 3' end of genes is the key resource needed to create oligonucleotide expression arrays. Results We have developed the algorithms and procedures necessary to quickly acquire sequence information from the 3' end of nonhuman primate orthologs of human genes. To accomplish this, we identified terminal exons of over 15,000 human genes by aligning mRNA sequences with genomic sequence. We found the mean length of complete last exons to be approximately 1,400 bp, significantly longer than previous estimates. We designed primers to amplify genomic DNA, which included at least 300 bp of the terminal exon. We cloned and sequenced the PCR products representing over 5,500 Macaca mulatta (rhesus monkey) orthologs of human genes. This sequence information has been used to select probes for rhesus gene expression profiling. We have also tested 10 sets of primers with genomic DNA from Macaca fascicularis (Cynomolgus monkey), Papio hamadryas (Baboon), and Chlorocebus aethiops (African green monkey, vervet). The results indicate that the primers developed for this study will be useful for acquiring sequence from the 3' end of genes for other nonhuman primate species. Conclusion This study demonstrates that human genomic DNA sequence can be leveraged to obtain sequence from the 3' end of NHP orthologs and that this sequence can then be used to generate NHP oligonucleotide microarrays. Affymetrix and Agilent used sequences obtained with this approach in the design of their rhesus macaque oligonucleotide microarrays.
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- 2005
5. Brain delivery of quercetin-loaded exosomes improved cognitive function in AD mice by inhibiting phosphorylated tau-mediated neurofibrillary tangles
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Yao Qi, Lin Guo, Yibing Jiang, Yijie Shi, Haijuan Sui, and Liang Zhao
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quercetin ,alzheimer’s disease ,exosomes ,bioavailability ,tau ,Therapeutics. Pharmacology ,RM1-950 - Abstract
It is reported that quercetin (Que) can prevent tau pathology and induce neuroprotection by improving cognitive and functional symptoms in the treatment of Alzheimer’s disease (AD). However, its clinical application has been limited due to its poor brain targeting and bioavailability. Exosomes are considered as cargo carriers for intercellular communication and especially serve as a natural and important drug brain delivery platform for achieving better treatment of central neurological diseases. Here, we developed plasma exosomes (Exo) loaded with Que (Exo-Que) to improve the drug bioavailability, enhance the brain targeting of Que and potently ameliorate cognitive dysfunction in okadaic acid (OA)-induced AD mice. Our results showed that Exo-Que improved brain targeting of Que as well as significantly enhanced bioavailability of Que. Furthermore, compared with free Que, Exo-Que better relieved the symptoms of AD by inhibiting cyclin-dependent kinase 5 (CDK5)-mediated phosphorylation of Tau and reducing formation of insoluble neurofibrillary tangles (NFTs), suggesting its therapeutic potential for better treatment of AD.
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- 2020
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6. Chitosan nanoparticles induced the antitumor effect in hepatocellular carcinoma cells by regulating ROS-mediated mitochondrial damage and endoplasmic reticulum stress
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Yibing Jiang, Xiwei Yu, Chang Su, Liang Zhao, and Yijie Shi
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Chitosan ,nanoparticles ,reactive oxygen species ,mitochondrial ,endoplasmic reticulum ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
In recent years, numerous studies have confirmed the role of chitosan nanoparticles (CS NPs) as a promising drug delivery carrier for improving the efficiency of anticancer drug in the treatment of cancer. However, the possible biological effects of CS NPs on tumour cells and underlying mechanisms are still unclear. Recently, reactive oxygen species (ROS)-mediated cell apoptosis has been implicated in the regulation of cell death. In this study, we found that CS NPs induced the massive generation of ROS and resulted in apoptosis of hepatocellular carcinoma cells (SMMC-7721) through activating the mitochondrial pathway and endoplasmic reticulum stress. These results suggest an important role of ROS in CS NPs-induced cancer cell death.
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- 2019
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7. Single nucleotide polymorphisms (SNPs) distinguish Indian-origin and Chinese-origin rhesus macaques (Macaca mulatta).
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Ferguson, Betsy, Street, Summer L, Wright, Hollis, Pearson, Carlo, Yibing Jia, Thompson, Shaun L, Allibone, Patrick, Dubay, Christopher J, Spindel, Eliot, and Norgren Jr, Robert B
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RHESUS monkeys ,GENETIC polymorphisms ,GENES ,NUCLEOTIDES ,MEDICAL genetics - Abstract
Background: Rhesus macaques serve a critical role in the study of human biomedical research. While both Indian and Chinese rhesus macaques are commonly used, genetic differences between these two subspecies affect aspects of their behavior and physiology, including response to simian immunodeficiency virus (SIV) infection. Single nucleotide polymorphisms (SNPs) can play an important role in both establishing ancestry and in identifying genes involved in complex diseases. We sequenced the 3′ end of rhesus macaque genes in an effort to identify gene-based SNPs that could distinguish between Indian and Chinese rhesus macaques and aid in association analysis. Results: We surveyed the 3′ end of 94 genes in 20 rhesus macaque animals. The study included 10 animals each of Indian and Chinese ancestry. We identified a total of 661 SNPs, 457 of which appeared exclusively in one or the other population. Seventy-nine additional animals were genotyped at 44 of the population-exclusive SNPs. Of those, 38 SNPs were confirmed as being population-specific. Conclusion: This study demonstrates that the 3′ end of genes is rich in sequence polymorphisms and is suitable for the efficient discovery of gene-linked SNPs. In addition, the results show that the genomic sequences of Indian and Chinese rhesus macaque are remarkably divergent, and include numerous population-specific SNPs. These ancestral SNPs could be used for the rapid scanning of rhesus macaques, both to establish animal ancestry and to identify gene alleles that may contribute to the phenotypic differences observed in these populations. [ABSTRACT FROM AUTHOR]
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- 2007
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8. X-chromosome inactivation in monkey embryos and pluripotent stem cells
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Hathaitip Sritanaudomchai, Joy Woodward, Cathy Ramsey, Yibing Jia, Masahito Tachibana, Hong Ma, Keith Masterson, Shoukhrat Mitalipov, Erin E. Wolff, Michelle Sparman, and Hyo Sang Lee
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Male ,Pluripotent Stem Cells ,Primates ,Embryonic stem cells ,X Chromosome ,Biology ,Article ,X-inactivation ,Genomic Imprinting ,03 medical and health sciences ,0302 clinical medicine ,Genes, X-Linked ,X Chromosome Inactivation ,Animals ,Inner cell mass ,Cell Lineage ,Epigenetics ,Induced pluripotent stem cell ,Molecular Biology ,X chromosome ,reproductive and urinary physiology ,030304 developmental biology ,0303 health sciences ,Cell Biology ,Embryo, Mammalian ,Macaca mulatta ,Embryonic stem cell ,Molecular biology ,Blastocyst ,embryonic structures ,Female ,XIST ,Genomic imprinting ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Inactivation of one X chromosome in female mammals (XX) compensates for the reduced dosage of X-linked gene expression in males (XY). However, the inner cell mass (ICM) of mouse preimplantation blastocysts and their in vitro counterparts, pluripotent embryonic stem cells (ESCs), initially maintain two active X chromosomes (XaXa). Random X chromosome inactivation (XCI) takes place in the ICM lineage after implantation or upon differentiation of ESCs, resulting in mosaic tissues composed of two cell types carrying either maternal or paternal active X chromosomes. While the status of XCI in human embryos and ICMs remains unknown, majority of human female ESCs show non-random XCI. We demonstrate here that rhesus monkey ESCs also display monoallelic expression and methylation of X-linked genes in agreement with non-random XCI. However, XIST and other X-linked genes were expressed from both chromosomes in isolated female monkey ICMs indicating that ex vivo pluripotent cells retain XaXa. Intriguingly, the trophectoderm (TE) in preimplantation monkey blastocysts also expressed X-linked genes from both alleles suggesting that, unlike the mouse, primate TE lineage does not support imprinted paternal XCI. Our results provide insights into the species-specific nature of XCI in the primate system and reveal fundamental epigenetic differences between in vitro and ex vivo primate pluripotent cells.
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9. Temporal Variation and Chemical Components of Rural Ambient PM2.5 during Main Agricultural Activity Periods in the Black Soil Region of Northeast China
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Xuewei Wu, Weiwei Chen, Shichun Zhang, Ruimin Li, Mengduo Zhang, Juan Liu, Yibing Jiang, and Yang Liu
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PM2.5 concentration ,rural ,water-soluble ions ,trace elements ,Northeast China ,Meteorology. Climatology ,QC851-999 - Abstract
Agricultural emissions are crucial to regional air quality in the autumn and spring due to the intense agricultural activities in Northeast China. However, information on rural ambient particulate matter (PM) in Northeast China is rare, limiting the accurate estimation of agricultural atmospheric particulate matter emissions. In this study, we monitored hourly ambient PM2.5 (PM with a diameter of less than 2.5 μm) concentrations and analyzed daily chemical components (i.e., water-soluble ions, trace elements, organic carbon, and element carbon) at a rural site in Northeast China during the autumn and spring and assessed the impact of agricultural activities on atmospheric PM2.5 concentrations. The results showed that the daily average concentrations of PM2.5 were 143 ± 109 (range: 39−539) μg m−3 from 19 October to 23 November 2017 (i.e., typical harvesting month) and 241 ± 189 (range: 97−976) μg m−3 from 1 April to 13 May 2018 (i.e., typical tilling month). In autumn, the ambient PM2.5 concentrations were high with a Southwest wind, while a Southeast wind caused high PM2.5 concentrations during spring in the rural site. The concentrations of selected water-soluble ions, trace elements, and carbonaceous fractions accounted for 33%, 4%, and 26% of PM2.5 mass concentrations, respectively, in autumn and for 10%, 5%, and 3% of PM2.5 mass concentrations, respectively, in spring. On the basis of the component analysis, straw burning, agricultural machinery, and soil dust driven by wind and tilling were the main contributors to high rural PM2.5 concentrations. In addition, the increasing coal combustion around the rural site was another important source of PM2.5.
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- 2019
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