31 results on '"Yoshihiro Okamoto"'
Search Results
2. Diploid Male Gametes Circumvent Hybrid Sterility Between Asian and African Rice Species
- Author
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Daichi Kuniyoshi, Itaru Masuda, Yoshitaka Kanaoka, Yuki Shimazaki-Kishi, Yoshihiro Okamoto, Hideshi Yasui, Toshio Yamamoto, Kiyotaka Nagaki, Yoichiro Hoshino, Yohei Koide, Itsuro Takamure, and Yuji Kishima
- Subjects
anther culture ,division restitution ,hybrid sterility ,interspecific hybrid ,meiosis ,rice ,Plant culture ,SB1-1110 - Abstract
In F1 hybrids of Oryza sativa (Asian rice) and Oryza glaberrima (African rice), heterozygosity leads to a complete gamete abortion because of allelic conflict at each of the 13 hybrid sterility (HS) loci. We systematically produced 19 plants from the F1 hybrids of both the rice species by the anther culture (AC) method. Five of the 19 interspecific hybrid plants were partially fertile and able to produce seeds. Unlike ordinal doubled haploid plants resulting from AC, these regenerated plants showed various ploidy levels (diploid to pentaploid) and different zygosities (completely homozygous, completely heterozygous, and a combination). These properties were attributable to meiotic anomalies in the interspecific hybrid F1 plants. Examination of the genetic structures of the regenerated plants suggested meiotic non-reduction took place in the interspecific hybrid F1 plants. The centromeric regions in the regenerated plants revealed that the abnormal first and/or second divisions of meiosis, namely the first division restitution (FDR) and/or second division restitution (SDR), had occurred in the interspecific hybrid. Immunohistochemical observations also verified these phenomena. FDR and SDR occurrences at meiosis might strongly lead to the formation of diploid microspores. The results demonstrated that meiotic anomalies functioned as a reproductive barrier occurred before the HS genes acted in gamete of the interspecific hybrid. Although such meiotic anomalies are detrimental to pollen development, the early rescue of microspores carrying the diploid gamete resulted in the fertile regenerated plants. The five partially fertile plants carrying tetraploid genomes with heterozygous alleles of the HS loci produced fertile diploid pollens, implying that the diploid gametes circumvented the allelic conflicts at the HS loci. We also proposed how diploid male gametes avoid HS with the killer–protector model.
- Published
- 2020
- Full Text
- View/download PDF
3. Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
- Author
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Yoshitaka Kanaoka, Daichi Kuniyoshi, Eri Inada, Yohei Koide, Yoshihiro Okamoto, Hideshi Yasui, and Yuji Kishima
- Subjects
Anther culture ,Callus ,Hybrid sterility ,Mapping ,Microspore ,Oryza sativa ,Plant culture ,SB1-1110 ,Biology (General) ,QH301-705.5 - Abstract
Abstract Background To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood. Results Microspores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S 1 and S 19 loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD. Conclusions We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes.
- Published
- 2018
- Full Text
- View/download PDF
4. Morphological Characterization of Tetraploids of Limonium sinuatum (L.) Mill. Produced by Oryzalin Treatment of Seeds
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Shiro Mori, Masaki Yahata, Ayano Kuwahara, Yurina Shirono, Yasufumi Ueno, Misaki Hatanaka, Yoshimi Honda, Keita Sugiyama, Naho Murata, Yoshihiro Okamoto, and Takahiro Wagatsuma
- Subjects
bolting ,cut flower ,germinal pore ,ornamental plant ,polyploidy periclinal chimera ,Plumbaginaceae ,Plant culture ,SB1-1110 - Abstract
Limonium sinuatum (L.) Mill. (2n = 2x = 16) is a popular ornamental plant with dimorphism of pollen grains (type A and type B) and stigmas (papilla and cob-like). We applied polyploidy breeding to this species in order to introduce desirable traits. Tetraploid and mixoploid L. sinuatum plants were successfully obtained with oryzalin treatment of L. sinuatum ‘Early Blue’ seeds. All three tetraploids had increased leaf width, stomatal size, flower length, and pollen width compared to those of the diploid, and tetraploids had four germinal pores of pollen grains, whereas the diploid had three. All tetraploids had type A pollen grains and cob-like stigmas. Furthermore, the growth of cultivated tetraploid plants was slow, with later bolting and flowering times. Mixoploids Mixo-1 and Mixo-3 were estimated to be polyploidy periclinal chimeric plants consisting of a tetraploid L1 layer and diploid L2 layer, and Mixo-2 was estimated to be a polyploidy periclinal chimeric plant consisting of the diploid L1 layer and tetraploid L2 layer. Mixo-4 had tetraploid L1 and L2 layers. Mixoploids, except Mixo-4, had type A pollen grains and cob-like stigmas, whereas Mixo-4 had type B pollen grains and papilla stigmas. These polyploids will be useful as polyploidy breeding materials.
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- 2021
- Full Text
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5. Mechanism of Cs Removal from Fukushima Weathered Biotite by Heat Treatment with a NaCl–CaCl2 Mixed Salt
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Mitsunori Honda, Yoshihiro Okamoto, Iwao Shimoyama, Hideaki Shiwaku, Shinichi Suzuki, and Tsuyoshi Yaita
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Chemistry ,QD1-999 - Published
- 2017
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6. Japanese Social Exclusion and Inclusion from a Housing Perspective
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Yoshihiro Okamoto
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corporate welfare ,economic poverty ,housing perspective ,informal mutual aid ,Non-profit Organisations ,Sociology (General) ,HM401-1281 - Abstract
This paper examines conditions of social exclusion and attempts at social inclusion in Japan from a housing perspective. Companies, households and the government have previously supported housing in Japan. However, corporate welfare was withdrawn following the globalization of the economy from the 1990s onwards, support from families and communities declined due to a reduction in household size, and governmental housing support has shifted away from direct support. A reduction in income and unstable work left many people with unstable housing. Certain workers, such as foreigners performing dispatched labour, could not maintain continuous work under the influence of the Lehman Brothers’ bankruptcy in 2008. Household size has shrunk according to changes in the industrial structure, and the number of households that cannot sustain housing is increasing. Such vulnerable households—elderly people, the handicapped, low-income earners and single parents—can become excluded from the rental housing market. On the other hand, governmental measures are promoting local dwellings and maintaining the condition for a dwelling service. Activities, such as local community support of the homeless have been initiated by various Non-profit Organisations (NPOs) and NPO activities are increasingly exemplifying measures to achieve social inclusion.
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- 2016
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7. Leptospirosis in Squirrels Imported from United States to Japan
- Author
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Toshiyuki Masuzawa, Yoshihiro Okamoto, Yumi Une, Takahiro Takeuchi, Keiko Tsukagoshi, Nobuo Koizumi, Hiroki Kawabata, Shuji Ohta, and Yasuhiro Yoshikawa
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Exotic animals ,Leptospira ,Leptospirosis ,Southern flying squirrel ,Japan ,United States ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We diagnosed leptospirosis in 2 patients exposed to southern flying squirrels imported from the United States to Japan. Patients worked with exotic animals in their company. Leptospira isolates from 1 patient and 5 of 10 squirrels at the company were genetically and serologically identical and were identified as Leptospira kirschneri.
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- 2006
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- View/download PDF
8. Anther culture in rice proportionally rescues microspores according to gametophytic gene effect and enhances genetic study of hybrid sterility
- Author
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Yoshihiro Okamoto, Hideshi Yasui, Yoshitaka Kanaoka, Daichi Kuniyoshi, Eri Inada, Yuji Kishima, and Yohei Koide
- Subjects
0106 biological sciences ,0301 basic medicine ,Anther culture ,Sterility ,Population ,Stamen ,Oryza sativa ,Plant Science ,Biology ,lcsh:Plant culture ,01 natural sciences ,Callus ,03 medical and health sciences ,Microspore ,Genetics ,lcsh:SB1-1110 ,education ,lcsh:QH301-705.5 ,Hybrid ,education.field_of_study ,Research ,Chromosome ,food and beverages ,O. glaberrima ,030104 developmental biology ,Hybrid sterility ,Mapping ,lcsh:Biology (General) ,Rice ,010606 plant biology & botany ,Biotechnology - Abstract
Background To investigate plant hybrid sterility, we studied interspecific hybrids of two cultivated rice species, Asian rice (Oryza sativa) and African rice (O. glaberrima). Male gametes of these hybrids display complete sterility owing to a dozen of hybrid sterility loci, termed HS loci, but this complicated genetic system remains poorly understood. Results Microspores from these interspecific hybrids form sterile pollen but are viable at the immature stage. Application of the anther culture (AC) method caused these immature microspores to induce callus. The segregation distortion of 11 among 13 known HS loci was assessed in the callus population. Using many individual calli, fine mapping of the HS loci was attempted based on heterozygotes produced from chromosome segment substitution lines (CSSLs). Transmission ratio distortion (TRD) from microspores was detected at 6 of 11 HS loci in the callus population. The fine mapping of S1 and S19 loci using CSSLs revealed precise distances of markers from the positions of HS loci exhibiting excessive TRD. Conclusions We demonstrated that AC to generate callus populations derived from immature microspores is a useful methodology for genetic study. The callus population facilitated detection of TRD at multiple HS loci and dramatically shortened the process for mapping hybrid sterility genes. Electronic supplementary material The online version of this article (10.1186/s13007-018-0370-z) contains supplementary material, which is available to authorized users.
- Published
- 2018
9. Mechanism of Cs Removal from Fukushima Weathered Biotite by Heat Treatment with a NaCl–CaCl2 Mixed Salt
- Author
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Iwao Shimoyama, Mitsunori Honda, Shinichi Suzuki, Yoshihiro Okamoto, Tsuyoshi Yaita, and Hideaki Shiwaku
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In situ ,chemistry.chemical_classification ,010504 meteorology & atmospheric sciences ,Extended X-ray absorption fine structure ,Chemistry ,General Chemical Engineering ,Structure function ,Salt (chemistry) ,General Chemistry ,010501 environmental sciences ,engineering.material ,01 natural sciences ,Fluorescence ,Article ,lcsh:Chemistry ,lcsh:QD1-999 ,engineering ,Absorption (chemistry) ,Biotite ,0105 earth and related environmental sciences ,Nuclear chemistry - Abstract
An in situ extended X-ray absorption fine structure (in situ EXAFS) spectroscopic analysis at high temperature was conducted to investigate the mechanism of Cs removal from weathered biotite (WB) from Fukushima, induced by heating with a mixed salt of NaCl and CaCl2. This indicated that most Cs remained in WB during heating at 200–700 °C. In addition, the in situ EXAFS spectra gradually changed on heating with the mixed salt and a completely different spectrum was observed for the sample after cooling from 700 °C to room temperature (RT). Ex situ EXAFS measurements and X-ray fluorescence analyses were also conducted on samples after heat treatment and removal of the mixed salt to clarify the temperature dependence of the Cs removal ratio. On the basis of the results of radial structure function analysis obtained from in situ EXAFS, we concluded that almost all of the Cs was removed from WB by heating at 700 °C with the mixed salt, and that Cs formed Cs–Cl bonds after cooling to RT from 700 °C. In contrast, although more than half of the Cs present was removed from WB by heat treatment at 500 °C, most Cs was surrounded by silica tetrahedrons, maintained by Cs–O bonds. On the basis of these results, different Cs removal processes are suggested for the high-temperature (600–700 °C) and low-temperature (400–500 °C) regions.
- Published
- 2017
10. Diploid Male Gametes Circumvent Hybrid Sterility Between Asian and African Rice Species
- Author
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Yuki Shimazaki-Kishi, Yohei Koide, Itaru Masuda, Yuji Kishima, Kiyotaka Nagaki, Yoichiro Hoshino, Yoshitaka Kanaoka, Daichi Kuniyoshi, Toshio Yamamoto, Yoshihiro Okamoto, Hideshi Yasui, and Itsuro Takamure
- Subjects
0106 biological sciences ,0301 basic medicine ,Sterility ,interspecific hybrid ,Plant Science ,Biology ,lcsh:Plant culture ,medicine.disease_cause ,01 natural sciences ,hybrid sterility ,03 medical and health sciences ,Microspore ,Meiosis ,Pollen ,medicine ,meiosis ,lcsh:SB1-1110 ,diploid gamete ,Original Research ,Hybrid ,Genetics ,anther culture ,rice ,fungi ,food and beverages ,division restitution ,tetraploid ,030104 developmental biology ,medicine.anatomical_structure ,Doubled haploidy ,Gamete ,Ploidy ,010606 plant biology & botany - Abstract
In F1hybrids ofOryza sativa(Asian rice) andO. glaberrima(African rice), heterozygosity leads to a complete gamete abortion because of allelic conflict at each of the 13hybrid sterility(HS) loci. We systematically produced 19 plants from the F1hybrids of both the rice species by the anther culture (AC) method. Five of the 19 interspecific hybrid plants were fertile and able to produce seeds. Unlike ordinal doubled haploid plants resulting from AC, these regenerated plants showed various ploidy levels (diploid to pentaploid) and different zygosities (completely homozygous, completely heterozygous, and a combination). These properties were attributable to meiotic anomalies in the interspecific hybrid F1plants. Examination of the genetic structures of the regenerated plants suggested meiotic non-reduction took place in the interspecific hybrid F1plants. The centromeric regions in the regenerated plants revealed that the abnormal first and/or second divisions of meiosis, namely the first division restitution (FDR) and/or second division restitution (SDR), had occurred in the interspecific hybrid. Immunohistochemical observations also verified these phenomena. FDR and SDR occurrences at meiosis might strongly lead to the formation of diploid microspores. The results demonstrated that meiotic anomalies functioned as a reproductive barrier occurred before theHSgenes acted in gamete of the interspecific hybrid. Although such meiotic anomalies are detrimental to pollen development, the early rescue of microspores carrying the diploid gamete resulted in the fertile regenerated plants. The five fertile plants carrying tetraploid genomes with heterozygous alleles of theHSloci produced fertile diploid pollens, implying that the diploid gametes circumvented the allelic conflicts at theHSloci. We also proposed how diploid male gametes avoid HS with the killer-protector model.
- Published
- 2020
11. Implementation of computer simulation to assess flow diversion treatment outcomes: systematic review and meta- analysis.
- Author
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Mingzi Zhang, Simon Tupin, Hitomi Anzai, Yutaro Kohata, Masaaki Shojima, Kosuke Suzuki, Yoshihiro Okamoto, Katsuhiro Tanaka, Takanobu Yagi, Soichiro Fujimura, and Makoto Ohta
- Subjects
BIOMECHANICS ,COMPUTER simulation ,HEMODYNAMICS ,INTRACRANIAL aneurysms ,META-analysis ,SHEAR (Mechanics) ,SURGICAL stents ,SYSTEMATIC reviews ,TREATMENT effectiveness ,HUMAN services programs - Abstract
Introduction Despite a decade of research into virtual stent deployment and the post- stenting aneurysmal hemodynamics, the hemodynamic factors which correlate with successful treatment remain inconclusive. We aimed to examine the differences in various post- treatment hemodynamic parameters between successfully and unsuccessfully treated cases, and to quantify the additional flow diversion achievable through stent compaction or insertion of a second stent. Methods A systematic review and meta- analysis were performed on eligible studies published from 2000 to 2019. We first classified cases according to treatment success (aneurysm occlusion) and then calculated the pooled standardized mean differences (SMD) of each available parameter to examine their association with clinical outcomes. Any additional flow diversion arising from the two common strategies for improving the stent wire density was quantified by pooling the results of such studies. Results We found that differences in the aneurysmal inflow rate (SMD -6.05, 95% CI -10.87 to -1.23, p=0.01) and energy loss (SMD -5.28, 95% CI -7.09 to -3.46, p<0.001) between the successfully and unsuccessfully treated groups were indicative of statistical significance, in contrast to wall shear stress (p=0.37), intra- aneurysmal average velocity (p=0.09), vortex core- line length (p=0.46), and shear rate (p=0.09). Compacting a single stent could achieve additional flow diversion comparable to that by dualstent implantation. Conclusions Inflow rate and energy loss have shown promise as identifiers to discriminate between successful and unsuccessful treatment, pending future research into their diagnostic performance to establish optimal cut- off values. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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12. Preliminary study of eye tracking to investigate the differences in gaze behaviors depending on the experience of neuroendovascular therapy.
- Author
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Masaaki Shojima, Yoshihiro Okamoto, Kuniyasu Niizuma, Makoto Ohta, Osamu Ishikawa, Ayano Fujisawa, Hiroyuki Tsukihara, Nobuyuki Sakai, and Teiji Tominaga
- Subjects
GAZE ,EYE tracking ,ENDOVASCULAR surgery ,EYE movements ,EXPERIENCE ,THERAPEUTIC embolization - Abstract
Background: Neuroendovascular therapy is now the choice for the management of many neurovascular pathologies, and physicians with endovascular skills are in high demand. In addition to the traditional method of practicing hand movements to learn skills, a new strategy of practicing eye movements to learn skills is also attracting attention. This preliminary study explored the differences in gaze behavior depending on experience with endovascular procedures to be facilitated in future skill training in neuroendovascular therapy. Methods: Four physicians with experience of 3-412 neuroendovascular procedures wore eye-tracking devices during coil embolization of swine cervical arteries. Gaze metrics with direct correlations to the expertise of endovascular procedures were explored. Results: Gaze metrics with a positive direct correlation to experience included the proportion of fixation durations (PFD) in the screen area and the native images. Those with a negative direct correlation included the PFD in the offscreen area and the roadmap images and the average fixation durations in the off-screen and coil areas. During the parent artery occlusion procedure with detachable coils, more experienced operators preferred to look at native images rather than roadmap images and that less experienced operators tended to look down at their hands more frequently. Conclusion: This preliminary study demonstrated the feasibility of eye tracking to identify the differences in gaze behavior depending on the experience of endovascular procedures and may guide future eye-tracking studies in neuroendovascular therapy. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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13. Local coordination about La(3+) in molten LaCl3 and its mixtures with alkali chlorides
- Author
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Yoshihiro Okamoto, Shinichi Suzuki, Paul A. Madden, Tsuyoshi Yaita, Hideaki Shiwaku, and Atsushi Ikeda-Ohno
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Alkaline earth metal ,Chemistry ,Inorganic chemistry ,Qualitative inorganic analysis ,Physical and Theoretical Chemistry ,Absorption (chemistry) ,Alkali metal ,Local structure ,Ion ,X-ray absorption fine structure - Abstract
The local structure around the La(3+) ions in molten LaCl(3) and its mixtures with alkali and alkaline earth chlorides has been investigated by using extended X-ray absorption fine structure (XAFS) and molecular dynamics (MD) techniques. Such mixtures, which are of current technological interest, are known to be thermodynamically nonideal, and there has been a good deal of work to understand the structural effects factors that contribute to the nonideality. New experimental methods allow observations at the La K-absorption edge at the high temperatures of interest, and the ability of the technique to obtain reliable information even at very low La(3+) concentrations in multicomponent mixtures is demonstrated. Both the mean La(3+)-Cl(-) interionic separation and the mean La(3+) coordination number are found to decrease as the concentration of La(3+) in the mixture decreases. The rate of decrease depends on the identity of the alkali and alkaline earth cations present in the mixtures in a way that parallels the degree of nonideality of the different systems; it is greatest for those alkali cations that coordinate Cl(-) weakly. In dilute mixtures with such cations La(3+) is able to adopt a very stable octahedral coordination geometry but this is inhibited by the presence of more strongly coordinating cations like Li(+) and Mg(2+).
- Published
- 2016
14. Structural investigation of thorium in molten lithiumecalcium fluoride mixtures for salt treatment process in molten salt reactor
- Author
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Keisuke Tajima, Yoshihiro Okamoto, Yasuaki Shimohara, Hiroshi Akatsuka, Catherine Bessada, Masahiko Numakura, Atsushi Nezu, Hirokazu Kawano, Haruaki Matsuura, Takeshi Nakahagi, and Nobuaki Sato
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chemistry.chemical_classification ,Materials science ,Molten salt reactor ,Inorganic chemistry ,Energy Engineering and Power Technology ,Lithium fluoride ,chemistry.chemical_element ,Salt (chemistry) ,Thorium ,X-ray absorption fine structure ,law.invention ,chemistry.chemical_compound ,Nuclear Energy and Engineering ,chemistry ,law ,Fluorine ,Lithium ,Safety, Risk, Reliability and Quality ,Waste Management and Disposal ,Fluoride - Abstract
X-ray absorption fine structure (XAFS) measurements on thorium fluoride in molten lithium–calcium fluoride mixtures and molecular dynamics (MD) simulation of zirconium and yttrium fluoride in molten lithium–calcium fluoride mixtures have been carried out. In the molten state, coordination number of thorium (Ni) and inter ionic distances between thorium and fluorine in the first neighbor (ri) are nearly constant in all mixtures. However the fluctuation factors (Debye–Waller factor (σ2) and C3 cumulant) increase until xCaF2 = 0.17 and decrease by addition of excess CaF2. It means that the local structure around Th4+ is disordered until xCaF2 = 0.17 and stabilized over xCaF2 = 0.17. The variation of fluctuation factors is related to the number density of F− in ThF4 mixtures and the stability of local structure around Th4+ increases with decreasing the number density of F− in ThF4 mixtures. This tendency is common to those in the ZrF4 and YF3 mixtures. However, in the case of YF3 mixtures, the local structure around Y3+ becomes disordered until xCaF2 = 0.40 and it becomes stabilized by addition of excess CaF2. The difference between ThF4 mixtures and YF3 mixtures is related to the difference of Coulumbic interaction between Th4+–F− and Y3+–F−. Therefore, the variation of local structure around cation is related to not only number density of F− in molten salts but also the Coulumbic interaction between cation and anion.
- Published
- 2011
15. Temperature dependence of short-range structural property in lithium - lead fluoride
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Yoshihiro Okamoto, Takeshi Sakamoto, Reo Toyoyoshi, Sou Watanabe, Yasuhiko Iwadate, Hiroshi Akatsuka, and Haruaki Matsuura
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Chemistry ,Coordination number ,Analytical chemistry ,chemistry.chemical_element ,General Chemistry ,Atmospheric temperature range ,Condensed Matter Physics ,X-ray absorption fine structure ,Ion ,Absorption edge ,Melting point ,General Materials Science ,Lithium ,Absorption (chemistry) ,Nuclear chemistry - Abstract
In this work, X-ray Absorption Fine Structure (XAFS) measurements of PbF 2 and LiF–PbF 2 (PbF 2 : 60% mol) were carried out with Pb–L III X-ray Absorption edge to investigate local structure of molten LiF–PbF 2 which we have proposed as one of the candidates for fusion reactor blanket. We performed the measurements with wide temperature range from room temperature to above the melting point. We obtained structural information around Pb 2+ ion depending on the temperature. The nearest Pb 2+ –F − distance and coordination number were estimated to be 2.36 A and 6, respectively, for molten LiF–PbF 2 , while they are 2.27 A and 4 for molten PbF 2 .
- Published
- 2005
16. Influence of writing and reading intertrack interferences in terms of bit aspect ratio in shingled magnetic recording.
- Author
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Hirofumi Nobuhara, Yoshihiro Okamoto, Masato Yamashita, Yasuaki Nakamura, Hisashi Osawa, and Hiroaki Muraoka
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SIMULATION methods & models , *MAGNETIC recording media , *ASTEROIDS , *RANDOM noise theory , *VORONOI polygons - Abstract
In this paper, we investigate the influence of the writing and reading intertrack interferences (ITIs) in terms of bit aspect ratio (BAR) in shingled magnetic recording by computer simulation using a read/write model which consists of a writing process based on Stoner-Wohlfarth switching asteroid by a one-side shielded isosceles triangular write head and a reading process by an around shielded read head for a discrete Voronoi medium model. The results show that BAR should be 3 to reduce the influence of writing and reading ITIs, media noise, and additive white Gaussian noise in an assumed areal density of 4.61Tbpsi. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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17. Age-Related Changes in Soluble Vascular Endothelial Growth Factor Receptor 1 (sVEGFR1) and Receptor 2 (sVEGFR2) in Healthy Japanese Subjects
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Yoshihiro Okamoto, Testuri Kodama, Tomoyo Mita, Naoya Fujita, Toshiyuki Masuzawa, Takashi Fukui, and Yoshimitsu Gotoh
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medicine.medical_specialty ,Short Communication ,Clinical Biochemistry ,Biology ,medicine.disease ,Diarrhea ,Future study ,Endocrinology ,Hemolytic uremic syndrome (HUS) ,Internal medicine ,Age related ,medicine ,medicine.symptom ,Receptor ,Pathological - Abstract
We measured serum soluble vascular endothelial growth factor receptor 1 (sVEGFR1) and receptor 2 (sVEGFR2) levels in healthy Japanese individuals in order to establish a reference value using a specific ELISA. Significant differences were observed in serum sVEGFR1 and sVEGFR2 levels between children and adults. To demonstrate the usefulness of the reference value for children, we measured serum sVEGFR1 and sVEGFR2 levels in children with diarrhea positive (D+) hemolytic uremic syndrome (HUS) as a preliminary study. Serum sVEGFR2 levels in children with HUS were markedly higher than those in healthy children from the onset of D + HUS. The reference value for healthy children in the present study will allow normal and pathological conditions to be discriminated from each other in future study.
- Published
- 2014
18. Local structural analyses on molten terbium fluoride in lithium fluoride and lithium–calcium fluoride mixtures
- Author
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Atsushi Nezu, Masahiko Numakura, Yoshihiro Okamoto, Pierre Chamelot, Hideaki Shiwaku, Olivier Pauvert, Catherine Bessada, Hiroshi Akatsuka, Haruaki Matsuura, Keisuke Tajima, Tsuyoshi Yaita, Yasuaki Shimohara, Didier Zanghi, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Japan Atomic Energy Agency - JAEA (JAPAN), LE STUDIUM (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Tokyo Institute of Technology - Tokyo Tech (JAPAN), and Université d'Orléans (FRANCE)
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Chemistry ,X-ray absorption fine structure (XAFS) ,Organic Chemistry ,Inorganic chemistry ,Lithium fluoride ,chemistry.chemical_element ,Terbium ,Biochemistry ,X-ray absorption fine structure ,Inorganic Chemistry ,chemistry.chemical_compound ,Terbium fluoride ,Fluorine ,Mécanique des solides ,Environmental Chemistry ,Génie chimique ,Molten salt ,Lithium ,Physical and Theoretical Chemistry ,Physique Atomique ,Ternary operation ,Fluoride ,Calcium fluoride - Abstract
X-ray absorption fine structure (XAFS) measurements on terbium fluoride in molten lithium fluoride and in molten lithium–calcium fluoride mixtures, (e.g. 0.20TbF3–0.80LiF, 0.20TbF3–0.62LiF–0.18CaF2, 0.20TbF3–0.48LiF–0.32CaF2, 0.50TbF3–0.50LiF, and 0.50TbF3–0.38LiF–0.12CaF2), have been carried out. In the solid state, coordination number of terbium (Ni) and inter ionic distances between terbium and fluorine in the first neighbor (ri) are nearly constant in all mixtures. In 0.20TbF3–0.80LiF, 0.20TbF3–0.62LiF–0.18CaF2 and 0.50TbF3–0.50LiF mixtures, Ni's decrease from ca. 8 to 6 and ri's also decrease from ca. 2.29 to 2.26 A on melting. On the other hands, in molten 0.20TbF3–0.48LiF–0.32CaF2 and 0.50TbF3–0.38LiF–0.12CaF2 mixtures, Ni's are slightly larger than 6 and ri's do not change. These facts correspond to the amount of F− supplied by solvent melts, i.e. the effect of CaF2 becomes predominant at bCaF2 > 0.32 in ternary 0.20TbF3–aLiF–bCaF2 mixtures and at bCaF2 > 0.12 in ternary 0.50TbF3–aLiF–bCaF2 mixtures.
- Published
- 2010
19. X-ray diffraction and molecular dynamics simulation studies of molten uranium chloride
- Author
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Yoshihiro Okamoto, Kazuo Minato, and Paul A. Madden
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Diffraction ,Nuclear and High Energy Physics ,Chemistry ,Diffusion ,Coordination number ,Mineralogy ,Thermodynamics ,Molecular dynamics ,Viscosity ,Nuclear Energy and Engineering ,Polarizability ,Electrical resistivity and conductivity ,X-ray crystallography ,General Materials Science - Abstract
The structure of molten UCl3 at 1200 K was studied by high-temperature X-ray diffraction and molecular dynamics simulation. The XRD data was reproduced by the simulation with a polarizable ionic model. The nearest U3+-Cl- distance was 2.85 Å with coordination number 8.0, implying that the 8-fold structure (UCl 8)5- is predominant in the melt - in contradiction to earlier suggestions of octahedral coordination. The potential model, which had been optimized by comparison with the structural data, was also found to reproduce the experimental information on transport properties like the diffusion coefficient, electrical conductivity and shear viscosity. © 2005 Elsevier B.V. All rights reserved.
- Published
- 2005
20. Structural study of molten lanthanum halides by X-ray diffraction and computer simulation techniques
- Author
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Yoshihiro Okamoto and P.A. Madden
- Subjects
Diffraction ,Crystallography ,Molecular dynamics ,Ionic model ,Polarizability ,Chemistry ,X-ray crystallography ,Lanthanum ,Halide ,chemistry.chemical_element ,General Materials Science ,General Chemistry ,Condensed Matter Physics - Abstract
The structure of molten LaCl3 was investigated by using a high temperature X-ray diffraction with a molecular dynamics simulation technique. The obtained XRD data was nicely reproduced by the MD simulation with polarizable ionic model. The results show that 7-fold (LaCl7) 4- and/or 8-fold (LaCl8)5- coordination structures are predominant in the melt. It is concluded that the structure of molten LaCl3 is isomorphous with that of molten LaBr3. © 2004 Elsevier Ltd. All rights reserved.
- Published
- 2005
21. Safety operation of chromatography column system with discharging hydrogen radiolytically generated.
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Sou Watanabe, Yuichi Sano, Kazunori Nomura, Yoshikazu Koma, and Yoshihiro Okamoto
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CHROMATOGRAPHIC analysis ,HYDROGEN ,MOBILE phase (Chromatography) ,FLUID dynamics ,GLOW discharges - Abstract
In the extraction chromatography system, accumulation of hydrogen gas in the chromatography column is suspected to lead to fire or explosion. In order to prevent the hazardous accidents, it is necessary to evaluate behaviors of gas radiolytically generated inside the column. In this study, behaviors of gas inside the extraction chromatography column were investigated through experiments and Computation Fluid Dynamics (CFD) simulation. N
2 gas once accumulated as bubbles in the packed bed was hardly discharged by the flow of mobile phase. However, the CFD simulation and X-ray imaging on g-ray irradiated column revealed that during operation the hydrogen gas generated in the column was dissolved into the mobile phase without accumulation and discharged. [ABSTRACT FROM AUTHOR]- Published
- 2015
- Full Text
- View/download PDF
22. XAFS analysis of molten rare earth-alkali metal fluoride systems
- Author
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Sou Watanabe, Akihiko Kajinami, T. Honma, Haruaki Matsuura, Ashok K. Adya, Takeshi Sakamoto, H. Deguchi, M. Horiuchi, T. Yamamoto, Yoshihiro Okamoto, N. Kitamura, Keiichi Takase, Norimasa Umesaki, M. Hatcho, Hiroshi Akatsuka, and Satoru Noguchi
- Subjects
Lanthanide ,Extended X-ray absorption fine structure ,Chemistry ,Mechanical Engineering ,Coordination number ,Metals and Alloys ,Analytical chemistry ,Alkali metal ,X-ray absorption fine structure ,Metal ,chemistry.chemical_compound ,Mechanics of Materials ,visual_art ,Materials Chemistry ,visual_art.visual_art_medium ,Molten salt ,Fluoride ,Nuclear chemistry - Abstract
X-ray absorption fine structure (XAFS) measurements on rare-earth-alkali metal fluoride systems (0.2 LnF 3 –0.8 MF: Ln = La, Ce, Nd, Sm; M = Li, Na, K) have been carried out at 300 and 1173 K. The local structures around rare-earth-ions in both the solid and liquid phases were evaluated by the curve-fitting analysis, involving anharmonic oscillation effects at the higher temperature. This study revealed that the nearest-neighbour Ln 3+ –F − distance and the corresponding coordination number of F − around Ln 3+ in the molten state mainly depend on the lanthanide species rather than the size of the alkali metal ion.
- Published
- 2004
23. Structural properties of the inner coordination sphere of indium chloride complexes in organic and aqueous solutions.
- Author
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Hirokazu Narita, Mikiya Tanaka, Hideaki Shiwaku, Yoshihiro Okamoto, Shinichi Suzuki, Atsushi Ikeda-Ohno, and Tsuyoshi Yaita
- Subjects
INDIUM chlorides ,AQUEOUS solutions ,SOLVENT extraction ,COORDINATE covalent bond ,TERTIARY amines - Abstract
The nature of the inner coordination sphere of In
3+ present in both the organic and aqueous solutions during the solvent extraction of In3+ from an aqueous HCl solution with tri-n-octyl amine (TOA) was investigated by In K-edge XAFS. This information was then used to clarify the details of the extraction properties of indium chloride anion complexes with TOA. In aqueous HCl solution (0.1-10 M), In3+ exists as octahedral anion complexes, [InCln(H2 O)6-n ]3-n (n = 4); the [InCl6 ]3- complex is dominant at 10 M HCl. The extraction of In3+ from HCl solution with TOA was performed using two kinds of diluents: nitrobenzene (NB) or n-dodecane (DD), which contained 20 vol% of 2-ethylhexanol as an additive. The stoichiometric composition of the extracted complexes, which is estimated from the distribution ratios of In3+ , is affected by the diluents and the HCl concentration of the aqueous phase; the apparent values of TOA/In3+ in the extracted complex are 3 for DD-1 M HCl (diluent-aqueous phase) and DD-5 M HCl, 2 for NB-1 M HCl and NB-5 M HCl, and 1 for NB-10 M HCl. The EXAFS analysis of these extracted complexes shows that the In3+ has ~4 Cl- at ~2.336 Å and no H2 O in the inner coordination sphere; additionally, the shape of the XANES suggests that their coordination geometry is tetrahedral. Therefore, the same tetrahedral [InCl4 ]- complex is formed during the extraction in spite of the variation in the stoichiometric composition (TOA/In3+ = 1-3) of the extracted complexes. [ABSTRACT FROM AUTHOR]- Published
- 2014
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24. SSCP Screening of the Dihydropyrimidine Dehydrogenase Gene Polymorphisms of the Japanese Population Using a Semi-automated Electrophoresis Unit.
- Author
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Yoshihiro Okamoto, Akihito Ueta, Satoshi Sumi, Tetsuya Ito, Yumiko Okubo, Yuki Jose, Akiko Ninomiya, Hajime Togari, and Mikio Nishida
- Subjects
- *
DIHYDROPYRIDINE , *GENETIC polymorphisms , *ELECTROPHORESIS , *ELECTROCHEMISTRY - Abstract
Abstract The single-strand conformation polymorphism (SSCP) procedure has been applied in routine testing for hereditary diseases. Temperature, running buffer, gel composition, and fragment length can influence its sensitivity. Mutation detection in the clinical setting depends on the development of automated technology, especially for large genes, such as the dihydropyrimidine dehydrogenase (DPYD) gene, which codes the initial, rate-limiting enzyme in the catabolism of 5-fluorouracil (5FU). The authors have optimized the condition of SSCP with an automated system (GenePhor system, GE Healthcare UK Ltd.) to screen genetic polymorphisms in the DPYD gene. The efficiency of the method was evaluated using 21 positive controls (DNA samples with polymorphisms in the DPYD gene, previously characterized) and DNA samples from 35 Japanese. Results showed that the use of three different running buffers (pH 7.4, 8.3, and 9.0) in combination with other optimized conditions (10% polyacrylamide gel, 60–90 min at constant 900 V at 5�C) resulted in a high polymorphism detection rate (95.3%), which was considered appropriate for routine screening. Therefore, this strategy could be useful for pharmacogenetic studies on 5FU. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
25. Signal processing technologies for high-density digital magnetic recording.
- Author
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Hisaki Osawa, Yoshihiro Okamoto, and Hidetoshi Saito
- Subjects
- *
SIGNAL processing , *MAGNETIC recorders & recording , *DIGITAL electronics , *CODING theory , *ESTIMATION theory , *ELECTRIC distortion - Abstract
Recently, the demand for higher density digital recording has been increasing steadily. In this article, focusing on digital magnetic recording devices, such as magnetic disks and digital VTRs, a signal processing technique for achieving higher densities is described. First, the partial response system, Viterbi decoding method, and partial response maximum likelihood (PRML) system are described in detail. Next, various signal processing methods extensively studied in recent years for longitudinal magnetic recording are described. Recent research trends in signal processing methods responsive to nonlinear distortions, with allowance for nonlinear distortions, such as partial erasure as factors promoting error rate degradation in high-density recording, are discussed. The performances of various methods are compared. A signal processing method for vertical magnetic recording expected to be a promising technique for future high density recording is described. ©1999 Scripta Technica, Electron Comm Jpn Pt 2, 82(6): 25–45, 1999 [ABSTRACT FROM AUTHOR]
- Published
- 1999
- Full Text
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26. Construction and performance evaluation of a rate 8/10 MSN code for periodically time-varying trellis diagram.
- Author
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Hidetoshi Saito, Takafumi Nishikawa, Yoshihiro Okamoto, and Hisashi Osawa
- Subjects
MAGNETIC recorders & recording ,DECODERS (Electronics) ,CODING theory ,ELECTRONICS ,DATA tapes ,MAGNETIC tapes - Abstract
A conventional 8/10 MSN code was constructed so as not to generate a quasi-catastrophic sequence in the decoder trellis diagram, and needing sliding block decoders. In this article, a new 8/10 MSN code is designed by using a periodically time-varying trellis diagram. The proposed code has an improved squared Euclidean distance distribution between PR output sequences and an improved number of paired codewords that have minimum squared free Euclidean distance decreases as compared with the conventional code. Further, this code can be decoded simply by referring codeword sequences corresponding to data sequences in a look-up table, needing no sliding block decoder. As a result, the proposed 8/10 MSN-coded PR4ML system can provide about 0.8 dB SNR gain over the conventional system at a bit error rate of 10
–4 and a normalized linear density of 3. © 1999 Scripta Technica, Election Comm Jpn Pt 2, 82(3): 31–40, 1999 [ABSTRACT FROM AUTHOR]- Published
- 1999
- Full Text
- View/download PDF
27. ITI canceller for reading shingle-recorded tracks
- Author
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Yoshihiro Okamoto, Hisashi Osawa, Yasuaki Nakamura, Kazumasa Ozaki, and Hiroki Muraoka
- Subjects
Computer science ,Track (disk drive) ,Shingled magnetic recording ,Acoustics ,Reading (computer) ,ITI canceller ,Recording system ,Physics and Astronomy(all) ,Interference (communication) ,Waveform ,Inter-track interference ,Perpendicular magnetic recording ,PRML channel ,Communication channel - Abstract
Shingled magnetic recording attracts much attention as a new recording system for hard disk drive to make the narrower track than the writer-track-width. However, the issue for reading by a wider reader than the track width on the media remains, and the inter-track interference (ITI) from adjacent tracks causes the performance deterioration. To recover the performance, in this paper, we propose an ITI canceller for the reproduced waveform from shingle-recorded tracks, and evaluate the performance of the PRML channel.
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28. Comparison of slicing-induced damage in hexagonal SiC by wire sawing with loose abrasive, wire sawing with fixed abrasive, and electric discharge machining.
- Author
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Yukari Ishikawa, Yong-Zhao Yao, Yoshihiro Sugawara, Koji Sato, Yoshihiro Okamoto, Noritaka Hayashi, Benjamin Dierre, Kentaro Watanabe, and Takashi Sekiguchi
- Abstract
Crystal damage induced in hexagonal SiC by cutting was characterized by transmission electron microscopy and Raman scattering. Wiresawing with loose abrasive (WSLA) induces stacking faults (SFs), dispersive triangular crystal disordered areas, and dislocation half-loop bundles. Wiresawing with fixed abrasive (WSFA) induces SFs, crystal disordered layers, and dislocation half-loop bundles. Electric discharge machining (EDM) predominantly forms silicon, carbon, and 3C-SiC by 6H-SiC decomposition. The mechanisms inducing crystal damage by slicing were discussed on the basis of characterization results. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
29. Effect of 10 MeV iodine ion irradiation on the magnetic properties and lattice structure of CeO2.
- Author
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Takanori Kishino, Ryoichi Shinoda, Koki Shimizu, Yuichi Saitoh, Norito Ishikawa, Yoshihiro Okamoto, Fuminobu Hori, Toshiyuki Matsui, and Akihiro Iwase
- Abstract
We have studied the magnetic properties and the lattice structure of pure CeO
2 irradiated with swift heavy ions. Experimental results showed that the ferromagnetism was induced at room temperature by 10 MeV I ion irradiation. The value of saturation magnetization increases with increasing the ion fluence and reaches a maximum value at about the fluence of 1.2 × 1013 /cm2 , and then decreases. The X-ray diffraction (XRD) spectra showed that the lattice parameter of CeO2 increases with increasing ion fluence. To examine the origin of the ferromagnetic state in CeO2 , we compared the result for 10 MeV I ion irradiation with that for 200 MeV Xe ion irradiation. We also estimated the magnetic properties for CeO2 pellets annealed at 1273 K in a vacuum. From the experimental results, we concluded that oxygen vacancies, which are produced by electronic excitation process due to high energy ion beam, play an important role in the appearance of the ferromagnetic state in CeO2 . [ABSTRACT FROM AUTHOR]- Published
- 2014
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30. A novel ligand based dual conjugate adsorbent for cobalt(II) and copper(II) ions capturing from water.
- Author
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Md. Rabiul Awual, Tsuyoshi Yaita, and Yoshihiro Okamoto
- Subjects
- *
LIGANDS (Chemistry) , *SORBENTS , *COBALT compounds , *COPPER ions , *WATER , *NANOTECHNOLOGY - Abstract
Organic-inorganic based conjugate materials are important in nanotechnology based on the high functionality and stable complex formation with target metal ions at optimum conditions. In this study, a new ligand immobilization based dual and tunable conjugate adsorbent was investigated under specific solution acidity for detection and removal of Co(II) and Cu(II) ions. In this detection system, notable color changes (intense π-π transition) and absorbance spectral intensity of the conjugate adsorbent for Co(II) and Cu(II) were observed at pH 8.0 and 5.2, respectively. Under the optimum conditions, the calibration graphs were the linear and detection limit which were 0.52 and 0.41 μg/L for Co(II) and Cu(II), respectively. The removal efficiency was carried out as a function of solution pH, initial metal ions concentration, and co-existing diverse ions. Sorption equilibrium was well fitted to Langmuir isotherms and determined sorption capacity was 205.33 and 199.20 mg/g for Co(II) and Cu(II), respectively. The adsorbent proved to have an efficient ability for continuous monitoring and removal of Co(II) and Cu(II) ions, even on-site and in situ chemical analyses. The adsorbent was eluted with dilute HCl acid and simultaneously regenerated into its original form. However, several cycles were reused with slight decrease in sorption capacity compared with the initial capacity. Therefore the conjugate adsorbent can be used as cost-effective materials and is a potential candidate for the selective detection and removal of Co(II) and Cu(II) ions from wastewater. The proposed adsorbent was compared with other materials and applied to the analysis and removal of Co(II) and Cu(II) ions from several water samples with satisfactory results. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
31. Selective cesium removal from radioactive liquid waste by crown ether immobilized new class conjugate adsorbent.
- Author
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Awual, Md. Rabiul, Tsuyoshi Yaita, Tomitsugu Taguchi, Hideaki Shiwaku, Shinichi Suzuki, and Yoshihiro Okamoto
- Subjects
- *
RADIOACTIVE contamination , *SORBENTS , *WASTEWATER treatment , *IONIC strength , *MESOPOROUS materials , *SILICA - Abstract
Conjugate materials can provide chemical functionality, enabling an assembly of the ligand complexation ability to metal ions that are important for applications, such as separation and removal devices. In this study, we developed ligand immobilized conjugate adsorbent for selective cesium (Cs) removal from wastewater. The adsorbent was synthesized by direct immobilization of dibenzo-24-crown-8 ether onto inorganic mesoporous silica. The effective parameters such as solution pH, contact time, initial Cs concentration and ionic strength of Na and K ion concentrations were evaluated and optimized systematically. This adsorbent was exhibited the high surface area-to-volume ratios and uniformly shaped pores in case cavities, and its active sites kept open functionality to taking up Cs. The obtained results revealed that adsorbent had higher selectivity toward Cs even in the presence of a high concentration of Na and K and this is probably due to the Cs-π interaction of the benzene ring. The proposed adsorbent was successfully applied for radioactive Cs removal to be used as the potential candidate in Fukushima nuclear wastewater treatment. The adsorbed Cs was eluted with suitable eluent and simultaneously regenerated into the initial form for the next removal operation after rinsing with water. The adsorbent retained functionality despite several cycles during sorption-elution-regeneration operations. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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