1. Stem cell behaviors on periodic arrays of nanopillars analyzed by high-resolution scanning electron microscope images
- Author
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Jihun Kang, Eun-Hye Kang, Young-Shik Yun, Seungmuk Ji, In-Sik Yun, and Jong-Souk Yeo
- Subjects
Cell behavior ,Human adipose-derived stem cell ,Scanning electron microscope ,Polyurethane acrylate ,Nanopillar ,Microscopy ,QH201-278.5 - Abstract
Abstract The biocompatible polyurethane acrylate (PUA) nanopillars were fabricated by soft lithography using three different sizes of nanobeads (350, 500, and 1000 nm), and the human adipose-derived stem cells (hASCs) were cultured on the nanopillars. The hASCs and their various behaviors, such as cytoplasmic projections, migration, and morphology, were observed by high resolution images using a scanning electron microscope (SEM). With the accurate analysis by SEM for the controlled sizes of nanopillars, the deflections are observed at pillars fabricated with 350- and 500-nm nanobeads. These high-resolution images could offer crucial information to elucidate the complicated correlations between nanopillars and the cells, such as morphology and cytoplasmic projections. more...
- Published
- 2020
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