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To examine the utilization of maize stovers (MS) and maize cobs (MC) as replacement of wheat straw (WS) in Total Mixed Ration (TMR) for buffalo calves, 18 calves (aged 14±2 months weighing 189±15 kg) were randomly divided into six equal groups. Six TMRs [iso-nutritious with 12% CP and 68% TDN] having crop residues MS, MC and WS each supplemented with 75% and 65% concentrate (C) were formulated as: T1) MS to C ratio of 25:75%, T2) MS to C ratio of 35:65%, T3) MC to C ratio of 25:75%, T4) MC to C ratio of 35:65%, T5) WS to C ratio of 25:75% and T6) WS to C ratio of 35:65%. These TMRs were offered ad libitum along-with 5 kg green fodder to each calf for 100 days. Daily feed intake, body weight gain and nutrient digestibility during last 5-days were recorded of individual animals. The calves fed TMR with 25% MC attained significantly higher weight gain (830 g/d, P<0.05), followed by the calves fed TMR with 35% MC (760 g/d) whereas, those fed TMR with 35% MS got lowest weight gain (687 g/d). Similarly, better feed conversion ratio (6.63) was observed in calves fed 25% MC based TMR while poor (7.90) FCR was observed in TMR with 35% WS. Intake of calves for dry matter (DM), crude protein (CP), neutral detergent fibre (NDF) and acid detergent fibre (ADF), among all the groups was similar, however, except ADF, digestibility of DM, CP and NDF was significantly (P<0.05) higher in calves fed TMR with 25% MC and lower in those fed 35% WS based TMR. Cost of feed per kg gain was lowest (Rs 128) in calves fed TMR with 35% MC and it was highest (Rs. 167) in both TMRs with 25% WS and 35% WS, respectively. In conclusion, TMR with 35% MC is better in terms of feed cost per kg gain in buffalo calves compared to other rations used in this study. [ABSTRACT FROM AUTHOR]

This study was designed to characterize N gene sequences of peste des petits ruminants virus (PPRV) isolates circulating in Pakistan and to evaluate the efficacy of available diagnostic assays on local isolates. During the study period, a total of sixty PPR outbreaks were investigated. A total of 20 selected samples from these outbreaks were sequenced for N gene. The result analysis and the phylogenetic trees indicated two different viral groups in N gene: one was closer to China and Tajikistan, while other group was similar to isolates from Iran and Saudi Arabia. Efficacy of three commercially available tests for the antigen detection of PPR, that is, peste test, enzyme‐linked immunosorbent assay (ELISA) and reverse transcriptase‐polymerase chain reaction (RT‐PCR) was compared. Keeping PCR as gold standard, sensitivity was calculated as 85% and 57% and specificity was calculated as 83% and 79% for ELISA and peste test, respectively. Value of K for ELISA was 0.67 which indicates good agreement between ELISA and RT‐PCR. Value of K for peste test was 0.33 which indicates fair agreement between peste test and RT‐PCR. In conclusion, study provides premier information about the use of different diagnostic tests and molecular situation of PPRV in Pakistan. [ABSTRACT FROM AUTHOR]

Aflatoxin M1 contamination of milk in Pakistan, like many developing countries, is poorly understood. The present study was therefore conducted to determine AFM1 contamination of milk and its contributory factors in Pakistan. We sampled milk and feedstuffs from 450 peri-urban dairy farms in seven major cities following a cross-sectional study design. Analysis of milk using ELISA revealed high contamination with an overall average of 3164.5 ng of AFM1/L, and significant differences (p < 0.001) between cities. The milk sampled from Gilgit, in northern hilly areas, had an average AFM1 level of 92.5 ng/L. Milk from other cities had 3529.7 ng/L average contamination, with only 5.7% samples qualifying the maximum tolerable limit of 500 ng of AFM1/L. Heavy mean aflatoxin contamination was found in bakery waste (724.6 μg/kg), and cottonseed cake (600.8 μg/kg). Rest of the other feedstuffs had moderate to low mean aflatoxin contamination, ranging from 66.0 μg/kg in maize stover to 3.4 μg/kg in wheat bran. The mean aflatoxin level in commercial dairy concentrates was 32.7 µg/kg. About 80% of the total aflatoxin intake of dairy animals was contributed by cottonseed cake alone due to its high aflatoxin contamination and proportion in dairy rations. On-farm storage time of oilseed cakes varied (p < 0.01) in different cities but was not associated with aflatoxin contamination. The exceptionally high AFM1 contamination suggests that milk from peri-urban dairy farms is a serious public health threat in Pakistan. This situation can be mitigated by reducing aflatoxin contamination in cottonseed cake and promoting the use of commercial concentrates and other feedstuffs with low contamination. [ABSTRACT FROM AUTHOR]

Hog deer (Axis porcinus) are an endangered species in Pakistan, and there is little information about their feed preference in captivity. Seven animals were offered weighed amount of feed in two seasonal binary combinations of fresh fodder and one combination of grains. The fresh seasonal fodder included Oat (Avena sativa), Lucerne (Medicago sativa), Sorghum (Sorghum bicolor), and Maize (Zea mays); whereas grains included Wheat (Triticum aestivum) whole-grains and Chickpea (Cicer arietinum) seeds. Thirteen plant samples were collected from the wild habitat of Hog deer in Tunsa Barrage Wildlife Sanctuary. Plant samples from wild and captivity were analysed through proximate analysis for Crude protein, Fiber, Fat and Ash contents. The outcomes concluded that in combination C1, the hog deer preferred Oat over Lucerne (p < 0.05) with a mean percentage±standard error (Mean±SE) of 90.757±0.401 percent for Oat and 9.243±0.401 percent for Lucerne. The animals preferred Maize (p < 0.05) in combination C2 with mean percentage and standard error of 91.657±0.368 percent compared to Sorghum with mean percentage and standard error of 8.343±3.083 percent. Chickpea was the preferred feed item (p < 0.05) against Wheat from the C3 combination with mean percentages and standard error of 92.2±0.453 percent and 7.800±0.453 percent respectively. The results of the proximate analysis revealed that the percentage of Crude Protein was 15.69±4.8 percent and 12.63±1.4 percent for plants in Captivity and plants in the wild respectively. The percentages of Fat content in plants in captivity and the wild were 3.34±0.86 and 3.37±0.41 respectively. The results showed percentages of Ash content in plants both in captive and wild,10.9±5.9 and 32.83±0.98 respectively; whereas, the percentages of fibre contents in the plants in captivity and wild habitats were 21.00±3.9 and 27.01±2.6 respectively. [ABSTRACT FROM AUTHOR]

Background: ELISA is a widely used method for aflatoxin M1 (AFM1) quantification in dairy products. This study was conducted to compare ELISA kits from different manufacturers for AFM1 quantification in milk. Methods: High sensitivity ELISA kits (for up to 250 ng AFM1/L), including AgraQuant (Romer Labs), Bioshield M1ES (Prognosis Biotech), Helica 96 (Helica Biosystems), Veratox (Neogen, Inc.), and the medium sensitivity kit Immunolab AM1E01 (Immunolab GmbH; for 10–1000 ng AFM1/L) were tested against a certified reference AFM1 whole milk having 44 ng AFM1/L and its 10-fold dilution, and a 50 ng AFM1/L standard. In another experiment, Prognosis Bioshield M1UF (70–1000 ng AFM1/L), Romer’s AgraquantPlus (10–2000 ng AFM1/L), and Immunulab’s AM1E01 kits were compared to test a 500 ng AFM1/L solution. Results: In both of the experiments, the quantification of the tested AFM1 levels did not differ (P ≥ 0.310) between the kits from various manufacturers. In the case of 4.4 ng AFM1/L, recovery of the toxin was close to the assigned value under the kit by Neogen, Inc. In case the of 44 ng level, better recoveries were seen under the kits by Immunolab and Prognosis Biotech. In both the cases, low relative standard deviation (RSD) values were obtained only for the kit by Prognosis Biotech. In the case of the 50 and 500 ng AFM1/L solutions, the kits by Prognosis Biotech and Romer Labs provided recoveries close to the assigned values as well as low RSD. Conclusions: These data indicate that all the studied ELISA kit brands had comparable efficacy for AFM1 quantification. Recovery of the toxin and RSD may, however, differ between kits from various manufacturers. [ABSTRACT FROM AUTHOR]

Abstract: Little is known about the effects of ethanol on gastrointestinal tract of chicken. In this study, we investigated the effects of low levels of ethanol on electrophysiological variables of jejunal epithelium of commercial broilers. Jejunal tissues from 35- to 39-day-old broilers were exposed to either 0 or 0.1% ethanol in Ussing chambers, and electrophysiological variables were monitored for 40min. After 40 and 60min of incubation, glucose (20mM) and carbamoylcholine (200μM), respectively, were introduced into the chambers. The absolute and percent increase in short-circuit current (I sc) and potential difference (V t) induced by glucose were increased significantly with 0.1% ethanol. There was no significant effect of 0.1% ethanol on carbamoylcholine-induced electrophysiological variables. To investigate if higher levels of ethanol have similar effects, we tested the effects of 0, 0.33, and 0.66% ethanol under similar experimental conditions until the glucose-addition step. Contrary to 0.1% ethanol, both the 0.33 and 0.66% ethanol levels significantly decreased the basal and glucose-induced I sc and V t. Tissue conductivity remained unaffected in all cases. These results indicate that intestinal epithelia of chicken may be more sensitive to the effects of ethanol as compared with other species. This is the first report indicating dose-dependent increase and decrease in active glucose absorption in intestinal epithelia in the presence of ethanol. [Copyright &y& Elsevier]

A longitudinal one-year study was conducted to determine aflatoxin M1 levels in different types of milk marketed in Pakistan. Processed and raw liquid milk from 21 sources, two milk powder and six tea whitener brands were sampled on monthly basis from Islamabad. The aflatoxin M1 levels in liquid milk were lower (p < 0.05) in summer (April to July) compared with the levels in winter (January, November and December). The mean aflatoxin M1 levels were 254.9, 939.5, and 1535.0 ng/L in UHT, pasteurized, and raw milk, respectively (differing at p < 0.001). The mean toxin level in powdered milk after reconstitution was 522.1 ng/L. Overall, 12.9, 41.0, 91.9 and 50.0% of the UHT, pasteurized, raw and powdered milk samples, respectively, exceeded the Codex maximum tolerable limit of 500 ng of aflatoxin M1/L. It was estimated that consumers of raw and processed milk were exposed to 11.9 and 4.5 ng aflatoxin M1, respectively, per kg of body weight daily. The study indicates potential aflatoxin M1 exposure risks for the consumers of raw milk in the country. The levels of the toxin though comparatively lower in milk powder, requires attention as this type of milk is consumed by infants. [ABSTRACT FROM AUTHOR]

Monitoring of aflatoxin levels in milk is often complicated in developing countries due to the dominance of informal markets channeling milk in raw form. Farmer's awareness and voluntary participation in aflatoxin mitigation can be critical in such scenarios. Therefore, the present study was conducted to understand the perceptions of dairy farmers about aflatoxins and link it with aflatoxin mitigation programs on milk in Pakistan. Information was collected from 450 peri-urban dairy farmers in seven cities using questionnaires. Majority (77.9%) of the farmers were aware of the negative impact of moldy feed on animal health. However, only 40.6% of the farmers were aware of the transferability of the toxins from moldy feed to milk. The farmers had almost no awareness of aflatoxins as 95% never heard of the term. After receiving an onsite briefing on effects of the toxin on animal and human health, and its transferability to milk, 98.3% farmers showed willingness to buy aflatoxin-safe feedstuffs, while 88.5% showed willingness to control aflatoxin in milk. Around half of the farmers considered aflatoxin control programs as affordable. On average, farmers agreed to pay 10.1% higher price for aflatoxin certified oilseed cakes. Availability of feedstuffs certified of low aflatoxin content was suggested by 22% of the participants as the critical step in reducing aflatoxins in milk. Other important suggestions included; subsidy on quality feeds (18%), raising awareness (18%), and legislation and monitoring (16%). The present results suggest that the current practice of milk monitoring in the country can yield desirable results only if it is coupled with feed certification programs ensuing availability of aflatoxin-safe feeds. Further, awareness can positively impact participation of producers in aflatoxin control programs. In this regard, awareness about effects of aflatoxins on animal health was found to be a more powerful trigger of voluntary control compared with the awareness of the toxin's transferability to milk., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Yunus, Lindahl, Anwar, Ullah and Ibrahim.)

Background: ELISA is a widely used method for aflatoxin M 1 (AFM 1 ) quantification in dairy products. This study was conducted to compare ELISA kits from different manufacturers for AFM 1 quantification in milk. Methods: High sensitivity ELISA kits (for up to 250 ng AFM 1 /L), including AgraQuant (Romer Labs), Bioshield M1ES (Prognosis Biotech), Helica 96 (Helica Biosystems), Veratox (Neogen, Inc.), and the medium sensitivity kit Immunolab AM1E01 (Immunolab GmbH; for 10-1000 ng AFM 1 /L) were tested against a certified reference AFM 1 whole milk having 44 ng AFM 1 /L and its 10-fold dilution, and a 50 ng AFM 1 /L standard. In another experiment, Prognosis Bioshield M1UF (70-1000 ng AFM 1 /L), Romer's AgraquantPlus (10-2000 ng AFM 1 /L), and Immunulab's AM1E01 kits were compared to test a 500 ng AFM 1 /L solution. Results: In both of the experiments, the quantification of the tested AFM 1 levels did not differ ( P ≥ 0.310) between the kits from various manufacturers. In the case of 4.4 ng AFM 1 /L, recovery of the toxin was close to the assigned value under the kit by Neogen, Inc. In case the of 44 ng level, better recoveries were seen under the kits by Immunolab and Prognosis Biotech. In both the cases, low relative standard deviation (RSD) values were obtained only for the kit by Prognosis Biotech. In the case of the 50 and 500 ng AFM 1 /L solutions, the kits by Prognosis Biotech and Romer Labs provided recoveries close to the assigned values as well as low RSD. Conclusions: These data indicate that all the studied ELISA kit brands had comparable efficacy for AFM 1 quantification. Recovery of the toxin and RSD may, however, differ between kits from various manufacturers.