8 results on '"Zeng, Haipan"'
Search Results
2. A transcriptomic analysis of superhybrid rice L YP9 and its parents
- Author
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Wei, Gang, Tao, Yong, Liu, Guozhen, Chen, Chen, Luo, Renyuan, Xia, Hongai, Gan, Qiang, Zeng, Haipan, Lu, Zhike, Han, Yuning, Li, Xiaobing, Song, Guisheng, Zhai, Hongli, Peng, Yonggang, Li, Dayong, Xu, Honglin, Wei, Xiaoli, Cao, Mengliang, Deng, Huafeng, Xin, Yeyun, Fu, Xiqin, Yuan, Longping, Yu, Jun, Zhu, Zhen, and Zhu, Lihuang
- Subjects
Hybrid rice -- Properties ,Quantitative trait loci -- Identification and classification ,Science and technology - Abstract
By using a whole-genome oligonucleotide microarray, designed based on known and predicted indica rice genes, we investigated transcriptome profiles in developing leaves and panicles of superhybrid rice LYP9 and its parental cultivars 93-11 and PA64s. We detected 22,266 expressed genes out of 36,926 total genes set collectively from 7 tissues, including leaves at seedling and tillering stages, flag leaves at booting, heading, flowering, and filling stages, and panicles at filling stage. Clustering results showed that the F1 hybrid's expression profiles resembled those of its parental lines more than that which lies between the 2 parental lines. Out of the total gene set, 7,078 genes are shared by all sampled tissues and 3,926 genes (10.6% of the total gene set) are differentially expressed genes (DG). As we divided DG into those between the parents ([DG.sub.pp]) and between the hybrid and its parents ([DG.sub.HP]), the comparative results showed that genes in the categories of energy metabolism and transport are enriched in [DG.sub.HP] rather than in [DG.sub.pp]. In addition, we correlated the concurrence of DG and yield-related quantitative trait loci, providing a potential group of heterosisrelated genes. heterosis | hybrid rice | transcriptorne | quantitative trait loci | differentially expressed genes
- Published
- 2009
3. Profiling Caenorhabditis elegans non-coding RNA expression with a combined microarray
- Author
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He, Housheng, Cai, Lun, Skogerbø, Geir, Deng, Wei, Liu, Tao, Zhu, Xiaopeng, Wang, Yudong, Jia, Dong, Zhang, Zhihua, Tao, Yong, Zeng, Haipan, Aftab, Muhammad Nauman, Cui, Yan, Liu, Guozhen, and Chen, Runsheng
- Published
- 2006
4. Transcriptional Characteristics of Xa21-mediated Defense Responses in Rice.
- Author
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Gan, Qiang, Bai, Hui, Zhao, Xianfeng, Tao, Yong, Zeng, Haipan, Han, Yuning, Song, Wenyuan, Zhu, Lihuang, and Liu, Guozhen
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XANTHOMONAS diseases ,BACTERIAL diseases of plants ,RICE diseases & pests ,PLANT clones ,CELLULAR signal transduction ,MOLECULAR genetics ,TRANSCRIPTION factors ,GENETIC transcription ,PLANT defenses ,RICE genetics - Abstract
Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is the most destructive bacterial disease of rice. The cloned rice gene Xa21 confers resistance to a broad spectrum of Xoo races. To identify genes involved in Xa21‐mediated immunity, a whole‐genome oligonucleotide microarray of rice was used to profile the expression of rice genes between incompatible interactions and mock treatments at 0, 4, 8, 24, 72 and 120 h post inoculation (hpi) or between incompatible and compatible interactions at 4 hpi, respectively. A total of 441 differentially expressed genes, designated as XDGs (Xa21 mediated differentially expressed genes), were identified. Based on their functional annotations, the XDGs were assigned to 14 categories, including defense‐related, signaling, transcriptional regulators. Most of the defense‐related genes belonged to the pathogenesis‐related gene family, which was induced dramatically at 72 and 120 hpi. Interestingly, most signaling and transcriptional regulator genes were downregulated at 4 and 8 hpi, suggesting that negative regulation of cellular signaling may play a role in the Xa21‐mediated defense response. Comparison of expression profiles between Xa21‐ and other R gene‐mediated defense systems revealed interesting common responses. Representative XDGs with supporting evidences were also discussed. [ABSTRACT FROM AUTHOR]
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- 2011
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- View/download PDF
5. A protein chip approach for high-throughput antigen identification and characterization.
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Hu, Shaohui, Li, Yu, Liu, Guozhen, Song, Qifeng, Wang, Li, Han, Yuning, Zhang, Yang, Song, Yali, Yao, Xiying, Tao, Yong, Zeng, Haipan, Yang, Huanming, Wang, Jian, Zhu, Heng, Chen, Zhi-Nan, and Wu, Lin
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- 2007
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6. Novel systemic lupus erythematosus autoantigens identified by human protein microarray technology
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Huang, Wei, Hu, Chaojun, Zeng, Haipan, Li, Ping, Guo, Lei, Zeng, Xiaofeng, Liu, Guozhen, Zhang, Fengchun, Li, Yongzhe, and Wu, Lin
- Subjects
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SYSTEMIC lupus erythematosus , *AUTOANTIGENS , *PROTEIN microarrays , *AUTOIMMUNE diseases , *AUTOANTIBODIES , *HUMAN proteins , *SJOGREN'S syndrome , *VENEZUELAN equine encephalomyelitis - Abstract
Abstract: Systemic lupus erythematosus (SLE) is a systemic autoimmune disease affecting many organs. Many autoantibodies have been associated with the disease, but either in low specificity or low sensitivity of detection. In an aim to screen for better autoantibodies, we profiled the autoantibody repertoire in sera from 30 SLE patients versus 30 healthy controls using a protein microarray containing 5011 non-redundant human proteins, and identified four candidates. We then selected CLIC2 for further verification by ELISA in an extended cohort including 110 SLE, 121 non-AD, 118 RA, 117 SSc, and 105 pSS patients. The positive rate of anti-CLIC2 was 28.18% in SLE patients, significantly higher than those in non-AD, RA, and SSc patients. The presence of anti-CLIC2 in SLE had positive correlation with disease activity in terms of SLEDAI score and several indexes (p <0.05). [Copyright &y& Elsevier]
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- 2012
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7. Novel autoimmune hepatitis-specific autoantigens identified using protein microarray technology.
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Song Q, Liu G, Hu S, Zhang Y, Tao Y, Han Y, Zeng H, Huang W, Li F, Chen P, Zhu J, Hu C, Zhang S, Li Y, Zhu H, and Wu L
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- Algorithms, Autoantigens chemistry, Biomarkers blood, Biomarkers chemistry, DNA, Complementary genetics, DNA, Complementary metabolism, Equipment Design, Hepatitis, Autoimmune immunology, Humans, Liver metabolism, Logistic Models, Protein Array Analysis instrumentation, Proteins genetics, Recombinant Proteins genetics, Recombinant Proteins metabolism, Reproducibility of Results, Autoantigens blood, Hepatitis, Autoimmune blood, Protein Array Analysis methods, Proteins metabolism
- Abstract
Autoimmune hepatitis (AIH) is a chronic necroinflammatory disease of the liver with a poorly understood etiology. Detection of nonorgan-specific and liver-related autoantibodies using immunoserological approaches has been widely used for diagnosis and prognosis. However, unambiguous and accurate detection of the disease requires the identification and characterization of disease-specific autoantigens. In the present study, we have profiled the autoantigen repertoire of patients with AIH versus those with other liver diseases, identifying and validating three novel and highly specific biomarkers for AIH. In phase I, we fabricated a human protein chip of 5011 nonredundant proteins and used it to quickly identify 11 candidate autoantigens with relative small serum collection. In phase II, we fabricated an AIH-specific protein chip and obtained autoimmunogenic profiles of serum samples from 44 AIH patients, 50 healthy controls, and 184 additional patients suffering from hepatitis B, hepatitis C, systemic lupus erythematosus, primary Sjogren's syndrome, rheumatoid arthritis, or primary biliary cirrhosis. With this two-phase approach, we identified three new antigens, RPS20, Alba-like, and dUTPase, as highly AIH-specific biomarkers, with sensitivities of 47.5% (RPS20), 45.5% (Alba-like), and 22.7% (dUTPase). These potential biomarkers were further validated with additional AIH samples in a double-blind design. Finally, we demonstrated that these new biomarkers could be readily applied to ELISA-based assays for use in clinical diagnosis/prognosis.
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- 2010
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8. The structure analysis and antigenicity study of the N protein of SARS-CoV.
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Wang J, Ji J, Ye J, Zhao X, Wen J, Li W, Hu J, Li D, Sun M, Zeng H, Hu Y, Tian X, Tan X, Xu N, Zeng C, Wang J, Bi S, and Yang H
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- Amino Acid Motifs genetics, Amino Acid Sequence, Base Composition, Base Sequence, Cluster Analysis, Computational Biology, Coronavirus Nucleocapsid Proteins, DNA Primers, Enzyme-Linked Immunosorbent Assay, Molecular Sequence Data, Nucleocapsid Proteins immunology, Nucleocapsid Proteins metabolism, Phosphorylation, Sequence Analysis, DNA, Antigens, Viral immunology, Genetic Variation, Nucleocapsid Proteins genetics, Severe acute respiratory syndrome-related coronavirus genetics
- Abstract
The Coronaviridae family is characterized by a nucleocapsid that is composed of the genome RNA molecule in combination with the nucleoprotein (N protein) within a virion. The most striking physiochemical feature of the N protein of SARS-CoV is that it is a typical basic protein with a high predicted pI and high hydrophilicity, which is consistent with its function of binding to the ribophosphate backbone of the RNA molecule. The predicted high extent of phosphorylation of the N protein on multiple candidate phosphorylation sites demonstrates that it would be related to important functions, such as RNA-binding and localization to the nucleolus of host cells. Subsequent study shows that there is an SR-rich region in the N protein and this region might be involved in the protein-protein interaction. The abundant antigenic sites predicted in the N protein, as well as experimental evidence with synthesized polypeptides, indicate that the N protein is one of the major antigens of the SARS-CoV. Compared with other viral structural proteins, the low variation rate of the N protein with regards to its size suggests its importance to the survival of the virus.
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- 2003
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