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At present, anti-counterfeiting schemes based on the combination of anti-counterfeiting patterns and two-dimensional codes is a research hotspot in digital anti-counterfeiting technology. However, many existing identification schemes rely on special equipment such as scanners and microscopes; there are few methods for authentication that use smartphones. In particular, the ability to classify blurry pattern images is weak, leading to a low recognition rate when using mobile terminals. In addition, the existing methods need a sufficient number of counterfeit patterns for model training, which is difficult to acquire in practical scenarios. Therefore, an authentication scheme for an anti-counterfeiting pattern captured by smartphones is proposed in this paper, featuring a single classifier consisting of two modules. A feature extraction module based on U-Net extracts the features of the input images; then, the extracted feature is input to a one-class classification module. The second stage features a boundary-optimized OCSVM classification method. The classifier only needs to learn positive samples to achieve effective identification. The experimental results show that the proposed approach has a better ability to distinguish the genuine and counterfeit anti-counterfeiting pattern images. The precision and recall rate of the approach reach 100%, and the recognition rate for the blurry images of the genuine anti-counterfeiting patterns is significantly improved. [ABSTRACT FROM AUTHOR]

Print matter authentication based on anti-counterfeiting techniques has received continuously increasing concern from academia and industry. However, the existing printing anti-counterfeiting solutions often have the defects of poor identification experience, high cost, or weak anti-counterfeiting ability, and cannot achieve pre-sale anti-counterfeiting. Therefore, a novel steganography-based pattern for print matter anti-counterfeiting by smartphone cameras is proposed in this study. Firstly, every pixel in the original binary message image (such as QR code) is replaced by a square pixel block with the same binary gray value of 0 or 255 (the first-level expansion). Secondly, the obtained image is encrypted based on the logistic chaotic sequence, and then scrambled by Arnold transform. Lastly, once again every pixel in the generated image is replaced with a square pixel block (the second-level expansion), the size and gray value of which can be set to control the semi-fragile ability to distinguish an originally printed pattern from its illegitimate copy. If the message extracted from the printed pattern through the inverse procedure is complete enough to decode and read, the pattern is assumed to be an original print. Experimental results verify the advancement and effectiveness of the proposed scheme in distinguishing the copied pattern. [ABSTRACT FROM AUTHOR]

The commensal microbiota regulates the self-renewal and differentiation of hematopoietic stem and progenitor cells (HSPCs) in bone marrow. Whether and how the microbiota influences HSPC development during embryogenesis is unclear. Using gnotobiotic zebrafish, we show that the microbiota is necessary for HSPC development and differentiation. Individual bacterial strains differentially affect HSPC formation, independent of their effects on myeloid cells. Early-life dysbiosis in chd8 −/− zebrafish impairs HSPC development. Wild-type microbiota promote HSPC development by controlling basal inflammatory cytokine expression in kidney niche, and chd8 −/− commensals elicit elevated inflammatory cytokines that reduce HSPCs and enhance myeloid differentiation. We identify an Aeromonas veronii strain with immuno-modulatory activities that fails to induce HSPC development in wild-type fish but selectively inhibits kidney cytokine expression and rebalances HSPC development in chd8 −/− zebrafish. Our studies highlight the important roles of a balanced microbiome during early HSPC development that ensure proper establishment of lineal precursor for adult hematopoietic system. [Display omitted] • Microbiota depletion impairs HSPC development during embryogenesis in zebrafish • Disturbed microbiota in chd8 −/− decreases HSPC formation and promotes myelopoiesis • Different strains induce different levels of cytokine expression in HSPC niche • Local cytokine expression controls HSPC formation and myeloid differentiation Zhong et al. show that commensal microbiota regulates normal HSPC development by mediating appropriate inflammatory cytokine expression in HSPC niche. The microbiota of chd8 −/− zebrafish induces enhanced cytokine expression and impairs HSPC development. An Aeromonas strain selectively suppresses excessive cytokines in HSPC niche and restores HSPC development in chd8 −/− zebrafish. [ABSTRACT FROM AUTHOR]

Endocrine disrupting chemicals (EDCs) can act as agonists, antagonists or mixed agonists/antagonists toward estrogen receptor α (ERα) and glucocorticoid receptor (GR) in a tissue- and cell-specific manner. However, the activation/inhibition mechanism by which structurally different chemicals induce various types of disruption remain ambiguous. This unrevealed theory limited the in silico modeling of EDCs and the prioritization of potential EDCs for experimental testing. As a kind of chemical widely used in manufacture, bisphenols (BPs) have attracted great attentions on their potential endocrine disrupting effects. BPs used in this study exhibited pure agonistic, pure antagonistic or mixed agonistic/antagonistic activities toward ERα and/or GR. According to the mechanistic modeling, the pure agonistic and pure antagonistic activities were attributed to a single type of protein conformation induced by BPs-ERα and/or BPs-GR interactions, whereas the mixed agonistic/antagonistic activities were attributed to multiple conformations that concomitantly exist. After interacting with BPs, the active conformation recruits coactivator to induce agonistic activity and the blocked conformation inhibits coactivator to induce antagonistic activity, whereas the concomitantly-existing multiple conformations (active, blocked and competing conformations) recruit coactivator, recruit corepressor and/or inhibit coactivator to dually induce the agonistic and antagonistic activities. Therefore, the in silico modeling in this study can not only predict ERα and GR disrupting activities but also, especially, identify the potential mechanisms. This mechanistic study breaks the current bottleneck of computational toxicology and can be widely used to prioritize potential estrogen/glucocorticoid disruptor for experimental testing in both pre-clinic and clinic studies. Unlabelled Image • The estrogen and glucocorticoid disrupting activities of bisphenols were cell line-dependent. • Molecular mechanisms are related to ER/GR conformations and coregulator recruitment/inhibition. • Mixed agonist/antagonist may result from multiple conformations of ER/GR. • An in silico workflow for prediction of endocrine disruptors was developed. [ABSTRACT FROM AUTHOR]