9 results on '"Zhu, Fen-Xia"'
Search Results
2. Biotransformation of ginsenosides F4 and Rg6 in zebrafish.
- Author
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Shen, Wen-Wen, Zhang, Hai-Xia, Qiu, Shou-Bei, Wei, Ying-Jie, Zhu, Fen-Xia, Wang, Jing, Wang, Dan-Dan, Jia, Xiao-Bin, Tang, Dao-Quan, and Chen, Bin
- Subjects
BIOTRANSFORMATION (Metabolism) ,FISHES ,GINSENG ,GLYCOSIDES ,LIQUID chromatography ,MASS spectrometry ,MOLECULAR structure - Abstract
Ginsenosides F
4 and Rg6 (GF4 and GRg6 ), two main active components of steamed notoginseng or red ginseng, are dehydrated disaccharide saponins. In this work, biotransformation of ginsenosides F4 and Rg6 in zebrafish was investigated by qualitatively identifying their metabolites and then proposing their possible metabolic pathways. The prediction of possible metabolism of ginsenosides F4 and Rg6 using zebrafish model which can effectively simulate existing mammals model was early and quickly performed. Metabolites of ginsenosides F4 and Rg6 after exposing to zebrafish for 24 h were identified by Ultraperformance Liquid Chromatography/Quadrupole-Time-of-Flight Mass Spectrometry. A total of 8 and 6 metabolites of ginsenosides F4 and Rg6 were identified in zebrafish, respectively. Of these, 7 and 5, including M1, M3−M5, M7−M9 and N1 (N5), N2, N4 (N9), N7−N8 were reported for the first time as far as we know. The mechanisms of their biotransformation involved were further deduced to be desugarization, glucuronidation, sulfation, dehydroxylation, loss of C-17 and/or C-23 residue pathways. It was concluded that loss of rhamnose at position C-6 and glucuronidation at position C-3 in zebrafish were considered as the main physiologic and metabolic processes of ginsenosides F4 and ginsenosides Rg6 , respectively. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
3. A novel drug-phospholipid complex loaded micelle for baohuoside I enhanced oral absorption: in vivo and in vivo evaluations.
- Author
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Jin, Xin, Zhang, Zhen-Hai, Sun, E., Tan, Xiao-Bin, Zhu, Fen-Xia, and Jia, Xiao-Bin
- Subjects
PHOSPHOLIPIDS ,MICELLES ,ORAL drug administration ,DRUG absorption ,ANTINEOPLASTIC agents ,TRANSMISSION electron microscopy ,ORGANIC solvents - Abstract
Baohuoside I is an effective anti-cancer drug currently used for a variety of cancers in vitro. Unfortunately, baohuoside I has a very poor solubility in both water and in organic solvents. Besides that, it is subject to significant efflux. This work therefore aimed at evaluating the ability of mixed micelles to solubilize baohuoside I, increase permeability and inhibit efflux of baohuoside I to promote oral absorption. A novel (TPGS-baohuoside I-phospholipid complex) mixed micelles was formed by phospholipid complex and TPGS to increase the solubility, enhance permeability, and inhibit efflux of baohuoside I. Micelle formation was confirmed by differential scanning calorimetry and transmission electron microscopy. The average diameters and efflux ratio of mixed micelles decreased as the ratio of TPGS increased with a respective increase in solubility of baohuoside I. Nevertheless, a slow release of baohuoside I from loaded micelles was noted. The results showed that at a 1:9 ratio for baohuoside I-phospholipid complex: TPGS in mixed micelles, solubility of baohuoside I increased up to 88 fold while the efflux ratio decreased by 85%. Their smaller size and higher zeta potential values indicated that mixed micelles would be easily absorbed and more stable. The relative bioavailability of the micelles (AUC
0-∞ ) compared with baohuoside I (AUC0-∞ ) was 533%, demonstrating great potential for clinical application. Hence, the novel micelles formed with phospholipid complex and TPGS considerably increased drug concentration in the media and enhanced absorption. [ABSTRACT FROM AUTHOR]- Published
- 2013
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4. Corydalis bungeana Turcz. attenuates LPS-induced inflammatory responses via the suppression of NF-κB signaling pathway in vitro and in vivo.
- Author
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Zhai, Xiao-Ting, Chen, Jia-Quan, Jiang, Cui-Hua, Song, Jie, Li, Dong-Yu, Zhang, Hao, Jia, Xiao-Bin, Tan, Wei, Wang, Shu-Xia, Yang, Yi, and Zhu, Fen-Xia
- Subjects
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PROTEIN metabolism , *LIVER analysis , *ALTERNATIVE medicine , *ANIMAL experimentation , *ANTI-inflammatory agents , *BIOLOGICAL models , *CELLULAR signal transduction , *CYTOKINES , *ENZYME-linked immunosorbent assay , *HISTOLOGICAL techniques , *IMMUNOHISTOCHEMISTRY , *INFLAMMATION , *INTERLEUKINS , *MACROPHAGES , *MEDICINAL plants , *MICE , *NITRIC oxide , *PHOSPHORYLATION , *POLYMERASE chain reaction , *TUMOR necrosis factors , *WESTERN immunoblotting , *DNA-binding proteins , *PLANT extracts , *IN vitro studies , *IN vivo studies , *PHARMACODYNAMICS - Abstract
Ethnopharmacological relevance Corydalis bungeana Turcz. ( C. bungeana ) is one of traditionally used medicines in China and possesses various biological effects, such as anti-inflammatory, antibacterial activity and inhibition of the immune function of the host. Aim of the study we studied the anti-inflammatory effect and molecular mechanism involved of C. bungeana both in vitro and in vivo model system in which the inflammatory response was induced by LPS treatment. Materials and methods Anti-inflammatory activity of C. bungeana was investigated by LPS-induced RAW 264.7 macrophages and BALB/c mice. The production and expression of pro-inflammatory cytokines were evaluated by Griess reagent, ELISA kits and RT-qPCR, respectively. Phosphorylation status of IκBα and p65 was illustrated by western blot assay. Results C. bungeana reduced the secretion of NO, TNF-α, IL-6 and IL-1β through inhibiting the protein expression of iNOS, TNF-α, IL-6 and IL-1β in vitro and in vivo . Western blot analysis suggested that C. bungeana supressed NF-κB activation via regulating the phosphorylation of IκBα and p65. Immunohistochemical assay also demostrated the histological inflammatory change in liver tissue. Conclusions The results indicate that C. bungeana supresses the activation of NF-κB signaling pathway through inhibiting phosphorylation of IκBα and p65, which results in good anti-inflammatory effect. In addition, C. bungeana attenuates inflammatory reaction by supressing the expression of various inflammatory cytokines both in vivo and in vitro . [ABSTRACT FROM AUTHOR]
- Published
- 2016
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5. Nauclea officinalis inhibits inflammation in LPS-mediated RAW 264.7 macrophages by suppressing the NF-κB signaling pathway.
- Author
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Zhai, Xiao-Ting, Zhang, Zhi-Yuan, Jiang, Cui-Hua, Chen, Jia-Quan, Ye, Ji-Qing, Jia, Xiao-Bin, Yang, Yi, Ni, Qian, Wang, Shu-Xia, Song, Jie, and Zhu, Fen-Xia
- Subjects
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ANIMAL experimentation , *ANTI-inflammatory agents , *CELL lines , *CELLULAR signal transduction , *ENZYME-linked immunosorbent assay , *HERBAL medicine , *INTERLEUKINS , *MACROPHAGES , *CHINESE medicine , *MICE , *PHOSPHORYLATION , *POLYMERASE chain reaction , *TUMOR necrosis factors , *WESTERN immunoblotting , *DNA-binding proteins , *LIPOPOLYSACCHARIDES , *DRUG administration , *DRUG dosage , *PHARMACODYNAMICS - Abstract
Ethnopharmacological relevance Nauclea officinalis has been traditionally used in China for the treatment of fever, pneumonia and enteritidis etc. This study aims to investigate effects of N. officinalis on the inflammatory response as well as the possible molecular mechanism in LPS-stimulated RAW 264.7 murine macrophage cells. Materials and methods Anti-inflammatory activity of N. officinalis (10, 20, 50, and 100 µg/mL) was investigated by using LPS-induced RAW 264.7 macrophages. The NO production was determined by assaying nitrite in culture supernatants with the Griess reagent. The levels of TNF-α, IL-6 and IL-1β in culture media were measured with ELISA kits. Real time fluorescence quantitative PCR was detected for mRNA expression of iNOS, TNF-α, IL-6 and IL-1β. Western blot assay was performed to illustrate the inhibitory effects of N. officinalis on phosphorylation of IκB-α and NF-κB p65. Results Treatment with N. officinalis (10–100 µg/mL) dose-dependently inhibited the production as well as mRNA expression of NO, TNF-α, IL-6 and IL-1β in RAW 264.7 macrophages. Western blot assay suggested that the mechanism of the anti-inflammatory effect was associated with the inhibition of phosphorylation of IκB-α and NF-κB p65. Conclusions The results indicated that N. officinalis potentially inhibited the activation of upstream mediator NF-κB signaling pathway via suppressing phosphorylation of IκB-α and NF-κB p65 to inhibit LPS-stimulated inflammation. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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6. Effect of medical care linkage-continuous management mode in patients with posterior circulation cerebral infarction undergoing endovascular interventional therapy.
- Author
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Zhu FX and Ye Q
- Abstract
Background: Acute cerebral infarction is a severe type of ischemic stroke that can be divided into anterior circulation cerebral infarction and posterior circulation cerebral infarction (PCCI). PCCI affects the structure of the posterior circulation brain, because posterior part of the brain, which has more complex anatomical structures and more prone to posterior circulation vascular variation. Therefore, improving the prognosis of PCCI patients is necessary., Aim: To explore the effect of medical care linkage-continuous management mode (MCLMM) on endovascular interventional therapy (EIT) for PCCI., Methods: Sixty-nine patients with PCCI who received EIT and conventional nursing intervention were selected as the control group, and 78 patients with PCCI who received EIT and MCLMM intervention were selected as the observation group. The incidence of postoperative complications, compliance and disease self-management behavior after six months of intervention, modified Rankin scale (mRS) and Barthel index (BI) scores in the acute phase and after one year of intervention, and recurrence within one year were compared between the two groups., Results: The total incidence rate of postoperative complications in the observation group (7.69%) was lower than that in the control group (18.84%) ( P < 0.05). The scores for medical compliance behavior (regular medication, appropriate diet, and rehabilitation cooperation rates) and disease self-management behavior (self-will, disease knowledge, and self-care ability) in the observation group were higher than those in the control group ( P < 0.05). After one year of intervention, in the observation group, the mRS score was significantly lower, and the BI score was significantly higher than those in the control group ( P < 0.05). The recurrence rate within one year in the observation group (3.85%) was significantly lower than that in the control group (13.04%) ( P < 0.05)., Conclusion: MCLMM can reduce the incidence of complications after EIT for PCCI, improve patient compliance behavior and disease self-management ability, and promote the recovery of neurological function., Competing Interests: Conflict-of-interest statement: There are no conflicts of interest to report., (©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
7. Biotransformation of ginsenosides F 4 and Rg 6 in zebrafish.
- Author
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Shen WW, Zhang HX, Qiu SB, Wei YJ, Zhu FX, Wang J, Wang DD, Jia XB, Tang DQ, and Chen B
- Subjects
- Animals, Biotransformation, Female, Male, Panax chemistry, Plant Extracts chemistry, Ginsenosides metabolism, Zebrafish metabolism
- Abstract
Ginsenosides F
4 and Rg6 (GF4 and GRg6 ), two main active components of steamed notoginseng or red ginseng, are dehydrated disaccharide saponins. In this work, biotransformation of ginsenosides F4 and Rg6 in zebrafish was investigated by qualitatively identifying their metabolites and then proposing their possible metabolic pathways. The prediction of possible metabolism of ginsenosides F4 and Rg6 using zebrafish model which can effectively simulate existing mammals model was early and quickly performed. Metabolites of ginsenosides F4 and Rg6 after exposing to zebrafish for 24 h were identified by Ultraperformance Liquid Chromatography/Quadrupole-Time-of-Flight Mass Spectrometry. A total of 8 and 6 metabolites of ginsenosides F4 and Rg6 were identified in zebrafish, respectively. Of these, 7 and 5, including M1, M3-M5, M7-M9 and N1 (N5), N2, N4 (N9), N7-N8 were reported for the first time as far as we know. The mechanisms of their biotransformation involved were further deduced to be desugarization, glucuronidation, sulfation, dehydroxylation, loss of C-17 and/or C-23 residue pathways. It was concluded that loss of rhamnose at position C-6 and glucuronidation at position C-3 in zebrafish were considered as the main physiologic and metabolic processes of ginsenosides F4 and ginsenosides Rg6 , respectively.- Published
- 2018
- Full Text
- View/download PDF
8. Qualitative and quantitative analysis of the major constituents in traditional Chinese medicine Danmu injection using LC-ESI-MS(n) and LC-DAD.
- Author
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Zhu FX, Wang JJ, Li XF, Sun E, and Jia XB
- Abstract
Background: Danmu injection, a traditional Chinese medicine (TCM) preparation made from Nauclea officinalis, has been commonly used for the treatment of cold, fever, swelling of throat in China. However, the chemical constituents in Danmu injection have not been clarified yet., Objective: a HPLC/DAD/ESI-MS(n) method was developed for qualitative and quantitative analysis of the components in Danmu injection., Materials and Methods: The chromatographic separation was performed on a Welch Material XB-C18 (4.6mm × 250mm, 5μm) using gradient elution with acetonitrile (A) and water containing 0.1% formic acid (B) as mobile phase at a flow rate of 1.0 ml/min., Results: Twenty-five compounds, including phenolic acid and phenol glycoside, iridous glycoside and glycoalkaloid were identified or tentatively deduced on the base of their retention behaviors, UV absorption, MS and MS(n) data with those elucidated references or literature. In addition, eleven compounds were simultaneously determined by HPLC-DAD, which was validated and successfully applied for determination of major components in Danmu injection., Conclusion: The results suggested that the established qualitative and quantitative method would be a powerful and reliable analytical tool for the characterization of multi-constituent in complex chemical system and quality control of Danmu injection.
- Published
- 2014
- Full Text
- View/download PDF
9. Preparation of icariside II-phospholipid complex and its absorption across Caco-2 cell monolayers.
- Author
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Jin X, Zhang ZH, Sun E, Tan XB, Zhu FX, Li SL, and Jia XB
- Subjects
- Absorption, Biological Transport, Active, Caco-2 Cells, Calorimetry, Differential Scanning, Cell Membrane chemistry, Cell Membrane metabolism, Chromatography, High Pressure Liquid, Data Interpretation, Statistical, Electric Impedance, Humans, Spectrophotometry, Infrared, Spectrophotometry, Ultraviolet, Flavonoids chemistry, Phospholipids chemistry
- Abstract
In the present study, an icariside II-phospholipid complex was prepared, and its physicochemical properties including UV spectrum, IR spectrum, differential scanning calorimetry (DSC) were tested. Furthermore, the absorption of icariside II and icariside II-phospholipid complex was compared in a Caco-2 cell culture model. The results show that icariside II-phospholipid complex could significantly increase the A-B transport of icariside II (P < 0.01), with A-B Papp of (3.92 +/- 0.50) x 10(-6) cm/s compared to control (2.05 +/- 0.18) x 10(-6) cm/s (increased by 91%). Likewise, the B-A transport of icariside II was significantly enhanced (P < 0.01), with Papp of (15.3 +/- 0.72) x 10(-6) cm/s (control) to (22.4 +/- 1.4) x 10(-6) cm/s (46% increase). Efflux ratio of icariside II decreased by 23.5% after forming icariside II-phospholipid complex. The therefore, it could be concluded that phospholipid complexation can increase the intestinal absorption of icariside II.
- Published
- 2012
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