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Background: In Gabon, studies on the characterization of extended-spectrum beta-lactamase-producing Escherichia coli in young children with diarrhoea are almost nonexistent. The objective was to evaluate the prevalence of antibiotic resistance to extended-spectrum beta-lactamase-producing Escherichia coli in children at public hospitals in Franceville, Gabon. Methods: Seventy diarrhoea faecal samples were collected from children aged 0–5 years. The culture and isolation of colonies were carried out on MacConkey agar. The colonies were identified using VITEK 2. The determination of the extended-spectrum beta-lactamase's profiles was accomplished using the double disk method. The identification of phylogroups and pathotypes was performed by PCR. Identification of the ESBL genes was performed by sequencing. Results: A total of 26 strains of Escherichia coli (33.0%) were identified from 78 bacterial isolates. Twenty (77.0%) Escherichia coli strains carried extended-spectrum beta-lactamases blaCTX-M-15 and 5.0% carried blaSHV-12 subtypes. Phylogroup D (62.0%) was predominant, followed by B1 (12.0%), B2 (8.0%) and E (4.0%). The bacterial pathogens causing diarrhoea were enterohemorrhagic E. coli (12.0%), typical enteropathogenic Escherichia coli (8.0%), atypical enteropathogenic Escherichia coli (4.0%), Enteroaggregative Escherichia coli (4.0%) and enteroinvasive E. coli (4.0%). Conclusions: This study showed a high prevalence of extended-spectrum beta-lactamase, Escherichia coli of phylogroup D and pathotype enterohemorrhagic Escherichia coli in children under 5 years old in public hospitals in Franceville, most probably due to the misuse or inappropriate consumption of beta-lactams. [ABSTRACT FROM AUTHOR]

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Background: Malaria remains a threat to the health of the population of Gabon based on the high hospitalisations and deaths caused by this disease. This study aimed at determining the prevalence and associated risk factors of malaria in febrile patients visiting the Centre de Santé d’Okala (CSO). Methods: A six years study was conducted on febrile patients seeking for health services at the CSO from the year 2014 to 2019. Blood samples of 2388 patients were tested for antigens specific to Plasmodium falciparum using the Beacon® rapid diagnostic kit. Results: The overall prevalence of P. falciparum malaria was 29.857%. The year 2014 (35.553%) recorded the highest prevalence rate. Individuals from Okala (44.137%) community recorded the highest number of cases. Females (56.886%) had higher infection prevalence than their male (43.114%) counterparts. Children ≤ 10 years old (57.412%) had the highest prevalence rates than other age cohorts. Conclusions: The number of malaria cases reported in the CSO are currently increasing and community sensitisation on preventive measures against this disease is required in order to flatten the curve of positive cases. [ABSTRACT FROM AUTHOR]