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This paper outlines the practice of a novel digital method in animal geographies: etho-ethnographic citizen science. I describe a project using this participatory method with local residents in inner-city London, where we worked together to use camera traps to record video footage of red fox behaviour. The research sought to build an etho-ethnographic account of fox life by tethering data collection and interpretation to local knowledge. The paper focuses on the familial relations of one particular fox, a young male living on an allotment, who plotholders call Sonny. It begins by outlining how research objectives emerged through the process of collaborative research design with plotholders, premised on their own knowledge of fox personalities, and their storied accounts of individual foxes. It then considers how the practical planning of camera placement was directed through the plotholders own socioecological knowledge of the site. Lastly, it outlines how participants continual use of the traps, and their own analysis of footage, embeds digital data within vernacular understandings of Sonny's world. In doing so the paper outlines how etho-ethnographic citizen science can potentially amplify, affirm and digitise vernacular knowledges of urban fox ethologies and geographies. [ABSTRACT FROM AUTHOR]

In this co-written article creative practice researchers Christine Rogers (CR) and Liz Burke (LB) explicate their reasons for, processes, and outcomes of filming animals. Christine filmed the wild foxes who inhabited her Belfast garden. She set up a wildlife camera, gaining an up-close encounter with these shy strangers, and hoping through this, to create a sense of belonging for herself, a stranger to Northern Ireland. Liz filmed her two whippets, Agnes and then Zelda, to uncover the mysterious connections in the deep relationships between herself and her dogs. Both understand the ethical dilemmas of such filming and seek to make sense of this. Both adhere to Donna Haraway's call to enter into a complex dance with the other. Christine cedes control of the shooting and with her decolonising ethos she explores the tricky idea of reciprocity. Liz chooses to cede control in the editing as she works with a complexity-generating programme Korsakow. For Christine, belonging is created, and it becomes clear that she must remain a stranger to the foxes, no matter her desire for an intimate connection. Her desire for reciprocity also remains unmet. For Liz, belonging is not so much created as revealed by the strength of the connection between herself and her dogs. [ABSTRACT FROM AUTHOR]

Carnivores play key roles in maintaining ecosystem structure and function as well as ecological processes. Understanding how sympatric species coexist in natural ecosystems is a central research topic in community ecology and biodiversity conservation. In this study, we explored intra- and interspecific niche partitioning along spatial, temporal, and dietary niche partitioning between apex carnivores (wolf Canis lupus, snow leopard Panthera uncia, Eurasian lynx Lynx lynx) and mesocarnivores (Pallas’s cat Otocolobus manul, red fox Vulpes vulpes, Tibetan fox Vulpes ferrilata) in Qilian Mountain National Park, China, using camera trapping data and DNA metabarcoding sequencing data. Our study showed that apex carnivore species had more overlap temporally (coefficients of interspecific overlap ranging from 0.661 to 0.900) or trophically (Pianka’s index ranging from 0.458 to 0.892), mesocarnivore species had high dietary overlap with each other (Pianka’s index ranging from 0.945 to 0.997), and apex carnivore and mesocarnivore species had high temporal overlap (coefficients of interspecific overlap ranging from 0.497 to 0.855). Large dietary overlap was observed between wolf and snow leopard (Pianka’s index = 0.892) and Pallas’s cat and Tibetan fox (Pianka’s index = 0.997), suggesting the potential for increased resource competition for these species pairs. We concluded that spatial niche partitioning is likely to key driver in facilitating the coexistence of apex carnivore species, while spatial and temporal niche partitioning likely facilitate the coexistence of mesocarnivore species, and spatial and dietary niche partitioning facilitate the coexistence between apex and mesocarnivore species. Our findings consider partitioning across temporal, spatial, and dietary dimensions while examining diverse coexistence patterns of carnivore species in Qilian Mountain National Park, China. These findings will contribute substantially to current understanding of carnivore guilds and effective conservation management in fragile alpine ecosystems. [ABSTRACT FROM AUTHOR]

Canine adenovirus (CAdV) had a high prevalence in fox populations and induced fox encephalitis. No ELISA kits specifically for CAdV-1 antigen had been commercialized for foxes in China. It is crucial to develop a rapid and accurate ELISA method for detecting of CAdV-1. The monoclonal antibodies (mAbs, IgG1A) and HRP-labeled polyclonal antibodies (pAbs) were used to establish the ELISA method in this experiment. The results showed that the optimal concentration and coating time for the mAbs (IgG1A) were 2.15 µg/mL and overnight at 4°C, respectively. The dilution ratio of the HRP-labeled pAbs was 1:2000. Five percent skimmed milk was selected as the blocking agent. The optimal incubation times for blocking, CAdV-1, and HRP-labeled pAbs were all 1 h. The cut-off value for negative rectal swab was determined to be 0.366 ± 0.032. The maximum dilution ratio was 100 TCID50/mL. The ELISA method was positive to CAdV-1, and that was negative to CAdV-2, Canine Parvovirus (CPV) and Canine Distempervirus (CDV). The ELISA method showed good repeatability, sensitivity, and specificity. Compared with RT-PCR, the sensitivity, specificity, and coincidence rates of the ELISA method were 93.75, 90.9, and 92.86%, respectively. These results indicate that the established ELISA method can be used for the large-scale screening and epidemiology surveillance of CAdV-1 in foxes. [ABSTRACT FROM AUTHOR]

Adaptive spiral flight and multi-strategy fusion are the foundations of a new FOX optimization algorithm that aims to address the drawbacks of the original method, including weak starting individual ergodicity, low diversity, and an easy way to slip into local optimum. In order to enhance the population, inertial weight is added along with Levy flight and variable spiral strategy once the population is initialized using a tent chaotic map. To begin the process of implementing the method, the fox population position is initialized using the created Tent chaotic map in order to provide more ergodic and varied individual beginning locations. To improve the quality of the solution, the inertial weight is added in the second place. The fox random walk mode is then updated using a variable spiral position updating approach. Subsequently, the algorithm's global and local searches are balanced, and the Levy flying method and greedy approach are incorporated to update the fox location. The enhanced FOX optimization technique is then thoroughly contrasted with various swarm intelligence algorithms using engineering application optimization issues and the CEC2017 benchmark test functions. According to the simulation findings, there have been notable advancements in the convergence speed, accuracy, and stability, as well as the jumping out of the local optimum, of the upgraded FOX optimization algorithm. [ABSTRACT FROM AUTHOR]

Resource distribution, habitat structure, and predators greatly influence spatial and temporal landscape use by prey species. The "risky places" hypothesis establishes prey will proactively respond to predators' presence based on habitat cues, whereas the "risky times" hypothesis predicts prey will reactively respond by increasing vigilance in the presence of predators regardless of habitat cues. We fit a multiscale, Bayesian species interaction occupancy model with detection/non‐detection data to evaluate black‐tailed jackrabbit (Lepus californicus) and eastern cottontail rabbit (Sylvilagus floridanus) habitat use in the presence and absence of coyotes (Canis latrans), American badgers (Taxidea taxus), and swift foxes (Vulpes velox). We also evaluated how species‐specific predator presence modified temporal activity patterns of prey. Jackrabbits decreased habitat use in areas with greater forage and opted to use areas with greater visibility when coyotes or swift foxes were present. However, cottontails used habitat in open areas with greater visibility when American badgers were present and all other predators absent, suggesting dissimilar habitat‐use patterns dictated by predator‐specific risks. Both lagomorph species are nocturnal with segregated peaks of activity compared with predators, suggesting fine‐scale temporal use partitioning. Our results provide insights into predator–prey dynamics across heterogenous landscapes in a multi‐predator system. [ABSTRACT FROM AUTHOR]

Purpose: Crenosoma vulpis (Dujardin,1845) is a lungworm which has spread worldwide in canines and is associated with upper respiratory infections. In a majority of cases, the infections are accompanied with chronic cough. Diagnosis of lungworms is often underdiagnosed and can be misinterpreted as other respiratory diseases. Methods: The Small Animal Clinic of the University Veterinary Hospital admitted an 11-month-old dog presented with persistent cough associated with difficulty in breathing and even asphyxia. Based on clinical symptoms, the patient underwent radiological and bronchoscopic examination. Bronchoscopy revealed the presence of lungworms obturating the branches of the tracheobronchial tree. Larvae were collected by bronchoscopic lavage and subjected to parasitological and molecular examination. Results: Microscopic detection and morphological identification of the worms removed during the bronchoscopy confirmed the presence of female adult worms. The subsequent molecular characterisation of the mitochondrial (cytochrome c oxidase subunit I gene (cox1) and 12S ribosomal DNA (rDNA)), nuclear (18S rDNA) genes, as well as the analysis of the second internal transcribed spacer (ITS-2) region of the ribosomal DNA, confirmed the Crenosoma vulpis species. Faecal samples were processed using the Baermann method, which confirmed the presence of the larval stage 1 of C. vulpis. The therapy with fenbendazole at a dose of 50 mg/kg of live weight once daily for the period of 7 days was initiated for the patient. Conclusion: This paper presents the first molecularly confirmed clinical case of a Crenosoma vulpis infection in an 11-month-old female dog of the Miniature Schnauzer breed in Slovakia. [ABSTRACT FROM AUTHOR]

Identifying spatial clusters of rabies in animals aids policymakers in allocating resources for rabies prevention and control. This study aimed to investigate spatial patterns and hotspots of rabies in different animal species at the county level in New Jersey. Data on animal rabies cases from January 1989 to December 2023 were obtained from the New Jersey Department of Health and aggregated by county. Global Moran's index (I) statistics were computed for each species to detect global spatial clustering (GeoDa version 1.22). Local Moran's indicators of spatial association (LISA) were computed to identify local clusters of rabies. The results from the LISA analysis were mapped using ArcGIS Pro to pinpoint cluster locations. A total of 9637 rabies cases were analyzed among raccoons (n = 6308), skunks (n = 1225), bats (n = 1072), cats (n = 597), foxes (n = 225), and groundhogs (n = 210). A global Moran's test indicated significant global spatial clustering in raccoons (I = 0.32, p = 0.012), foxes (I = 0.29, p = 0.011), and groundhogs (I = 0.37, p = 0.005). The LISA results revealed significant spatial clustering of rabies in raccoons and foxes in southeastern New Jersey and in groundhogs in northern New Jersey. These findings could guide the development of targeted oral rabies vaccination programs in high-risk New Jersey counties, reducing rabies exposure among domestic animals and humans. [ABSTRACT FROM AUTHOR]

We tested if the personality of captive-raised western ringtail possums (Pseudocheirus occidentalis), and the impact of other variables would influence their survival after release using radiotelemetry. We hypothesised a greater survival for individuals: (i) bold; (ii) habituated in advance to food collected from the release area; (iii) juveniles instead of adults, because more easily tolerated by wild individuals, and (iv) released in new dreys (nests) as they would not have the strong odour of old dreys and would be less attractive to foxes. After 3 months of radio tracking, out of 143 possums released, 79 died: 51 (64.6%) were preyed upon by European red foxes (Vulpes vulpes). Bold or female individuals had higher survival rates than shy or male individuals (survival rate bold: 53%, shy: 41%, p = 0.046, hazard ratio = 0.352, 95% CI HR [0.126, 0.979]; survival rate females: 44%, males: 35%, p = 0.007, hazard ratio = 2.811, 95% CI HR [1.322, 5.976]). Shooting was a more effective fox control strategy to improve survival compared to baiting (p = 0.019, hazard ratio = 0.167, 95% CI HR [0.038, 0.742]). Our results demonstrate that the control of introduced predators is critical for the success of reintroductions of this critically endangered species. [ABSTRACT FROM AUTHOR]

Canine distemper (CD) is a virulent disease caused by the canine distemper virus (CDV) in canines and mustelidaes with high mortality. The incidence of CDV is worldwide distribution and it has caused huge economic losses to multiple industries around the world. There are many studies investigating the prevalence of CD infection, but no comprehensive analysis of CDV infection in minks, foxes and raccoon dogs worldwide has therefore been carried out. The aim of this meta is to provide a comprehensive assessment of the prevalence of CDV infection in minks, foxes and raccoon dogs dogs through a meta-analysis of articles published from around the world. Data from 8,582 small carnivores in 12 countries were used to calculate the combined prevalence of CD. A total of 22.6% (1,937/8,582) of minks, foxes and raccoon dogs tested positive for CD. The prevalence was higher in Asia (13.8, 95% CI: 22.2-45.6), especially in South Korea (65.8, 95% CI: 83.3-95.8). Our study found that the incidence of CD was also associated with geographic climate, population size, health status, and breeding patterns. CD is more commonly transmitted in minks, foxes and raccoon dogs. However, the concentrated breeding as an economic animal has led to an increase in the prevalence rate. The difference analysis study recommended that countries develop appropriate preventive and control measures based on the prevalence in the minks, foxes, and raccoon dogs industries, and that reducing stocking density is important to reduce the incidence of CDV. In addition, CDV is more common in winter, so vaccination in winter should be strengthened and expanded to reduce the incidence of CD in minks, foxes and raccoon dogs. [ABSTRACT FROM AUTHOR]

Sympatric species reduce competitive interactions by segregating their ecological niche as a strategy that promotes coexistence. The main dimensions in which niche differentiation occurs are spatial, trophic, and temporal axes. Thus, describing the spatiotemporal patterns of sympatric species help us better grasp how species coexist. In Mexico, coyotes (Canis latrans) and gray foxes (Urocyon cinereoargenteus) are sympatric and widely distributed, and information about their temporal and spatiotemporal interactions is still needed to explain their coexistence throughout their distribution. The evaluation of the ecological niche in temperate forests where both species coexist has shown high spatial and trophic overlap. Therefore, more pronounced segregation must occur in other niche dimensions, such as temporal and spatiotemporal axes, to maintain the coexistence between coyotes and gray foxes. As an approach to exploring the coexistence between these species in temperate forests, we pose the following question: Is there temporal or spatiotemporal segregation between sympatric coyotes and gray foxes? We hypothesized that both species maintain coexistence due to differences in their daily activity patterns (temporal segregation) and the presence of avoidance behaviors at the spatiotemporal level (spatiotemporal segregation), thereby reducing agonistic interactions. We aimed to evaluate indicators of niche segregation through two approaches: (i) temporal niche partitioning, measuring the overlap of the daily activity patterns and activity levels; and (ii) spatiotemporal niche partitioning, through temporal co-occurrence analysis and time to encounter analysis. In 2018, we used a camera-trap array (n = 43) to evaluate spatiotemporal interactions of coyotes and gray foxes in temperate forests of northern Mexico. We found that coyotes exhibited a diurnal daily activity pattern, whereas gray foxes were nocturnal, showing low overlap between daily activity patterns. Temporal co-occurrence and time to encounter analysis showed that coyotes and gray foxes occurred independently of each other. Therefore, we demonstrated that the spatiotemporal interactions between both species is a result of random species associations. In conclusion, here we provide evidence that ecological segregation occurred in the temporal niche and not in the spatiotemporal niche of the species. Thus, the temporal niche segregation represents one of the ecological strategies that facilitates the coexistence between coyotes and gray foxes and therefore promotes their sympatry in the temperate forests of northern Mexico. [ABSTRACT FROM AUTHOR]

Large carnivores are being globally reintroduced with the goal of restoring ecological interactions. However, the extent that competitive interactions are restored within communities is often unclear. In a before–after study within Isle Royale National Park (in the US state of Michigan), we quantified the spatial, behavioral, trophic, and demographic effects of the reintroduction of a large carnivore (gray wolf; Canis lupus) on meso‐carnivores (red fox; Vulpes vulpes) and small carnivores (American marten; Martes americana). The wolf reintroduction produced a phase‐dependent pulse perturbation: wolves constrained the distribution of foxes, thereby benefiting martens, yet foxes altered their behavior, notably using human‐provided resource subsidies (campsites and food) more frequently, which buffered demographic consequences. Once wolf packs coalesced, all observed changes subsided, and competitive interactions returned to their pre‐wolf values. Our results show that some predicted—and often desired—consequences of large carnivore reintroductions may not be permanent due to the transitory dynamics of social carnivores and the presence of humans, even within a "pristine wilderness". [ABSTRACT FROM AUTHOR]

Foxes are susceptible to SARS-CoV-2 in laboratory settings, and there have also been reports of natural infections of both SARS-CoV and SARS-CoV-2 in foxes. In this study, we assessed the binding capacities of fox ACE2 to important sarbecoviruses, including SARS-CoV, SARS-CoV-2, and animal-origin SARS-CoV-2 related viruses. Our findings demonstrated that fox ACE2 exhibits broad binding capabilities to receptor-binding domains (RBDs) of sarbecoviruses. We further determined the cryo-EM structures of fox ACE2 complexed with RBDs of SARS-CoV, SARS-CoV-2 prototype (PT), and Omicron BF.7. Through structural analysis, we identified that the K417 mutation can weaken the ability of SARS-CoV-2 sub-variants to bind to fox ACE2, thereby reducing the susceptibility of foxes to SARS-CoV-2 sub-variants. In addition, the Y498 residue in the SARS-CoV RBD plays a crucial role in forming a vital cation-π interaction with K353 in the fox ACE2 receptor. This interaction is the primary determinant for the higher affinity of the SARS-CoV RBD compared to that of the SARS-CoV-2 PT RBD. These results indicate that foxes serve as potential hosts for numerous sarbecoviruses, highlighting the critical importance of surveillance efforts. [ABSTRACT FROM AUTHOR]

This study aims to understand urban animal welfare policy development and implementation for four species in public spaces: cats (Felis catus), dogs (Canis familiaris), pigeons (Columbia livia domestica), and foxes (Vulpes vulpes). Our exploratory research offers an overview of the perspectives of all involved municipal and police officers and their challenges in a metropolitan urban context, the Brussels Capital Region in Belgium. We conducted semi-structured interviews with 35 participants from 19 municipalities and six police zones of the region. Additionally, we organized two focus groups consisting of representatives from municipalities, police zones, and nongovernmental organizations. Afterward, we analyzed the data thematically, leading to the identification of six policy and six practice recommendations. These recommendations can help advance the notion of urban animal welfare for the four species from a multispecies perspective. [ABSTRACT FROM AUTHOR]

Prey-predator interactions across time and space strongly influence many animal communities. This study unveils the first insights into the ecology of the Eurasian red squirrel (Sciurus vulgaris) in Mongolia, and specifically in Bogd Khan Mountain Strictly Protected Area. We used by-catch data obtained from camera traps to analyze activity patterns and potential interactions with mammalian predators. Red squirrels exhibited primarily diurnal activity with occasional nocturnal forays, while potential predators like foxes (Vulpes vulpes, Vulpes corsac) and martens (Martes foina, Martes zibellina) displayed cathemeral and nocturnal patterns, respectively. The diurnal Pallas’s cat (Otocolobus manul) showed the greatest activity overlap with squirrels. Suggesting predator avoidance behavior potentially mediated by olfactory cues, squirrel activity was negatively impacted by marten presence, which was in turn potentially affected by human disturbance. We highlight the need for further investigations, particularly regarding the dietary composition of Pallas’s cats in forested habitats and the broader ecological implications of human disturbance on predator–prey dynamics within this crucial conservation area. [ABSTRACT FROM AUTHOR]

Computed tomography (CT) is a non-invasive, three-dimensional imaging tool used in medical imaging, forensic science, industry and engineering, anthropology, and archaeology. The current study used high-resolution medical CT scanning of 431 animal skulls, including 399 dog skulls from 152 breeds, 14 cat skulls from 9 breeds, 14 skulls from 8 wild canid species (gray wolf, golden jackal, coyote, maned wolf, bush dog, red fox, Fennec fox, bat-eared fox), and 4 skulls from 4 wild felid species (wildcat, leopard, serval, caracal). This comprehensive and unique collection of CT image series of skulls can provide a solid foundation not only for comparative anatomical and evolutionary studies but also for the advancement of veterinary education, virtual surgery planning, and the facilitation of training in sophisticated machine learning methodologies. [ABSTRACT FROM AUTHOR]

The hoary fox (Lycalopex vetulus) is the only species of the Canidae (Mammalia: Carnivora) endemic to Brazil, and so far has been the target of few genetic studies. Using microsatellites and mtDNA markers, we investigated its present genetic diversity and population structure. We also tested the hypothesis that this species currently hybridizes with the pampas fox (L. gymnocercus), as suggested by previous mtDNA data from two individuals. We collected tissue and blood samples from animals representing most of the two species' distributions in Brazil (n  = 87), including their recently discovered geographic contact zone in São Paulo state. We observed that the hoary fox exhibits high levels of genetic diversity and low levels of population structure. We identified six individuals from São Paulo state with clear evidence of hybridization based on introgressed pampas fox mitochondrial DNA and/or admixed microsatellite genotypes (three individuals bore both types of evidence). These results demonstrate the existence of admixed individuals between hoary and pampas foxes in southeastern Brazil, representing the first identified case of interspecies admixture between native South American canids. We discuss our findings in the context of the evolutionary history of these foxes and address potential conservation implications of this interspecies hybridization process. [ABSTRACT FROM AUTHOR]

The first record of captive-bred red foxes (Vulpes vulpes) dates to 1896 when a breeding enterprise emerged in the provinces of Atlantic Canada. Because its domestication happened during recent history, the red fox offers a unique opportunity to examine the genetic diversity of an emerging domesticated species in the context of documented historical and economic influences. In particular, the historical record suggests that North American and Eurasian farm-bred populations likely experienced different demographic trajectories. Here, we focus on the likely impacts of founder effects and genetic drift given historical trends in fox farming on North American and Eurasian farms. A total of 15 mitochondrial haplotypes were identified in 369 foxes from 10 farm populations that we genotyped (n  = 161) or that were previously published. All haplotypes are endemic to North America. Although most haplotypes were consistent with eastern Canadian ancestry, a small number of foxes carried haplotypes typically found in Alaska and other regions of western North America. The presence of these haplotypes supports historical reports of wild foxes outside of Atlantic Canada being introduced into the breeding stock. These putative Alaskan and Western haplotypes were more frequently identified in Eurasian farms compared to North American farms, consistent with historical documentation suggesting that Eurasian economic and breeding practices were likely to maintain low-frequency haplotypes more effectively than in North America. Contextualizing inter- vs. intra-farm genetic diversity alongside the historical record is critical to understanding the origins of this emerging domesticate and the relationships between wild and farm-bred fox populations. [ABSTRACT FROM AUTHOR]

The mammalian vomeronasal system enables the perception of chemical signals crucial for social communication via the receptor families V1R and V2R. These receptors are linked with the G-protein subunits, Gαi2 and Gαo, respectively. Exploring the evolutionary pathways of V1Rs and V2Rs across mammalian species remains a significant challenge, particularly when comparing genomic data with emerging immunohistochemical evidence. Recent studies have revealed the expression of Gαo in the vomeronasal neuroepithelium of wild canids, including wolves and foxes, contradicting predictions based on current genomic annotations. Our study provides detailed immunohistochemical evidence, mapping the expression of V2R receptors in the vomeronasal sensory epithelium, focusing particularly on wild canids, specifically wolves and foxes. An additional objective involves contrasting these findings with those from domestic species like dogs to highlight the evolutionary impacts of domestication on sensory systems. The employment of a specific antibody raised against the mouse V2R2, a member of the C-family of vomeronasal receptors, V2Rs, has confirmed the presence of V2R2-immunoreactivity (V2R2-ir) in the fox and wolf, but it has revealed the lack of expression in the dog. This may reflect the impact of domestication on the regression of the VNS in this species, in contrast to their wild counterparts, and it underscores the effects of artificial selection on sensory functions. Thus, these findings suggest a more refined chemical detection capability in wild species. [ABSTRACT FROM AUTHOR]

In Australia, most threatened species translocations conducted into areas where feral predators are present fail to establish viable, self‐sustaining populations despite intensive predator control. These translocations are occurring amidst a lack of understanding regarding the conditions required for native species to survive, including predator densities. This study investigated whether population trends of in situ common species could be used as bioindicators to determine the effectiveness of predator management for threatened species protection. We compared changes in capture rates of four small mammals and four reptile species inside and outside a predator‐proof reserve for 11 years after cat and fox removal to identify which species responded to cat and fox eradication at various time scales. We only used sites inside the reserve where threatened species had not yet been reintroduced to isolate the effects of cat and fox removal. The effectiveness of these bioindicators was then tested at an unfenced reserve where predators were controlled using baiting, trapping, and shooting. There was a significant increase in the abundance of native rodents (spinifex hopping mouse and Bolam's mouse) inside the fenced reserve compared to outside, however, these differences were not detected in the unfenced reserve possibly due to inadequate predator control or insufficient time for in situ species to recover with sustained predator control. Captures of the introduced house mouse were higher at sites inside the fenced reserve in some years, but the difference was not consistent. Native dunnarts and all four reptile species did not respond consistently over the 11‐year time frame at either reserve. Native rodent abundance was the best indicator of effective feral predator control and may provide a useful bioindicator for threatened species management, such as reintroductions. [ABSTRACT FROM AUTHOR]

We performed a retrospective study of all case submissions for the rabies virus (RABV) direct fluorescent antibody test (DFAT) requested of the Tifton Veterinary Diagnostic and Investigational Laboratory (Tifton, GA, USA) between July 2010 and June 2021. Submitted were 792 samples from 23 animal species from 89 counties in Georgia, and 4 neighboring counties in Florida, 1 in South Carolina, and 1 in Alabama. In 13 (1.6%) cases, the DFAT result was inconclusive; 779 (98.4%) cases had a conclusive (positive or negative) test result. Of these 779 cases, 79 (10.1%) tested positive across 10 species. The remaining 700 (89.9%) cases were negative. The main reason for submission for RABV testing was human exposure to a potentially rabid animal in 414 (52.3%) cases. Among the 79 positive cases, 74 (93.7%) involved wildlife; raccoons (51 cases; 68.9%) were the primary host confirmed with RABV infection, followed by skunk and fox (8 cases each; 10.8%), bobcat (5 cases; 6.8%), and bats (2 cases; 2.7%). Only 5 domestic animals (6.3% of the positive cases) tested positive during our study period; one from each of the bovine, canine, caprine, equine, and feline species. Hence, the sylvatic cycle plays the predominant role in circulating RABV infection in our study area. [ABSTRACT FROM AUTHOR]

Due to the increasing cohabitation of humans with infected animals, such as pets or wildlife, human infection with helminthic parasites has become an important emerging health concern. This study aimed to investigate the prevalence of helminthic parasite infections in canines in the Mughan Plain area of Ardabil Province, Iran. A total of 87 samples were collected from different canines, including stray dogs, foxes, and jackals, in the Mughan Plain area of Ardabil Province, Iran. The organs of the animals were sectioned and examined macroscopically for helminth parasitic infections. Isolated helminth species were morphologically identified using a valid identification key. The results showed that that Mesocestoides sp. was the most prevalent helminth species, with a frequency of 30 in canids (34.5%), including 8 red foxes (Vulpes vulpes, 53.33%), 12 jackals (Canis aureus, 48%), and 10 stray dogs (Canis familiaris, 21.3%). In addition, a high frequency of Toxascaris leonina was observed in 13 canines (14.9%), including 2 foxes (13%), 4 jackals (16%), and 7 wild dogs (14.9%). The prevalence of Echinococcus granulosus was reported in 9 canines (10.3%), including 1 jackal (4%) and 8 wild dogs (17.02%). The results of this study indicate that Mesocestoides sp. is the most common helminth species in canids. Our findings indicate that canines in Iran serve as both intermediate and final definitive hosts for several harmful parasites, posing a risk to humans and animals health. Further research and interventions are needed to better understand the transmission dynamics and to develop effective strategies for helminth control in canids. [ABSTRACT FROM AUTHOR]

Coyotes (Canis latrans) are believed to contribute to declining kit fox (Vulpes macrotis) numbers in the Great Basin desert through intraguild predation. Intraguild prey have been shown to exhibit adaptive compromise, whereby an animal increases selection for risky, but food-rich areas during times of food stress (i.e. winter). We evaluated the habitat selection of kit foxes in the Great Basin desert to elucidate if they demonstrated adaptive compromise as a method of coexisting with coyotes. We created 2nd order resource selection functions to analyze kit fox habitat selection associated with coyote relative probability of use (RPU), prey abundance, and type of soil substrate. In the summer, we found that kit fox selection for areas of relatively more abundant prey was not significant, and there was a small positive selection for coyote RPU. In the winter, we found a positive relationship between kit fox selection and prey abundance as well as a stronger selection for coyote RPU. These findings do follow the pattern of adaptive compromise. We also found kit foxes selected for silty and sandy soils, which are conducive to den construction, as they use dens seasonally for breeding but also year-round for multiple uses, including refugia from predators and extreme heat. Soil substrate appeared to be an important factor impacting kit fox habitat selection. [ABSTRACT FROM AUTHOR]

Pampas and crab-eating foxes are medium-sized canids living in sympatry in the middle east of South America. Studies on the diet composition of these species provide a deep understanding of their ecological roles in the ecosystem structure and regulation. Using the metabarcoding technique, we analyzed the diet of both fox species in order to identify the vertebrate taxa included as food items. A fragment of the 12S ribosomal gene of the mtDNA was amplified using DNA extracted from 27 scat samples collected in south-central Uruguay during cold (June 2015) and warm (January April 2016) seasons. A fox DNA blocking primer was designed to minimize the host amplicon products, and pooled samples were sequenced through paired-end reads (100 bp library) on a MiSeq Illumina Platform. The generated sequences were compared to a reference database built with sequences available in GenBank. In concordance with previous studies using traditional methods, we found that the most common food taxon were rodents. Qualitative differences in diet composition between both fox species were identified. Armadillo species were only found in pampas fox diet, while a greater variety of amphibians and birds were detected in crab-eating fox feces. Additionally, an innovative approach to differentiate between real and artifact sequences was employed. This method was based on comparing mutations at conserved and non-conserved positions within the secondary structure of the 12S rRNA, combined with network sequence reconstruction. Our results demonstrate the efficacy of the methodology in detecting the food species present in both fox diets, enabling the evaluation of intraspecific diversity among these species and facilitating the discarding of sequencing errors. This makes the methodology applicable to a wide range of studies. [ABSTRACT FROM AUTHOR]

[Display omitted] • Individual foxes spreading Echinococcus multilocularis were tracked using copro-sampling. • Over 4 years, 45 foxes were identified from a collection of 124 genotyped fox faecal samples. • One-third of individual E. multilocularis- infected foxes deposited two-thirds of the positive faecal samples. • Three fox genetic clusters were highlighted, with one clustering two-thirds of the positive faeces. • Female foxes had a higher average number of multiple-stool deposits compared with males. Key parasite transmission parameters are difficult to obtain from elusive wild animals. For Echinococcus multilocularis , the causative agent of alveolar echinococcosis (AE), the red fox is responsible for most of the environmental contamination in Europe. The identification of individual spreaders of E. multilocularis environmental contamination is crucial to improving our understanding of the ecology of parasite transmission in areas of high endemicity and optimising the effectiveness of prevention and control measures in the field. Genetic faecal sampling appears to be a feasible method to gain information about the faecal deposition of individual animals. We conducted a 4 year faecal sampling study in a village that is highly endemic for E. multilocularis, to assess the feasibility of individual identification and sexing of foxes to describe individual infection patterns. Individual fox identification from faecal samples was performed by obtaining reliable genotypes from 14 microsatellites and one sex locus, coupled with the detection of E. multilocularis DNA, first using captive foxes and then by environmental sampling. From a collection of 386 fox stools collected between 2017 and 2020, tested for the presence of E. multilocularis DNA, 180 were selected and 124 samples were successfully genotyped (68.9%). In total, 45 unique individual foxes were identified and 26 associated with at least one sample which tested positive for E. multilocularis (Em (+)). Estimation of the population size showed the fox population to be between 29 and 34 individuals for a given year and 67 individuals over 4 years. One-third of infected individuals (9/26 Em (+) foxes) deposited 2/3 of the faeces which tested positive for E. multilocularis (36/60 Em (+) stools). Genetic investigation showed a significantly higher average number of multiple stools for females than males, suggesting that the two sexes potentially defecated unequally in the studied area. Three partially overlapping clusters of fox faeces were found, with one cluster concentrating 2/3 of the total E. multilocularis -positive faeces. Based on these findings, we estimated that 12.5 million E. multilocularis eggs were produced during the study period, emphasizing the high contamination level of the environment and the risk of exposure faced by the parasite hosts. [ABSTRACT FROM AUTHOR]

In this study, we investigated faecal specimens from legally hunted and road‐killed red foxes, raccoons, raccoon dogs, badgers and martens in Germany for parasites and selected zoonotic bacteria. We found that Baylisascaris procyonis, a zoonotic parasite of raccoons, had spread to northeastern Germany, an area previously presumed to be free of this parasite. We detected various pathogenic bacterial species from the genera Listeria, Clostridium (including baratii), Yersinia and Salmonella, which were analysed using whole‐genome sequencing. One isolate of Yersinia enterocolitica contained a virulence plasmid. The Salmonella Cholerasuis isolate encoded an aminoglycoside resistance gene and a parC point mutation, conferring resistance to ciprofloxacin. We also found tetracycline resistance genes in Paeniclostridium sordellii and Clostridium baratii. Phylogenetic analyses revealed that the isolates were polyclonal, indicating the absence of specific wildlife‐adapted clones. Predators, which scavenge from various sources including human settlements, acquire and spread zoonotic pathogens. Therefore, their role should not be overlooked in the One Health context. [ABSTRACT FROM AUTHOR]

Simple Summary: Control methods are applied worldwide to reduce predation on livestock by European red foxes (Vulpes vulpes). Lethal methods are intended to kill foxes and can inflict suffering. Non-lethal methods can also inflict suffering and can unintentionally lead to death. Moral debate about the animal welfare and ethical consequences of both methods is lacking. The aim of this study was to investigate the animal welfare consequences, the level of humaneness and the ethical and moral implications of the global use of fox control methods. Tom Regan's animal rights view and Peter Singer's utilitarian view were each considered. According to Regan, lethal or potentially harmful non-lethal methods ought not to be used because one should not interfere with wild animals and because the use of such methods violates the right of foxes not to be harmed or killed. According to Singer, if and only if the use of fox control methods can prevent suffering and death in livestock in a manner and with a magnitude that outweighs comparable suffering and death in foxes is one morally obligated to use them. It is clear that lethal fox control methods and some non-lethal methods are inhumane. Control methods are applied worldwide to reduce predation on livestock by European red foxes (Vulpes vulpes). Lethal methods can inflict suffering; however, moral debate about their use is lacking. Non-lethal methods can also inflict suffering and can unintentionally lead to death, and yet both the welfare consequences and ethical perspectives regarding their use are rarely discussed. The aim of this study was to investigate the animal welfare consequences, the level of humaneness, the ethical considerations and the moral implications of the global use of fox control methods according to Tom Regan's animal rights view and Peter Singer's utilitarian view. According to Regan, foxes ought not to be controlled by either lethal or potentially harmful non-lethal methods because this violates the right of foxes not to be harmed or killed. According to Singer, if an action maximises happiness or the satisfaction of preferences over unhappiness or suffering, then the action is justified. Therefore, if and only if the use of fox control methods can prevent suffering and death in livestock in a manner that outweighs comparable suffering and death in foxes is one morally obligated to use them. It is clear that lethal fox control methods and some non-lethal methods are inhumane. [ABSTRACT FROM AUTHOR]

Human presence and activities have profoundly altered animals' habitats, exposing them to greater risks but also providing new opportunities and resources. The animals' capacity to effectively navigate and strike a balance between risks and benefits is crucial for their survival in the Anthropocene era. Red foxes (Vulpes vulpes), adept urban dwellers, exhibit behavioral plasticity in human-altered environments. We investigated variations in detection frequency on trail cameras and the behavioral responses (explorative, bold, and fearful) of wild red foxes living along an urbanization gradient when exposed to a metal bin initially presented clean and then filled with anthropogenic food. All fox populations displayed an increased interest and similar explorative behavioral responses toward the anthropogenic food source, irrespective of the urbanization gradient. Despite no impact on explorative behaviors, foxes in more urbanized areas initially showed heightened fear toward the empty bin, indicating increased apprehension toward novel objects. However, this fear diminished over time, and in the presence of food, urban foxes displayed slightly reduced fear compared with their less urban counterparts. Our results highlight foxes' potential for adaptability to human landscapes, additionally underscoring the nuanced interplay of fear and explorative behavioral response of populations living along the urbanization gradient. [ABSTRACT FROM AUTHOR]

Scavengers benefit from carrion and simultaneously provide essential ecosystem services. To assess benefits and risks that carrion might bring, it is crucial to understand ecosystem-specific scavenger communities. Carrion research has mostly focussed on ungulate carcasses and has rarely explored the effects of carnivore carcasses, which can be critical to understanding disease transmission pathways. Therefore, using red fox, roe deer and red deer carcasses, we investigated factors that affect facultative vertebrate scavenger assemblages visiting carnivore and herbivore carcasses in a temperate forest ecosystem. Due to potentially increased disease transmission risk associated with carnivore carrion, we predicted that carnivore carcasses would be visited less and have a reduced visiting species composition compared to herbivore carcasses. Further, we expected red foxes to exhibit less consumption behavior of conspecific carcasses. We placed 22 red fox and 22 herbivore carcasses in a temperate montane protected area in Central Europe, the Bavarian Forest National Park, and analyzed the visits of all species and the consumption behavior of red fox at carcasses to understand whether these depended on carcass type. We found no significant effects of carcass type on visitation rates, visiting species composition, or red fox behavior. Cannibalism of foxes was common and, when controlling for confounding factors, e.g., elevation, temperature, and scavenging seasonality, the predicted consumption rate of red fox at conspecifics was higher than at herbivore carrion. Foxes appeared to consume conspecifics earlier than herbivore carrion, but the difference was not significant. At the very least, our results show that carnivore carcasses and fox behavior, including cannibalism, could provide a pathway for disease transmission. We thereby provide a first impression of the patterns of vertebrate scavenger assemblages at different carcass types in a temperate ecosystem and show that red fox behavior might precipitate a disease transmission pathway. [ABSTRACT FROM AUTHOR]

Rabies is endemic in Europe and red foxes are the vector and reservoir of the rabies virus (RABV). Based on classification established in the early 1990s, four variants of the rabies virus have been distinguished in Europe. Rabies broke out in January 2021 in the Mazowieckie voivodeship in central north-eastern Poland. The virus spread rapidly, reaching the Świętokrzyskie voivodeship in the central southern part and the Lubelskie voivodeship in the eastern part in the next months. Nine rabies cases were reported in the Podkarpackie voivodeship in south-eastern Poland between 2021 and 2023, mainly in red foxes but also in dogs and wildcat. The aim of the study was the identification of RABV variants in wildlife and domestic animals in Poland between 2021 and 2023. The study involved 157 animal brains tested positive for rabies using a fluorescent antibody test. From 10% w/v brain homogenates, RNA was isolated and full-length RABV genomes were high-throughput sequenced with an RABV-enriched approach. Complete genomes of RABV isolates were phylogenetically analysed and the variants were estimated. Molecular and phylogenetic studies revealed 147 (93.6%) of the RABV strains out of 157 which had rapidly spread in the wildlife of the Mazowieckie, Świętokrzyskie and Lubelskie voivodeships to be Central European strains. Nine RABVs (5.7%) detected in foxes, a wildcat and a dog in the Podkarpackie voivodeship were identified as North-Eastern European. A vaccine-induced rabies case was detected in a red fox in the Lubelskie voivodeship in May 2023. Central European and North-Eastern European RABVs were circulating in Poland between 2021 and 2023. [ABSTRACT FROM AUTHOR]

The aim of the study was to determine the species composition of the intestinal parasite fauna of foxes from the Pomerania region, with a particular emphasis on helminth species considered dangerous to humans, and to determine their prevalence and intensity of infection. In total, 165 digestive systems from foxes inhabiting the Pomeranian region were examined. The prevalence of intestinal parasites among the studied foxes was 61.8%. Our findings confirm that foxes in Pomerania carry various parasites, some of which pose a direct threat to human health. As such, constant monitoring of their infestation is essential. Particular attention should be paid to parasite species with potential for transmission to humans, such as Echinococcus multilocularis, Alaria alata and Toxocara canis, whose respective prevalence was found to be 10.9%, 17.6% and 28.5%. [ABSTRACT FROM AUTHOR]

This paper uses a selection of foxhunting stories to explore the way in which anthropomorphized foxes were used to communicate pro-hunting messages to their mostly young readers. These justifications also appeared in nonfictional foxhunting sporting literature. Indeed, this literature included some of the same incredible anecdotes that also appeared in the work of nature writers at the turn of the twentieth century such as Charles G.D. Roberts and Ernest Thompson Seton working in a new sympathetic genre called the “animal story.” These writers found themselves under attack because naturalists like John Burroughs, and hunters, including President Theodore Roosevelt, found their stories to be inaccurate and anthropocentric. Roosevelt dubbed them the “nature fakers.” Foxhunting “histories” employed imaginative material and much anthropomorphism but were not attacked for doing so. This tends to indicate that anthropomorphisms were more acceptable when they were facilitating animal exploitation than when they were used to promote the perspective of nonhuman animals. [ABSTRACT FROM AUTHOR]

Fire shapes animal communities by altering resource availability and species interactions, including between predators and prey. In Australia, there is particular concern that two highly damaging invasive predators, the feral cat (Felis catus) and European red fox (Vulpes vulpes), increase their activity in recently burnt areas and exert greater predation pressure on the native prey due to their increased exposure. We tested how prescribed fire occurrence and extent, along with fire history, vegetation, topography, and distance to anthropogenic features (towns and farms), affected the activity (detection frequency) of cats, foxes, and the native mammal community in south‐eastern Australia. We used camera traps to quantify mammal activity before and after a prescribed burn and statistically tested how the fire interacted with these habitat variables to affect mammal activity. We found little evidence that the prescribed fire influenced the activity of cats and foxes and no evidence of an effect on kangaroo or small mammal (<800 g) activity. Medium‐sized mammals (800–2000 g) were negatively associated with prescribed fire extent, suggesting that prescribed fire has a negative impact on these species in the short term. The lack of a clear activity increase from cats and foxes is likely a positive outcome from a fire management perspective. However, we highlight that their response is likely dependent upon factors like fire size, severity, and prey availability. Future experiments should incorporate GPS‐trackers to record fine‐scale movements of cats and foxes in temperate ecosystems immediately before and after prescribed fire to best inform management within protected areas. [ABSTRACT FROM AUTHOR]

The Morgado Superior Rock Shelter is located near the city of Tomar, in Central Portugal. This site presents important funerary contexts that according to published information encompass the Neolithic, Chalcolithic and Bronze Age. Other non-funerary uses have not yet been characterised but were briefly suggested. A faunal sample of the 1988 and 2012 interventions is analysed from a zooarchaeological and taphonomical perspective, focusing on the leporid remains that largely dominate the assemblage. Clear indicators of a human origin are absent, while a configurational approach allows the description of exogenous and intrusive origins. The characteristics of the registered consumption taphonomical indicators are suggestive of scat and non-ingested accumulation by foxes. Furthermore, this study allows us to discuss some aspects of the formation of the stratigraphy of this important cave necropolis, while also serving as an example of the expected results of foxes' action on leporid remains in an archaeological site. [ABSTRACT FROM AUTHOR]

Simple Summary: Avian influenza viruses are highly contagious respiratory viruses that severely impact bird populations, causing significant morbidity, mortality, and economic losses in the poultry industry worldwide. Particularly concerning are the Asian-origin H5 subtype highly pathogenic avian influenza viruses of clade 2.3.4.4b, which emerged in 2013 and have since spread across Asia, Europe, Africa, and America, leading to outbreaks in various poultry and animal species. The unique epidemiological and pathobiological characteristics specific to clade 2.3.4.4b viruses are discussed, emphasizing their distinct nature compared to other clades. Wild waterfowl, acting as reservoirs, frequently carry these viruses, posing threats not only to their populations but also to other wild bird species, including endangered ones. Furthermore, an increasing number of clade 2.3.4.4b virus infections in wild or domestic mammalian species raises significant concerns about potential spillover events to humans. This review highlights the diverse outcomes of HPAI infections in different hosts, ranging from asymptomatic cases to fatal infection, influenced by host and virus-related factors. Understanding these complexities is vital for developing effective strategies to mitigate the impact of AIVs, safeguard poultry production, protect wildlife, and prevent potential public health crises. Avian influenza viruses (AIVs) are highly contagious respiratory viruses of birds, leading to significant morbidity and mortality globally and causing substantial economic losses to the poultry industry and agriculture. Since their first isolation in 2013–2014, the Asian-origin H5 highly pathogenic avian influenza viruses (HPAI) of clade 2.3.4.4b have undergone unprecedented evolution and reassortment of internal gene segments. In just a few years, it supplanted other AIV clades, and now it is widespread in the wild migratory waterfowl, spreading to Asia, Europe, Africa, and the Americas. Wild waterfowl, the natural reservoir of LPAIVs and generally more resistant to the disease, also manifested high morbidity and mortality with HPAIV clade 2.3.4.4b. This clade also caused overt clinical signs and mass mortality in a variety of avian and mammalian species never reported before, such as raptors, seabirds, sealions, foxes, and others. Most notably, the recent outbreaks in dairy cattle were associated with the emergence of a few critical mutations related to mammalian adaptation, raising concerns about the possibility of jumping species and acquisition of sustained human-to-human transmission. The main clinical signs and anatomopathological findings associated with clade 2.3.4.4b virus infection in birds and non-human mammals are hereby summarized. [ABSTRACT FROM AUTHOR]

Red foxes (Vulpes vulpes) have interacted with humans during their common history. We used a contingent valuation method to assess the economic value of the management of this mesocarnivore's negative impacts. We carried out face-to-face interviews with 746 Greek residents, using a multiple-bounded discrete choice approach to estimate willingness to pay (WTP) for red fox management under three impact situations: attack domestic animals, reduce game, carry disease. About 51.9%, 33.0%, and 81.1% of the respondents stated a mean WTP of €34.1, €44.9, and €72.1 for each situation, respectively. The total annual amounts of €18.7 million, €15.7 million, and €61.7 million could be collected from the target population for red fox management when they attack domestic animals, reduce game, and carry disease, respectively. Attitudes and likeability toward foxes were negatively associated while knowledge about foxes and fear of them were positively associated with WTP. The younger, richer, less educated, rural, farmers, hunters, and pet owners were generally more willing to pay for red fox management across situations. Females were more willing to pay for managing predation on domestic animals, while males were more willing to pay for managing predation on game. Our findings showed that the Greek residents highly value the management of red foxes in all impact situations and would be valuable for further advising the management process. [ABSTRACT FROM AUTHOR]

Reconstructing biological invasions from historical sources can provide insights into how they occur but are difficult to do when invasions are poorly documented. Genetic signatures left by invaders can also offer insights into invasion routes, points of origin and general biology but often present conclusions that are contradictory to expectations. Here, we test the ability of continental‐wide microsatellite genotype data from 29 loci and 3122 samples to reconstruct the well‐documented invasion of red foxes Vulpes vulpes from the United Kingdom into Australia over 150 years ago, an invasion that has led to the extinction of many native species. Our analysis reveals several key signals of invasion evident in Australian foxes. They display lower levels of diversity than foxes sampled from the UK, exhibit clines in diversity from the point of introduction (south‐east Australia) to the edge of their range, and show strong evidence of allele surfing in westerly and north‐easterly directions. These characteristics are consistent with a single point of origin followed by rapid expansion in westerly and north‐easterly directions as suggested by historical records. We also find little genetic structure in foxes across Australia with only the vast Nullarbor Plains and Great Victoria Desert region presenting a detectable barrier to their dispersal. As such, no mainland region within the current range of foxes can be considered genetically isolated and therefore appropriate for localised eradication efforts. Overall, our analyses demonstrate the ability of comprehensive population genetic studies to reconstruct invasion histories even after more than 80 years since colonisation was stabilised. [ABSTRACT FROM AUTHOR]

In the Arctic tundra, predators face recurrent periods of food scarcity and often turn to ungulate carcasses as an alternative food source. As important and localized resource patches, carrion promotes co‐occurrence of different individuals, and its use by predators is likely to be affected by interspecific competition. We studied how interspecific competition and resource availability impact winter use of carrion by Arctic and red foxes in low Arctic Fennoscandia. We predicted that the presence of red foxes limits Arctic foxes' use of carrion, and that competition depends on the availability of other resources. We monitored Arctic and red fox presence at supp lied carrion using camera traps. From 2006 to 2021, between 16 and 20 cameras were active for 2 months in late winter (288 camera‐winters). Using a multi‐species dynamic occupancy model at a week‐to‐week scale, we evaluated the use of carrion by foxes while accounting for the presence of competitors, rodent availability, and supplemental feeding provided to Arctic foxes. Competition affected carrion use by increasing both species' probability to leave occupied carcasses between consecutive weeks. This increase was similar for the two species, suggesting symmetrical avoidance. Increased rodent abundance was associated with a higher probability of colonizing carrion sites for both species. For Arctic foxes, however, this increase was only observed at carcasses unoccupied by red foxes, showing greater avoidance when alternative preys are available. Supplementary feeding increased Arctic foxes' carrion use, regardless of red fox presence. Contrary to expectations, we did not find strong signs of asymmetric competition for carrion in winter, which suggests that interactions for resources at a short time scale are not necessarily aligned with interactions at the scale of the population. In addition, we found that competition for carcasses depends on the availability of other resources, suggesting that interactions between predators depend on the ecological context. [ABSTRACT FROM AUTHOR]

Generalist species, which exploit a wide range of food resources, are expected to be able to combine available resources as to attain their specific macronutrient ratio (percentage of caloric intake of protein, lipids and carbohydrates). Among mammalian predators, the red fox Vulpesvulpes is a widespread, opportunistic forager: its diet has been largely studied, outlining wide variation according to geographic and climatic factors. We aimed to check if, throughout the species' European range, diets vary widely in macronutrient composition or foxes can combine complementary foods to gain the same nutrient intake. First, we assessed fox's intake target in the framework of nutritional geometry. Secondly, we aimed to highlight the effects of unbalanced diets on fox density, which was assumed as a proxy for Darwinian fitness, as assessed in five areas of the western Italian Alps. Unexpectedly, the target macronutrient ratio of the fox (52.4% protein-, 38.7% lipid- and 8.9% carbohydrate energy) was consistent with that of hypercarnivores, such as wolves and felids, except for carbohydrate intakes in urban and rural habitats. The inverse relation between density and the deviation of observed macronutrient ratios from the intake target suggests that fox capability of surviving in a wide range of habitats may not be exempt from fitness costs and that nutrient availability should be regarded among the biotic factors affecting animal abundance and distribution. [ABSTRACT FROM AUTHOR]

Urban environments provide opportunities for some species but are inhospitable for others. However, those which thrive can be found at higher densities in human‐dominated landscapes than in more rural habitats. This highlights the importance of understanding species responses to human environments. It is not only important for the conservation of urban wildlife but also may provide fundamental insights into human‐wildlife coexistence globally.Here, we use citizen science data to predict the habitat suitability of Greater London for the West European hedgehog (Erinaceus europaeus), the red fox (Vulpes vulpes), the European badger (Meles meles) and the Eastern grey squirrel (Sciurus carolinensis).Results indicate that the four target species prefer different urban habitats, indicating different levels of urbanisation potential. Foxes and grey squirrels are able to thrive in inner urban areas, with the highest values of habitat suitability for these species found in central London, whereas for hedgehogs and badgers, the highest habitat suitability occurs in suburban and more rural habitats in outer London, respectively.These results highlight a gradient in urban tolerance, from squirrels and foxes to hedgehogs and badgers. This work also shows the importance of urban green spaces as habitats for wildlife. [ABSTRACT FROM AUTHOR]

Many different animal species are susceptible to SARS-CoV-2, including a few Canidae (domestic dog and raccoon dog). So far, only experimental evidence is available concerning SARS-CoV-2 infections in red foxes (Vulpes vulpes). This is the first report of SARS-CoV-2 RNA detection in a sample from a red fox. The RT-qPCR-positive fox was zoo-kept together with another fox and two bears in the Swiss Canton of Zurich. Combined material from a conjunctival and nasal swab collected for canine distemper virus diagnostics tested positive for SARS-CoV-2 RNA with Ct values of 36.9 (E gene assay) and 35.7 (RdRp gene assay). The sample was analysed for SARS-CoV-2 within a research project testing residual routine diagnostic samples from different animal species submitted between spring 2020 and December 2022 to improve knowledge on SARS-CoV-2 infections within different animal species and investigate their potential role in a One Health context. Within this project, 246 samples from 153 different animals from Swiss zoos and other wild animal species all tested SARS-CoV-2 RT-qPCR and/or serologically negative so far, except for the reported fox. The source of SARS-CoV-2 in the fox is unknown. The fox disappeared within the naturally structured enclosure, and the cadaver was not found. No further control measures were undertaken. [ABSTRACT FROM AUTHOR]