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Objective: Screening test results of the donors/ patients who applied to Blood Transfusion Center of Sakarya University Training and Research Hospital were retrospectively evaluated.Methods: A total of 13536 donors/patients who applied to our Blood Transfusion Center were included in the study. HBsAg, anti-HCV, and anti-HIV1/2 in blood samples were analyzed using ELISA methods. Syphilis antibodies were evaluated using VDRL test.Results: According to blood bank archives a total of 15665 donors applied for blood donation. During this time period, HBsAg positivity (1.5%), anti-HCV positivity (0.4%), VDRL positivity (0.3%), and anti-HIV 1/2 positivity (0.006%) were detected in respective percentages of donors. When the results were evaluated according to years of blood donation, we found that the rates of HBsAg positivity, and Anti-HCV positivity decreased significantly within last years, while rates of VDRL, and Anti-HIV positivities did not change significantly. Conclusion: The patients should receive blood transfusions with the lowest risk as much as possible. The most critical step to lower this risk down to zero is to provide safe, and permanent donors.