China began entering the 14th Five-Year Plan period in 2021. With the proposed carbon-neutral long-term goal, the strategic position of clean energy is becoming increasingly prominent. using biomass power generation is one of the main application ways. In order to study the mixed combustion process of coal and biomass, this paper takes 300MW lignite boiler as the research object and uses Fluent software to explore the influence of biomass types and the location of biomass nozzle on the mixed combustion, and obtains the corresponding temperature field, flue gas component field and the distribution of pollutant NO. The results showed that when the biomass particles were sprayed into a fixed position with a certain proportion, the NO emission of wheat, corn and cotton straw was reduced, and the effect of wheat straw was the most obvious. When a certain proportion of wheat straw and coal are co-fired, the higher the biomass nozzle position, the higher the peak temperature in the main combustion zone, and the better the emission reduction effect of NO.