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Abstract: The information provided by pollen counts cannot be ignored by allergists and allergic individuals, but the divergence sometimes observed with clinical observations has led the réseau national de la surveillance aérobiologique (RNSA) to initiate the monitoring network of allergens by immunosampling (Monalisa) project. Moreover, some recent papers has shown the influence of pollution in the region and even of the year on the content in pollen allergens, particularly that of birch pollen. Thus, the goal of the Monalisa project was to set up a measuring system based on an immunological method that would result in validation of this new way of measuring airborne pollen counts. This European Life Environment project includes nine partners: Bertin Technologies (France), RNSA (France), NPARU (England), UCO (Spain), UTU (Finland), UAM (Poland), UEVORA (Portugal), AIA (Italy), and Meteoswiss (Switzerland). The results obtained during the 2006 season and then in the 2007 campaign, after modifications made in the sampler, were not very good. The principal results concern the measurement of birch and olive allergens, captured essentially in the prepollination period and sometimes in the postpollination period. Detection of these allergens may be due either to their presence on submicroscopic particles emitted independently of pollination, these allergens cross-reacting with birch and olive allergens. The difficulties encountered in this study lead us to say that pollen counts, phenological observations and clinical data are still the best way to assess the effects of airborne pollen. [Copyright &y& Elsevier]

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