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Your search keyword '"Bruyas JF"' showing total 2 results
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2 results on '"Bruyas JF"'

1. Herpes virus équin-1 (EHV1) et transfert d’embryon : y-a-t-il des risques sanitaires ?

Author

Hebia, I, Duchamp, Guy, Destrumelle, S, Vautherot, Jean-François, Zientara, Stephan, Fiéni, F, Bruyas, JF, Inconnu, Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Virologie, and École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence Française de Sécurité Sanitaire des Aliments (AFSSA)

Subjects
[SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2006

2. [The bovine zona pellucida: differences in macromolecular composition between oocytes, pre-treated with A-23187 or not, and embryos].

Author

Bercegeay S, Allaire F, Jean M, L'Hermite A, Bruyas JF, Renard N, Tainturier D, and Barrière P

Subjects
Animals, Cattle embryology, Electrophoresis, Polyacrylamide Gel, Female, Molecular Weight, Oocytes drug effects, Zona Pellucida drug effects, Zona Pellucida Glycoproteins, Blastocyst chemistry, Calcimycin pharmacology, Cattle metabolism, Egg Proteins analysis, Fetal Proteins analysis, Membrane Glycoproteins analysis, Oocytes chemistry, Receptors, Cell Surface, Zona Pellucida chemistry
Abstract

The SDS-PAGE method was used to determine the composition of isolated bovine zona pellucida (ZP). This egg extracellular coat appears to be characterized by 3 major glycoproteins (ZP1, ZP2, ZP3), with apparent molecular weight (MW) of 80-70 kD, 66-63 kD and 60 kD, respectively, as revealed by 1-dimensional SDS-PAGE. After 2-dimensional SDS-PAGE, the zona pellucida electrophoretic pattern indicates a fourth glycoprotein, thus called ZP4. SDS-PAGE analysis of ZP isolated from oocytes and embryos after transit through the genital tract of A23187 pretreated oocytes allowed the description of modifications in glycoproteinic composition.

Published
1993

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