1. [Detection of processed animal protein: European experience and perspectives].
- Author
-
Plouvier BM, Baeten V, Maudoux JP, Vanopdenbosch E, Berkvens D, Degand G, and Saegerman C
- Subjects
- Animal Feed analysis, Animals, Chromatography veterinary, Europe, Food Handling standards, Immunologic Techniques veterinary, Microscopy methods, Microscopy standards, Microscopy veterinary, Polymerase Chain Reaction veterinary, Sensitivity and Specificity, Specimen Handling standards, Specimen Handling veterinary, Spectroscopy, Near-Infrared veterinary, Animal Feed standards, Dietary Proteins analysis, Prion Diseases prevention & control
- Abstract
European Commission Regulation (EC) No. 152/2009 imposes optical microscopy as the reference method for official controls to detect traces of animal protein in animal feed. Since 1 July 2004, the one-solvent technique has been the only authorised variant of optical microscopy. Its detection limit is 0.1% of meat-and-bone meal. Other techniques--using molecular biology (polymerase chain reaction, immunology), microscopy or near-infrared imaging--have been developed in the past ten years to supplement the official method, which has certain limitations. This paper compares and discusses the different techniques, highlighting the strengths of each technique in order to propose a feasible control scheme to improve the sensitivity and specificity of the technique for the detection of processed animal protein in livestock feed.
- Published
- 2012