Search

Introduction: Endodontic infections are characterized by their microbial polymorphism with the presence of pathogenic agents such as bacteria. The aim of this study was to investigate the presence of yeast in the root canals of teeth with pulp necrotic with or without apical periodontitis and to study the sensibility of these at disinfection at sodium hypochlorite to 2.5 %., Methods: Root canal samples, taken with paper points before and after disinfection with sodium hypochlorite dosed at 2.5 % on 50 single rooted teeth in 38 patients, were seeded in petri dishes containing Sabouraud Chloramphenicol (SC) (Conda Laboratories, Madrid). These stains were incubated in an incubator at 37°C for 48hours. The presence of yeast was confirmed by white colonies on the entire circumference of the tip paper. The identification of species was done by macroscopic and microscopic examinations associated with Blastese test., Results: The yeast colonies were isolated from 7 teeth (14 %) out of 50. All positive samples were from teeth with an open necrotic pulp (P<0.001). The only species found was Candida albicans. Immediate disinfection with sodium hypochlorite at 2.5 % did not show a complete elimination of yeasts., Conclusion: The results of this study show that the canal of necrotic teeth with or without apical periodontitis may shelter yeasts even after immediate disinfection with sodium hypochlorite 2.5 %., (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)

Samples were taken under strictly anaerobic conditions from the root canals of 22 patients having a tooth in which necrotic pulp was associated with an inflammatory peri-apical lesion an optical periodontitis. In the majority of cases (85%), these lesions were chronic and the pulp chamber was closed. One hundred and two strains were isolated, 71.7% of the bacteria being obligate anaerobes and 49.8% being Gram-negative bacilli. Their ability to produce beta-lactamases was tested and 8.8% gave a positive reaction in a cefinase test. These cefinase positive strains were nevertheless susceptible to 3rd-generation cephalosporins (cefoxitin) and to amoxicillin-clavulanate, with the exception of M. Morganii.