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【Purpose】Urban green spaces mitigate carbon emissions, boost carbon sinks, and address environmental risks. Analyzing their types, carbon stocks, and key tree species' sequestration capacity aids in improving green space management. This advances the "Carbon Peak, Carbon Neutral" strategy, guiding greening industry development and robust ecosystem construction.【Method】A systematic survey was conducted at representative urban green space sites, establishing observation plots to study species composition and woody plant populations. The carbon sequestration capacity of major tree species was evaluated considering baseline carbon stock and a latitude correction parameter.【Result】The total carbon stock in Suqian's urban green spaces stands at 136,285.47 tons, with regional green spaces contributing the most, followed by parks and protective green spaces, while subsidiaries account for the least. Analysis reveals that the arbor layer is the primary carbon pool, surpassing soil, shrub, and herb layers. Notably, different arbor species exhibit significant variations in carbon sink capacity. There is an obvious difference in the carbon sink capacity of different tree species, and among the 30 major tree species in Suqian City, there is an obvious difference in the carbon sink capacity of different tree species, and there is an obvious difference in the carbon sink capacity of different tree species，Populus adenopoda, Populus × canadensis, Cedrus deodara, Triadica sebifera, Celtis julianae, Celtis sinensis, Liquidambar formosana, Malus spectabilis, Ligustrum lucidum, Acer buergerianum, Osmanthus fragrans, Metasequoia glyptostroboides, Ginkgo bilobaet al. Carbon sink capacity is weaker, and the carbon sink capacity of species will not be insignificantly related to the length of the time interval, but will be affected by the latitudinal zonal space。【Conclusion】 For urban green space management, trees are pivotal, dominating carbon sequestration potential compared to shrubs and herbs. Thus, studying carbon sequestration and oxygen release of tree communities is crucial. Additionally, attention should be given to tree configuration and species selection, prioritizing locally adapted, high-carbon sequestration trees while considering landscape aesthetics and biodiversity conservation, for efficient urban green space management.

The biological diversity contributes to many aspects of human well-being and ecosystem function, however, we have known very little about microbial diversity due to the limitations of appropriate methodology underneath it. The development of biotech have brought revolutionary progress in the study of microbial diversity in which Biolog required to pay a lot of attention due to its ability of reflecting the metabolic situation of living microbial communities and have used widely in the study of soil microbial communities. However, there are some controversies during its operation procedure and incubation process, handling large data during the analysis might have also caused trouble in the overall process. The approach based on uses of “absolute used”, “INDIRECT” function in Excel could greatly optimize the data analysis, and the increase of principle components in Principle Component Analysis (PCA) were able to extract more information from original data. Besides, the method that through “Taylor” and “logic” transformation for original data before PCA analysis could achieve data analysis optimization. This paper have presented the applications and optimization of Biolog EcoPlates in studies of functional diversity of microbial communities, presented its inherent biases and prospects, provided some reference for the applications and popularization of Biolog EcoPlates for microbial study and finally, the results imply improving the knowledge of biotech in study of soil microbial functional diversity.