1. [Use of cultured, virus-infected cells to study the biogenesis of polarity of epithelial cells].
- Author
-
Sabatini DD, Ivanov IE, Gottlieb TA, Compton T, Gonzalez A, Beaudry G, and Rindler MJ
- Subjects
- Animals, Biological Transport, Cell Compartmentation, Cell Differentiation, Cell Line, Cytoplasm metabolism, Cytoskeleton physiology, Dogs, Epithelium microbiology, Glycoproteins metabolism, Golgi Apparatus metabolism, Immunohistochemistry, Kidney cytology, Microscopy, Electron, Receptors, Cell Surface physiology, Viral Proteins metabolism, Virus Replication, Cell Membrane ultrastructure, Epithelial Cells
- Abstract
The major characteristic of the eucaryote cell is the presence of specialized organelles in which macromolecular components responsible for various subcellular functions are segregated. The membranes of these organelles serve not only as divisions between the various cytoplasmic compartments, but also provide scaffolding within which the macromolecular complexes of the organelle assemble and become functionally integrated. It is obvious that because of the degree of complexity resulting from the existence of numerous compartments and membrane systems, the development of a genetic programme in a eucaryote cell requires not only the transcription of specific genes and translation in the cytoplasma of the resultant messenger RNA, but also the activity of mechanisms which ensure that each polypeptide reaches the site of its function, which may be in the cytosol, in a membrane, or in the luminal cavity of an organelle. In the special case of membrane proteins, such mechanisms must result not only in the specific distribution of polypeptides newly synthesized in the various types of cell membrane, but also the arrangement of them required in the lipid bi-layer necessary for their normal function.
- Published
- 1988