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The appearance of bacterial resistance and pathogenic infections makes efforts to find new drugs as natural bacterial agents continue. One of them is through the utilization of marine organisms such as seaweed. Some studies show the content of seaweed bioactive compounds has potential in the pharmacological field. This study aims to determine the type of secondary metabolite compounds and antibacterial activity of ethanol extract of Ulva intestinalis. Seaweed extraction using the maceration method for 3 x 24 hours and antibacterial activities were tested by disc diffusion method. The results showed that there were 5 types of secondary metabolite compounds in the extract, namely alkaloids, flavonoids, glycosides, saponins, and steroids. The antibacterial activity test of seaweed extract against Propionibacterium acnes and Aeromonas hydrophila bacteria in the concentration range of 50% to 100% showed a moderate inhibition zone diameter