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A novel bamboo-wood box beam was introduced in this paper, which consisted of laminated bamboo lumber flanges and OSB webs. Four-point bending tests were conducted on composite beams to investigate the effects of shear span ratio and stiffeners on failure mode and strength. The results showed that the composite beams with shear span ratio less than two failed in web shear failure, but for the others, the beams failed in twist and delamination of OSB in flanges. The load carrying capacity of beams decreased with the increase of shear span ratio. However, the mechanical performance of beams can be improved moderately by the presence of stiffeners, and theultimate bearing capacity and initial stiffness was increased by 16.5% and 13.1% respectively.