1. Ultrafast Fluorescence Dynamics in Flurbiprofen–Amino Acid Dyads and in the Supramolecular Drug/Protein Complex
- Author
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Ignacio Vayá, M. Consuelo Jiménez, Miguel A. Miranda, Aninda Chatterjee, and Thomas Gustavsson
- Subjects
Drug/protein binding ,Fluorescence upconversion ,Flurbiprofen ,Human serum albumin ,Time-correlated single photon counting ,Time-resolved fluorescence ,Chemistry ,QD1-999 - Abstract
The interaction dynamics between the drug flurbiprofen (FBP) and human serum albumin (HSA) has been investigated by time-resolved fluorescence spectroscopy, combining femtosecond fluorescence upconversion and picosecond time-correlated single photon counting. In order to obtain additional information on the drug/ protein interaction, several covalently linked model dyads, composed of FBP and tryptophan or tyrosine, were also studied. For all systems, the main feature was a remarkable dynamic FBP fluorescence quenching, more prominent in the dyads than in the protein complex. All systems also displayed a clear stereoselectivity depending on the (S)- or (R)-form of FBP, that was strongly influenced by the conformational arrangement of the investigated chromophores.
- Published
- 2017
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