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Compared with other self-healing mechanisms, embedding vascular networks in cementitious matrix enables repairing wider cracks and performing multiple healing cycles. For vascular-based self-healing cementitious composites, additive manufacturing (AM) allows fabricating vascular structures with complex geometry. For Fused Filament Fabrication (FFF), printing direction has great influences on the properties of the 3D-printed vascular network. To timely release the healing agent when cracks occur, selecting the proper printing direction for the vascular network in self-healing concretes is necessary. In this work, two commercial printing filaments, i.e., polylactic acid (PLA) and polyvinyl alcohol (PVA), are used to create the vascular structures. The influence of printing direction on the dissolution of 3D-printed PVA structure is investigated. Besides, the flexural strength and water tightness of samples with PLA vascular printed in different printing directions are compared. After the 4-point bending tests, epoxy resin is manually injected into the vascular networks to seal the cracks. It is found that the strength recovery and the permeability of the cracked specimens are influenced by the printing direction due to the different crack closure after unloading.