Search

Your search keyword '"Sterry, Wolfram"' showing total 2 results
Start Over Author "Sterry, Wolfram" Search Limiters Full Text Language german
2 results on '"Sterry, Wolfram"'

1. Water-filtered infrared-A (wIRA) can act as a penetration enhancer for topically applied substances

Author

Sterry, Wolfram, Ackermann, Hanns, Hoffmann, Gerd, Schanzer, Sabine, Meyer, Lars, Grone, Diego, Otberg, Nina, and Lademann, Jürgen

Subjects
water-filtered infrared-A (wIRA), penetration, stratum corneum, skin barrier, penetration enhancer, dermatopharmacokinetics, dye, fluorescein, curcumin, tape stripping method, spectroscopy, laser scanning microscopy, transepidermal water loss (TEWL), hydration of the epidermis, corneometry, skin surface temperature, hydrophilic, lipophilic, occlusive dressing, Medicine
Abstract

Background: Water-filtered infrared-A (wIRA) irradiation has been shown to enhance penetration of clinically used topically applied substances in humans through investigation of functional effects of penetrated substances like vasoconstriction by cortisone. Aim of the study: Investigation of the influence of wIRA irradiation on the dermatopharmacokinetics of topically applied substances by use of optical methods, especially to localize penetrating substances, in a prospective randomised controlled study in humans. Methods: The penetration profiles of the hydrophilic dye fluorescein and the lipophilic dye curcumin in separate standard water-in-oil emulsions were determined on the inner forearm of test persons by tape stripping in combination with spectroscopic measurements. Additionally, the penetration was investigated in vivo by laser scanning microscopy. Transepidermal water loss, hydration of the epidermis, and surface temperature were determined. Three different procedures (modes A, B, C) were used in a randomised order on three separate days of investigation in each of 12 test persons. In mode A, the two dyes were applied on different skin areas without water-filtered infrared-A (wIRA) irradiation. In mode B, the skin surface was irradiated with wIRA over 30 min before application of the two dyes (Hydrosun® radiator type 501, 10 mm water cuvette, orange filter OG590, water-filtered spectrum: 590–1400 nm with dominant amount of wIRA). In mode C, the two dyes were applied and immediately afterwards the skin was irradiated with wIRA over 30 min. In all modes, tape stripping started 30 min after application of the formulations. Main variable of interest was the ratio of the amount of the dye in the deeper (second) 10% of the stratum corneum to the amount of the dye in the upper 10% of the stratum corneum. Results: The penetration profiles of the hydrophilic fluorescein showed in case of pretreatment or treatment with wIRA (modes B and C) an increased penetration depth compared to the non-irradiated skin (mode A): The ratio of the amount of the dye in the deeper (second) 10% of the stratum corneum to the amount of the dye in the upper 10% of the stratum corneum showed medians and interquartile ranges for mode A of 0.017 (0.007/0.050), for mode B of 0.084 (0.021/0.106), for mode C of 0.104 (0.069/0.192) (difference between modes: p=0.0112, significant; comparison mode A with mode C: p

Published
2008

2. Analyse von Wundheilungsprozessen mit Hilfe von In-vivo-Laser-Scan-Mikroskopie

Author

Lademann, Jürgen, Kramer, Axel, Meyer, Lars, Alborova, Alena, Sterry, Wolfram, and Lange-Asschenfeldt, Bernhard

Subjects
Saugblasentechnik, Wundheilung, quantitative Analyse, 610 Medical sciences, Medicine, In-vivo-Laser-Scan-Mikroskopie
Abstract

Die erfolgreiche Entwicklung der optischen Technologien in den letzten Jahren führte zu Analysesystemen, die speziell im Bereich der Dermatologie neue Möglichkeiten der Anwendung erschließen. Im vorliegenden Beitrag wird über den Einsatz eines In-vivo-Laser-Scan-Mikroskops zur Charakterisierung von Wundheilungsprozessen berichtet. Im Gegensatz zum Goldstandard – den Messungen des transepidermalen Wasserverlusts (TEWL) – hat diese optische Methode den Vorteil, dass sie nicht durch Störfaktoren wie Wundflüssigkeit und topisch applizierte Substanzen beeinflusst wird. In der vorliegenden Studie wurden definierte oberflächliche Wunden mit Hilfe der Saugblasentechnik erzeugt und deren Abheilungsprozess mit Hilfe der In-vivo-Laser-Scan-Mikroskopie (LSM) untersucht. Es zeigte sich, dass die LSM-Technik eine objektive Bewertung des Wundheilungsprozesses ermöglicht. Durch die Einführung von charakteristischen Stadien der Wundheilung ist es sogar möglich, eine quantitative Bewertung des Wundheilungsprozesses vorzunehmen. Im Rahmen der Untersuchungen konnte u.a. nachgewiesen werden, dass der Wundheilungsprozess nicht nur von den Wundrändern her erfolgt. Auch um die Haarfollikel bilden sich Inseln von Korneozyten, die allmählich miteinander verschmelzen und zu einer ersten Bedeckung der Wundoberfläche mit einer geschlossenen Zellschicht von Korneozyten führen., GMS Krankenhaushygiene Interdisziplinär; 4(2):Doc06; ISSN 1863-5245

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results