1. Investigating the properties and antibacterial, antioxidant, and cytotoxicity activity of postbiotics derived from Lacticaseibacillus casei on various gastrointestinal pathogens in vitro and in food models
- Author
-
Asadi, Zahra, Abbasi, Amin, Ghaemi, Ali, Montazeri, Effat Abbasi, and Akrami, Sousan
- Subjects
cell-free supernatant probiotic ,lacticaseibacillus casei ,antibacterial capacity ,gas chromatography/mass spectrometry ,Medicine ,Public aspects of medicine ,RA1-1270 ,Microbiology ,QR1-502 - Abstract
Background: Postbiotics comprise soluble compounds freed from the structure of destroyed bacteria or created by living bacteria. Such byproducts provide the host with enhanced biological function as well as specific physiological consequences. This research aims to examine the characteristics and possible health advantages of -derived postbiotics. Methods: The antibacterial effects of postbiotics derived from were examined in vitro against various infectious gastrointestinal agents, as well as pasteurized milk and minced beef. Postbiotic activity potential was evaluated using disc-diffusion agar, minimum inhibitory concentration, minimum bactericidal concentration, and well-diffusion agar methods. Postbiotics were tested for antioxidant activity against zinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals. Additionally, the total phenolic and flavonoid content of the postbiotics was determined. The colorimetric MTT was used to investigate the potential cytotoxicity of postbiotics. The chemical makeup of the postbiotics was also determined using gas chromatography/mass spectrometry. Results: The antibacterial capacity was mostly related to pyrrolo[1,2-a] pyrazine-1,4-dione, benzoic acid, and laurostearic acid. Gram-positive microbes were more influenced by microbial byproducts in vitro than Gram-negative bacteria (P
- Published
- 2024
- Full Text
- View/download PDF