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In the present investigations heterologous lymphokine-containing fluids were examined in albino guinea pigs for their macrophage depletion activity. Cell-free ascites from Ehrlich ascites tumor and leukemia L 1210 of mice as well as supernatants of PHA-stimulated peripheral blood lymphocyte cultures were used. Ascites of Ehrlich's ascitic tumor and Leukemia L 1210 led to a significant depletion of macrophages. The application of supernatants of PHA-stimulated human lymphocyte cultures resulted in a dose-dependent reduction of peritoneal macrophages. On the other hand, a stimulation of the number of peritoneal macrophages by control supernatants of non-stimulated lymphocyte cultures was observed. The findings indicate the existence of two lymphokines having opposite effects.

The antiphlogistic effect of the benzopyrone drug "Venalot" could be proven in the model of the exsudative immune-peritonitis in the rat, caused by bovine serum-albumin. Under the action of the drug the exudate and the amount of proteins in the peritoneal fluid were reduced; an important reduction of the adhesions occurred, too. The differences between the mean values and observations in the control and in the treated group were statistically significant (P less than 0.001).