1. Superaufgelöste Mikroskopie: Pilze unter Beobachtung
- Author
-
Christian Stigloher, Ulrich Terpitz, Sebastian Sputh, and Sabine Panzer
- Subjects
Fluorescence-lifetime imaging microscopy ,Chemistry ,Pharmacology toxicology ,fungi ,Fusarium fujikuroi ,Structured illumination microscopy ,macromolecular substances ,Filamentous fungus ,law.invention ,law ,ddc:570 ,Microscopy ,Biophysics ,Electron microscope ,Molecular Biology ,Biotechnology - Abstract
The diffraction limit of light confines fluorescence imaging of subcellular structures in fungi. Different super-resolution methods are available for the analysis of fungi that we briefly discuss. We exploit the filamentous fungus Fusarium fujikuroi expressing a YFP-labeled membrane protein showing the benefit of correlative light- and electron microscopy (CLEM), that combines structured illumination microscopy (SIM) and scanning election microscopy (SEM).
- Published
- 2021