1. [Plasma-membrane fluidity studies of murine neuroblastoma and malignant melanoma cells under irradiation].
- Author
-
Busse E, Zimmer G, and Kornhuber B
- Subjects
- Animals, Buthionine Sulfoximine, Cell Cycle drug effects, Cell Cycle radiation effects, Cell Survival drug effects, Cell Survival radiation effects, Dose-Response Relationship, Radiation, Glutathione drug effects, Glutathione radiation effects, Melanoma, Experimental ultrastructure, Membrane Fluidity drug effects, Methionine Sulfoximine analogs & derivatives, Methionine Sulfoximine pharmacology, Mice, Neuroblastoma ultrastructure, Radiation Tolerance drug effects, Radiation-Protective Agents pharmacology, Radiation-Sensitizing Agents pharmacology, Tamoxifen pharmacology, Tumor Cells, Cultured drug effects, Tumor Cells, Cultured radiation effects, Melanoma, Experimental radiotherapy, Membrane Fluidity radiation effects, Neuroblastoma radiotherapy
- Abstract
Murine neuroblastoma (C-1300 NMB) and malignant melanoma (B16) cells were radiated in presence of radiopharmaceutics. Sensibilization was carried out with BSO and protection with TMX. Changes in fluidity of the plasma membrane, in cellular GSH contents and cell cycle were observed. After radiation fluidity of the plasma membrane is increased, whereas intracellular GSH decreased. These changes were intensified by BSO and reduced by TMX. Fluidity of the plasma membrane correlates with intracellular GSH and also with cell cycle. It is suggested that changes in plasma membrane fluidity can be used as an additional parameter for the determination of sensitivity towards radiation.
- Published
- 1992