Search

Copyright of Zentralblatt fuer Arbeitsmedizin, Arbeitsschutz und Ergonomie is the property of Springer Nature and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Laboratory Medicine / Laboratoriums Medizin is the property of De Gruyter and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Analysis of human thymus steroids by liquid-gel chromatography and gas chromatography-mass spectrometry revealed in dependence of age 4-pregnene-3,20-dione, 11 beta-hydroxy-4-pregnene-3,20-dione, 7 beta-hydroxycholesterol, and 7-ketocholesterol. 11 beta-Hydroxy-pregnene-3,20-dione should be the inhibitor of immune maturation.

Thin layer chromatography as extraction method for histological sections. A combined microanalytical and histochemical procedure. A combined histochemical and chromatographical procedure is described to identify tissue components such as lipids, free amino acids, carbohydrates and steroids in cryosections. Fixed or unfixed sections are extracted by solvents of decreasing or increasing polarity. The extracts are then analysed by chromatographical techniques known from analysis of tissue homogenates. The extracted sections can be controlled by histochemical staining. Our technique is demonstrated in detail by analysis of lipids in bovine seminal vesicle epithelium, by analysis of free-amino acids in rat brain and pituitary and by analysis of steroids in male rat adrenal gland.

The method described permits an exact and rapid determination of aldosterone in urine and plasma. The reliability of the method is based on the separation of aldosterone from contaminating steroids by thin-layer chromatography. The mobile phase used was: cyclohexane--ethyl acetate (20:80). The steroids were extracted by dichloromethane. Plasma was extracted directly, and urine after hydrolysis with sulfuric acid (pH = 1). Recovery before radioimmunological analysis of aldosterone was 54.8 +/- 7.2 (S.D.)%(N = 40) for urine samples, and 39.1 +/- 4.4% (n = 60) for plasma samples. The coefficient of variation for multiple determinations of aldosterone was for urine 8.2% for low (n = 10) and 7.8% (n = 10). The sensitivity of the determination of aldosterone was for urine 0.04 microgram per 24-h volume (n = 10) and for plasma 4.4 ng per 100 ml (n = 10). The method avoids pitfalls due to the cross-reaction of anti-aldosterone serum with other materials.