1. Analyse von Wundheilungsprozessen mit Hilfe von In-vivo- Laser-Scan-Mikroskopie.
- Author
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Lademann, Jürgen, Kramer, Axel, Meyer, Lars, Alborova, Alena, Sterry, Wolfram, and Lange-Asschenfeldt, Bernhard
- Subjects
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DERMATOLOGY , *SKIN diseases , *QUANTITATIVE research , *IN vivo toxicity testing , *BLISTERS , *EXTRACELLULAR fluid , *WOUND healing , *HAIR follicles , *EPITHELIUM - Abstract
Recent developments of new optical technologies in the past years lead to modern analytical systems suitable for new applications in the field of dermatology. In our study we describe the value of in vivo laser scanning microscopy (LSM) for the characterization of wound healing. In contrast to the gold standard the measurements of the transepidermal water lost (TEWL) this optical method has the advantage not to be disturbed by any interstitial fluid or topically applied substances. Standardized superficial wounds were produced by the suction blister technique and the healing process of the wounds was investigated using LSM. The LSM method has been proven to be a suitable technique for an objective evaluation of the wound healing process. Quantitative analysis of the healing process was facilitated by introducing scales characterizing the exact healing phase of the investigated wounds. This study revealed that the onset of tissue repair is not rectricted to the edges of the cutaneous wound but is found at the same time around the area of the hair follicles. Small islands of corneocytes were forming around the hair follicles leading to fully covered wound surface consisting of a first layer of corneocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2009