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Aim of this study was to find out whether nitrosative stress (via peroxynitrite(ONOO-)) plays a role or even causes various neurological diseases in children. In literature there have been several examples of neurological diseases which seem to caused or at least influenced by nitrosative stress, such as Alzheimers disease, ALS, inflammations, multiple sclerosis etc. Therefore cerebro spinal fluid (CSF) of 40 children and 3 adults (=43 patients) with various neurological diseases and CSF of 18 children out of a controll group was tested. The 43 patients suffered from diseases which in one way or the other could have been influenced by increased production of peroxynitrite. To show the excess of peroxynitrite production, 3-nitrotyrosine (3-NT) was measured, since peroxynitrite itself is a very instable product. 3-NT can be seen as an indicator of nitrosative stress (and peroxynitrite), since it is a stable product out of the reaction of tyrosine und peroxynitrite. High performance liquid chromotography (HPLC) combined with electrochemical detection was used to determine the amount of 3-NT. It seemed to be the method of choice because using this method even very small amounts of 3-NT could be detected. Furthermore this method isnt that expensive compared to other methods such as mass-spectroscopy, which is an important factor in routine 3-NT-investigation. At first, several experiments were made to show that our used machine worked exact and linear, and to show the influences of various parameters such as pH of mobile phase. This was followed by the investigation of CSF of patients and controll-group. But only eight out of the 43 patients had increased levels of 3-NT. In addition one child out of the cotroll group showed increased rates. The average concentration of 3-NT in the "3-NT-positive" probes was 4.73nM. In the other probes no 3-NT could be determined, that means the concentration was 0 nM. Now theres the question why only in eight out of the 43 patients an increased level of 3-NT could be detected. Three out of these eight patients were adults suffering from multiple sclerosis. All of the detected increased rates werent as high they were expected to be, compared to literature. One reason could be that never before children had been tested for 3-NT, so there is no direct comparison in literature. Mayby no 3-NT is produced in childrens brains at all, so no 3-NT could be shown in the controll group. Compared to this, the average rate of 4.73 nM 3-NT in "positive" probes could be an excessive increased level. Furthermore only free, not protein-bound 3-NT was measured. All of the in literature described 3-NT-increases were found in CSF probes, which proteins had been hydolysated before. Therefore, protein-bound-3-NT was as well detected. Protein-bound 3-NT wasnt detected in this study, so of course the detected rates were lower. Another thing is that one cant tell for sure that the detected substance really is 3-NT or another substance which has the same retention time and coelutes with 3-NT. To make sure that it is indeed 3-NT,one could gather the eluted substance and investigates it mass spectroscopidly. Finally one can say that there are many open questions,which need further studys. The CSF probes of the examined children should once more be tested for 3-NT hydrolysatinproteins. The found substanz should furthermore be definitly be identified as 3- NT. Only then the results can really be compared to results in literature. Therefore this study shall only be looked upon as a beginning of investigation in nitrosative stress causing neurological diseases in children, which has to followed by many other studys. It should be rather be looked upon as establishing a new method showing investigation starts.