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Several types of pesticides that are often used are chlorpyrifos (organophosphate), carbofuran (carbamate), and cypermethrin (pyrethroid). Pesticides can kill pests but also can cause toxic effects on humans when exposed. One of the adverse effects of pesticide exposure is a disturbance in the peripheral nervous system. Age is an essential factor in peripheral nerve damage. Nerve injury is one of the most common injuries in children and adolescents and is estimated at 10% to 15% of all exceptional unit cases. However, the outcome of peripheral nerve injury is known better in subjects who sustain the injury at a younger age. This study aims to determine the subchronic exposure effect of chlorpyrifos, carbofuran, and cypermethrin to sciatic nerve histopathology in juvenile rats. This study used 30 male Wistar rats (Rattus norvegicus) which were divided into 5 groups, normal group (N), control group (K), chlorpyrifos group (P1), carbofuran group (P2) and cypermethrin group (P3). Subcutaneous injection of pesticides was carried out for 21 days. Rats were sacrificed using the cervical dislocation method, and the right sciatic nerve was taken for histopathological observation. Assessment of the degree of ischiatic nerve histopathological damage is using the Jensen et al., (2018) method. Data were analyzed using the Kruskal-Wallis test and continued with the Mann-Whitney test. This study showed a significantly increased degree of histopathological damage in the chlorpyrifos group, carbofuran group, and cypermethrin group compared to the control group and the normal group (p

Carbofuran is a pollutant compound derived from the pesticide Furadan 3GR which is widely used in agriculture. Various methods of carbofuran degradation have been carried out, one of which is the conventional electrochemical method. This study used an electrochemical peroxidation process to degrade Carbofuran in the pesticide Furadan 3GR. This study aims to determine the optimum conditions (time, Na2SO4 concentration, and volume addition of H2O2) for electrochemical peroxidation and to compare the effectiveness of electrochemical and electrochemical peroxidation methods by measuring the parameter of carbofuran COD reduction. The significance of the electrochemical peroxidation method and the conventional electrochemical method was compared as a preliminary test. The COD reduction of Carbofuran using traditional electrochemical methods and electrochemical peroxidation was 45.76% and 88.70%, respectively. Batch carbofuran electrochemical peroxidation process was accomplished to ascertain the optimum conditions under various operation times, the concentration of Na2SO4, and the additional volume of H2O2. The largest COD reduction of 93.78% was obtained at 10 minutes, 75 mM Na2SO4, and 2 mL H2O2. The UV-Vis spectrophotometric absorption of Carbofuran at a wavelength of 274 nm was significantly reduced from 1.377 to 0.131 at optimum conditions. The IR spectrum measurement results indicate a reduction in absorbance for the N-H group (3383 cm-1) and the C=O group (1643 cm-1) at optimum conditions. Overall, the electrochemical peroxidation process proved to be an appropriate technique for COD reduction of Carbofuran in Furadan 3GR pesticides.

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.

Hydrophilic interaction liquid chromatography (HILIC) equipped with an environmentally friendly capillary column was employed to investigate the retention behavior of carbofuran; a polar stationary phase was used as well. Several conditions were conducted to investigate the retention behavior of carbofuran, such as a comparison study TSKgel®Amide-80 with another polar column, a comparison study retention behavior of carbofuran on various wavelengths, the water content effect on HILIC mode, the effect of buffer concentration on HILIC mode, and the analytical performance of carbofuran. The results showed that TSKgel®Amide-80 exhibited a better performance than other polar stationary phases in carbofuran determination, and observations at wavelengths of 251 and 254 nm showed higher absorbance for carbofuran than others. In addition, the increase of water content and salt buffer concentration in the mobile phase led to a shorter retention time. The recovery of this method was 101 ± 10.1%, while the limit of detection and the limit of quantification were 0.66 ppm and 2.22 ppm, respectively. Consequently, TSKgel®Amide-80 offers a good performance in carbofuran determination, even with the application of 10 cm length column capillary liquid chromatography

An analysis on the chemical compounds of flowers and leaves of Chromolaena Odorata (L.) R.M. King and H. Robinson from Timor island using GCMS, was conducted. Total number of the compound from flowers which can be detected were 18 considered of alkanes, oxygenated hydrocarbon, fatty acid, hydrazine, and alkaloid along with two unidentified compound (1,2). Twenty seven compound detected from leaves were identified as alkanes, fatty acid, alkaloid, hydrazine together with 12 unidentified compound (3-14). Compounds were identified using NIST Library data. Flower and leaves Chromolaena Odorata contained allelopathy compounds such as palmitic aid, linoleic acid, and 2.6-dimetoxy phenol.