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Every plant has a different secondary metabolite content, influenced by the location and conditions where the plant grows. Preparations from the butterfly pea flower (Clitoria ternatea L.) extract form have poor bioavailability. One effort to overcome this problem is by formulating it into nanoherbs form. This research aims to determine the phytochemical content and synthesize nanoherbs ethanol extract of butterfly pea flowers. The butterfly pea flowers were extracted using the maceration method, and the resulting thick purplish blue extract was 145.254 grams. The thick extract was analyzed for secondary metabolite content using phytochemical screening, and it was found to contain alkaloids, flavonoids, saponins, triterpenoids, and tannins. Nanoherb synthesis uses the ionic gelation method with an alginate polymer and a CaCl2 cross-linking agent. The synthesis result is a purple nanoherbs colloid with optimal variations in the ratio (10:1). The synthesized nanoherbs were characterized using PSA and obtained particle size results from the optimal variation of 220.4 nm, polydispersity index 0.2550, and zeta potential of -22.5 mV.

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Colletotrichum gloeosporioides, a pathogen of anthracnose disease, can significantly reduce the quality of mango (Mangifera indica) fruits. Chitosan as an antifungal agent can reduce fungal growth on post-harvest agricultural products. In its development, chitosan has been widely improved through its transformation into nanochitosan and its formulation with metals. One of the metals that has a large affinity and can be formulated with chitosan is copper (Cu). This study aimed to compare and determine the optimal concentration of chitosan, nanocithosan, and nanochitosan-Cu in suppressing the growth of C. gloeosporioides that cause decay on mango fruits. The synthesis of nanochitosan and nanochitosan-Cu was carried out by the ionic gelation method, while the characterization was performed using particle size analyzer (PSA). The antifungal activity assay was conducted through the poisoning method by mixing 500, 750, and 1000 ppm (w/v) chitosan, nanochitosan and nanochitosan-Cu into the growth media of C. gloeosporioides. The results of PSA analysis showed that chitosan, nanochitosan, and nanochitosan-Cu had an average size of 606.5, 386.8 and 254.1 nm, respectively. The formulation of chitosan into nanochitosan and nanochitosan-Cu increased the fungal activity on C. gloeosporioides with the inhibition percentage of chitosan, nanochitosan, and nanochitosan-Cu at 750 ppm was 35%, 70%, and 100%, respectively.

Nanoparticles are used in drug delivery which can increase mass transfer so increase the absorption and effectiveness of the drug. Therefore, its prospect to improve antibacterial and antioxidants activities of betel leaves. The research aimed to preparation and characterization of betel leaf extract using ionic gelation technique. The formulation of nanoparticles from betel leaf extract with ionic gelation method using alginate and CaCl2 with a ratio of 2.5: 1. The characterization of the nanoparticles includes particle size analysis, zeta potential, particle morphology and determination of flavonoid content. Particle size analysis demonstrated that the betel leaf extract nanoparticles had a particle size of 243,03 ± 1,48 nm, zeta potential of -23,0 ± 0,35 mV and morphology of particle showed that a flat shape. The betle leaf exctract nanoparticle positively contained flavonoid with Rf 0.7 equivalent to quercetin. The betel leaf extract can be made nanoparticles with ionic gelation method using alginate and CaCl2.