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This study aimed to detect genetic diversity associated with litter size traits of the P21-activated kinases (PAK1) gene in Senduro and Boerawa goats using the PCR-RFLP method. The restriction enzyme used is MSP1 (C*CGG). A pair of primers used in this study, namely Forward ((5'-GCTCAGTGTTGTATTAGCAG-3') and Reverse (5'- CAAGATATAAAGCACAGCCG-3') product length 713 bp (access code ENSCHIG00000003071). The samples used in this study consisted of 80 samples of Senduro goats and 20 samples of Boerawa goats from UPT PT-HMT Singosari, Malang Regency. The results showed that the PAK1 exon 9 gene in Senduro goats and Boerawa goats UPT PT-HMT Singosari was uniform (monomorphic), it was because all samples had GG genotype and G allele frequency of 100%. In conclusion, the PAK1 exon 9 / MSP1 gene is monomorphic and does not associate with litter size in Senduro goats and Boerawa goats, so it cannot be used as a genetic marker.

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ABSTRACT. Galur kambing Senduro ditetapkan melalui Keputusan Menteri Pertanian Republik Indonesia Nomor 1055/Kpts/SR.120/10/2014. Kambing Senduro sebagai kekayaan sumber daya genetik ternak lokal Indonesia memiliki fenotip yang dapat diukur berdasarkan sifat kuantitatifnya. Penelitian pertama bertujuan untuk mengkaji fenotip kambing Senduro betina berdasarkan pada tinggi pundak, panjang badan, lingkar dada, bobot badan, dan panjang telinga. Penelitian kedua bertujuan menghubungkan sifat kuantitatif dengan umur ternak. Penelitian pertama menggunakan metode observasi, sedangkan penelitian kedua menggunakan metode korelasi. Data penelitian ditabulasi dan dianalisis secara deskriptif kuantitatif dan analisis korelasi dengan menggunakan Microsoft Excel 2010 dan SPSS 26.0. Pengamatan dilakukan pada 102 ekor kambing Senduro betina. Hasil penelitian menunjukkan bahwa kambing Senduro betina memiliki tinggi pundak 70-80 cm (55,88%), panjang badan 70-80 cm (53,92%), lingkar dada 80-90 cm (59,80%), bobot badan 50-60 kg (32,35%), dan panjang telinga 30-40 cm (69,61%). Panjang badan, lingkar dada, dan bobot badan memiliki korelasi positif yang sangat kuat dengan umur ternak. Penelitian ini merekomendasikan bahwa perlu adanya upaya pembaharuan data dan informasi terkait fenotip kambing Senduro yang berkaitan dengan sifat kuantitatif ternak yang mengacu pada Keputusan Menteri Pertanian Republik Indonesia Nomor 1055/Kpts/SR.120/10/2014. (Phenotype study of Senduro goats as wealth of Indonesian local livestock genetic resources) ABSTRAK. The Senduro goat breed was determined through the Decree of the Minister of Agriculture of the Republic of Indonesia Number 1055/Kpts/SR.120/10/2014. The Senduro goat as a wealth of genetic resources for local Indonesian livestock has a phenotype that can be measured based on its quantitative characteristics. The first study aimed to examine the phenotype of female Senduro goats based on shoulder height, body length, chest circumference, body weight, and ear length. The second study aimed to evaluate quantitative characteristics with the age of Senduro goat. The first research uses the observation method, while the second research uses the correlation method. Observations were made on 102 female Senduro goats. The results showed that female Senduro goats had a shoulder height of 70-80 cm (55.88%), body length 70-80 cm (53.92%), chest circumference 80-90 cm (59.80%), body weight 50 -60 kg (32.35%), and ear length 30-40 cm (69.61%). Body length, chest circumference, and body weight have a very strong positive correlation with the age of Senduro goat. This study recommends that efforts to update data and information related to the phenotype of the Senduro goat related to the quantitative characteristics of Senduro goat are needed which refers to the Decree of the Minister of Agriculture of the Republic of Indonesia Number 1055/Kpts/SR.120/10/2014.