Objectives: to examine the prevalence and time trends in childhood overweight (including obesity) and obesity among children dwelling in Tuscany Region (Central Italy) in the period 2002-2016., Design: cross-sectional study at seven points (Tuscan Nutritional Surveillance System conducted in the years 2002, 2006, 2008, 2010, 2012, 2014, and 2016); height and weight of the subjects are directly measured by trained staff; body mass index was assessed by means of the International Obesity Task Force and of the World Health Organization cut-offs., Setting and Participants: representative sample of children dwelling in Tuscany Region (No. 10,155) aged between 8 and 9 years (5,258 boys and 4,897 girls)., Results: regarding the estimation of the absolute prevalence level of childhood overweight, a discrepancy was observed between the two criteria. In all surveys, more boys than girls were overweight (or obese). Trend analysis showed a significant decrease in the prevalence of overweight (including obesity) and obesity in Tuscan children in the period 2002-2016 (32.0% vs. 28.1%; p <0.001) and of 10.0% vs. 5.9% (p <0.001) for obesity., Conclusions: the present study is the first report from an Italian region showing a significant decrease in childhood obesity and overweight in the last 14 years. This reduction is probably a result of regional and local actions that were performed in many areas of society. However, efforts should be made to further reduce prevalence of childhood obesity and overweight.