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An experiment was conducted to investigate the effects of respondent revised progressive relaxation by Mitani Method (new PR) on left frontal (Fpl) electromyogram (EMG) and electroencephalogram (EEG) alpha waves. 20 subjects, 10 males, 10 females, mean age = 29, SD = 10.05, were divided into the experimental group and the control group. The experimental group received the respondent new PR. Intermittent two minutes vibrations and five minutes rests, namely DV 15-9-4.5. V vibration were given at shoulder and leg. The Fpl integrated EMG, integrated alpha wave and time of alpha wave appearance were measured by Mitani System. Results showed that only the experimental group showed significant integrated EMG reduction at Fpl. Alpha waves tended to appear more frequently. Integrated alpha wave, however, decreased significantly. Combined mean of integrated EMG and integrated alpha wave also decreased significantly. These results suggest the quick reduction of energy expenditure or entering into a deep relaxation of neuro-muscle circuit by the mechanical respondent New PR. As for operant New PR, experiment including such EEG data is not yet performed.

Contamination of 24 hr home bath with Legionella pneumophila is recently well recognized. Eradication of the water-bath contamination from L. pneumophila and other bacteria is an important matter to prevent the infection because the 24 hr-bathing facility is widely accepted in Japanese houses. Among the 16 bathing water samples we tested, Legionella pneumophia was isolated from 6 cases (37.5%) when the bathing water was not treated with disinfectants. Number of L. pneumophila increased up to 10(3) cfu/ml and total culturable bacterial counts reached to 10(5) cfu/ml within 5 days when the water was not treated. We selected 5 water baths among 6 positive cases to study the bactericidal effect of chlorine. As a result we concluded that the growth of L. pneumophila in 24 hr-water bath could be stopped by the 2 ppm chlorination program every day.