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A series of T-cell specific monoclonal antibodies (Leu-4; CD-3, OKT-4; CD-4, OKT8; CD-8) and a B-cell specific monoclonal antibody (Leu-12; CD-19) were used to detect the localization and intensity of lymphocytes in breast tissue. Frozen sections of 12 fibrocystic diseases, 11 fibroadenomas and 17 carcinomas of the breast were examined during this study. CD-3 and CD-8 positive lymphocytes predominated in and around the ducts of the fibrocystic disease and the fibroadenoma. Similarly, almost all of the reactive lymphocytes around the cancerous tissue also were CD-3 and CD-8 positive suppressor/cytotoxic cells.

Gypsum crystal growth on human dentin surface was investigated using a crystal growth agent composed of 4-20% ammonium sulfate and 5-60% acrylic acid aqueous admixtures. In the treatment with 4% ammonium sulfate which contains the concentration of acrylic acid from 5% to 60%, fine half-spherical aggregates of 25 microns in diameter of needle-like crystals were obtained from 35% acrylic acid and large ones from 30% acrylic acid. Using the crystal growth agent of 4% ammonium sulfate-35% acrylic acid aqueous admixture, the human dentin surface pretreated with sulfates of different cations was evaluated from crystal growth. All sulfates without 4% ZnSO4 formed half-spherical aggregates of needle-like crystals. Diameter of their half-spherical aggregates increased as follows; 4% Na2SO4 less than none-treatment less than saturated CaSO4.2H2O less than 4% K2SO4 less than 4% MgSO4 less than 4% ZnSO4. The half-spherical aggregates from sulfates of Na+ and K+ (alkali metal) grew finer than those from sulfates of Ca2+ and Mg2+ (alkali-earth metal) and Zn2+ (zinc) and their area on dentin surface increased more with sulfates of alkali metal than with those of alkali-earth.