1. [Characterization of anti-DNA antibody on IgG3-kappa type M-protein in a patient with rheumatoid arthritis].
- Author
-
Sato H, Terasawa N, Kaimori M, Takemori H, Otake T, and Sakurabayashi I
- Subjects
- Aged, Electrophoresis, Agar Gel, Humans, Immunoelectrophoresis, Male, Arthritis, Rheumatoid immunology, Autoantibodies blood, DNA immunology, Immunoglobulin G blood, Immunoglobulin kappa-Chains immunology, Myeloma Proteins immunology
- Abstract
A 66 year-old male patient with rheumatoid arthritis, was admitted due to bronchial pneumonia. Anti-nuclear antibody and anti-DNA antibody were both detected in the patient's serum and many LE-like cells were found in the pleural effusion. In addition, M-proteins(IgG-kappa type, IgM-kappa type) were detected in both serum and pleural effusion by immunofixation electrophoresis. Purified IgG fraction by gel filtration(Superdex 200HR) showed high titer of anti-nuclear antibody(x160) and anti-DNA(39.2 AU/ml) antibody. Furthermore, anti-DNA antibody was detected in the fraction III(fraction of M-protein) by ion-exchange chromatography. In conclusion, these results indicate that M-protein(IgG3-kappa type) contains anti-DNA antibody and it is suggests that M-protein induces the production of LE like cell in this case.
- Published
- 2003