For obtaining spectrum information and space distribution information of metameric targets at the same time, an AOTF-based imaging spectrometer was developed, which consists of a front imaging lens assembly, an AOTF filter imaging module, an AOTF driver, a plane array CCD, an image acquisition card and a PC. Under the control of the PC, a spectral image cube can be obtained by a fast wavelength scanning process, thereby the spectrum information of any point on the image is available. The developed imaging spectrometer, with a spectral range from 550-1,000 nm and a spectral resolution of 2.6 nm (@ 632 nm), and a less than 80 s switch time between any two wavelengths, has the characteristics of no mechanical moving part, low power dissipation and strong vibration resistance. The identification test of true and false roses and the information recovery experiment of altered physical evidence were carried out, The true and false roses were identified and the altered physical evidence was recovered, The experimental results showed that the imaging spectrometer has an excellent ability of identifying metameric targets, indicating a good application prospect.