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Chromosome 3C of Aegilops triuncialis was discovered with ability to be transferred preferentially in the case of its monosomic status in wheat background, whereas, those gametes without 3C would result in chromosome structural changes including deletions and translocations. In the present study, Triticum aestivum-Haynaldia villosa substitution line 4V(4D) developed in our laboratory, was crossed to T. aestivum c.v. Norin 26-Aegilops triuncialis 3C addition line, and the hybrids F1 were then backcrossed with common wheat in order to induce structural changes of 4V. Both chromosome C-banding and genomic in situ hybridization was applied to search such chromosome variations. In this case, total genomic DNA of Haynaldia villosa was labelled by Biotin-11-dUTP as probes and total genomic DNA of Chinese Spring as the block. Moreover, several chromosome changes within common wheat such as isochromosome 1BL.1BL(B39-2) and others were also revealed. The result indicated that two translocation lines T4VL.3AS(A47-10-3) and T4VS.4DL(A47-25-4), two telocentric chromosome lines A47-7-2(4VS) and A47-32-2(4VL), and two isochromosomes including 4VS.4VS(A47-23) and 4VL.4VL(A412-5-4) were identified from BC1F2 or BC1F3. This result indicated that gametocidal chromosome 3C of Aegilop triuncialis could effectively induce structural changes of both chromosome 4V of Haynaldia villosa and chromosomes of wheat.