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Start Over Descriptor "A-SYNUCLEIN" Language lithuanian
2 results on '"A-SYNUCLEIN"'

1. Parkinsono liga ir egzosomos: nuo patogenezės iki diagnozės ir gydymo.

Author

Mišeikaitė, A. and Vaitkienė, P.

Subjects
*NEUROLOGICAL disorders, *PARKINSON'S disease, *EXTRACELLULAR vesicles, *ALZHEIMER'S disease, *NERVE tissue
Abstract

Parkinson's disease (PD) is considered the second most common neurodegenerative disease after Alzheimer's disease, with a mean onset age of 55 years. Although the clinical symptoms, neuronal changes, and various molecular mechanisms involved in this neuropathological disorder have been studied for many years, the diagnosis and treatment of the disease remain challenging. Of great interest are exosomes, extracellular vesicles isolated from the cellular endosomal system. It has been investigated that most cells, including nerve cells, in the brain can secrete these 50-150 nm diameter vesicles which can affect protein activity and gene expression. Due to their internal content and ability to move in an intercellular environment, exosomes are considered to play an important role in the accumulation and transportation of various molecular markers, such as a-synuclein protein or microRNA (miRNA), and to take a part in inflammatory processes in nervous tissue. Since the content of the exosomes depends on the cell that secretes them and can change during the progression of the disease, exosome studies can help to understand the pathogenesis and control the course of the disease. There is growing evidence of the importance of exosomes for cell-to-cell communication, mediating the exchange of biological information and substances, and the ability to cross the blood-brain barrier. Due to their ability to cross the blood-brain barrier, exosomes may be promising for drug or molecular transportation and help in the development of more effective treatments for PD and other diseases of the nervous system. The article reviews the biogenesis of exosomes and their potential roles in the diagnosis, treatment, and long-term outcome of Parkinson's disease. [ABSTRACT FROM AUTHOR]

Published
2021
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2. Investigation of amyloid fibrils forming proteins

Author

Povilonienė, Simona, Laurinavičius, Valdas Stanislovas, Mildažienė, Vida, Sužiedėlis, Kęstutis, Šatkauskas, Saulius, Barauskas, Justas, Sereikaitė, Jolanta, Valinčius, Gintaras, and Vilnius University

Subjects
Abeta40 peptidas, A-Sinukleinas, Abeta40, mental disorders, A-Synuclein, macromolecular substances, Amiloidinės fibrilės, Nanoderivatives, Biochemistry, amyloid fibrils, a-Synuclein, nanoderivatives, Amyloid fibrils, nervous system diseases, Nanodariniai
Abstract

Self-assembly of biomolecules into beta-sheet structures can be applied in the creation of nano-materials with novel electrical, optical, catalytical, or/and mechanical characteristics. This work was directed towards the construction of nano-derivatives based on amyloid fibrils forming proteins (Abeta40 peptide, a-Synuclein (a-Syn), equine lysozyme (EL)). Such nanostructures can be used to produce nanoscale functional systems. Herein, different mutant and hybrid proteins, which were able to form fibrillar structures, were constructed and the properties of fibrils were investigated. Designed cysteine mutants of Abeta40 and a-Syn can be modified through thiol group of cysteine. Herein, for the first time, it was demonstrated that a-Syncys141 fibrils could be modified with biotin and gold nanoparticles with neutravidin molecules. Hybrid proteins of Abeta40 or a-Syn and other non-amyloid proteins were designed on purpose to obtain fibrils with active functional non-amyloid proteins. Under appropriate conditions, these proteins aggregated into beta-sheet structures. Hybrid protein of streptavidin and Abeta40 formed a net-like fibrillar structure, and streptavidin was active. For the first time it was described the production of recombinant EL in E. coli. Moreover, active EL can form fibrils, which are similar to the fibrils formed by native EL. Constructed novel hybrids and mutants that are able to form amyloid fibrils, can be applied for the creation of functionalized nanodevices... [to full text] Savitvarkės biomolekulės, gebančios formuoti beta-klosčių struktūras, gali būti pritaikomos nanomedžiagų su naujomis elektrinėmis, optinėmis, katalitinėmis ir/ar mechaninėmis savybėmis, kūrimui. Šiame darbe buvo siekiama kurti nanodarinius, grįstus amiloidines fibriles formuojančiais baltymais (Abeta40 peptidas, a-sinukleinas (a-Syn), kumelės pieno lizocimas (EL)), kurie būtų pagrindas nano dydžio funkcinių sistemų gamybai. Šiam tikslui buvo sukonstruoti mutantiniai ir hibridiniai baltymai bei tiriamos jų fibrilinių struktūrų savybės. Sukurti Abeta40 ir a-Syn cisteino mutantai, kurie gali būti modifikuojami per cisteino tiolinę grupę. Pirmą kartą buvo pademonstruotas a-Syncys141 baltymo fibrilių modifikavimas biotinu ir aukso nanodalelėmis su neutravidinu. Sukonstruoti hibridiniai baltymai, kurie sudaryti iš Abeta40 ar a-Syn bei GDH, streptavidino ir hidrofobino. Buvo tikimasi, kad tokie baltymai formuos fibrilines struktūras, o funkciniai baltymai bus aktyvūs. Esant atitinkamoms sąlygoms, šie baltymai agregavo suformuodami skirtingos morfologijos beta-klostines struktūras. Streptavidino ir Abeta40 hibridinis baltymas formavo fibrilinę struktūrą – tinklą, o streptavidinas buvo aktyvus. Šiame darbe pirmą kartą aprašoma rekombinantinio EL gamyba E. coli ląstelėse. Aktyvus EL gali formuoti panašias į natyvaus EL fibrilines struktūras. Sukonstruoti nauji hibridiniai ir mutantiniai baltymai, gebantys formuoti amiloidines fibriles, yra geras pagrindas, kuriant funkcionalizuotus... [toliau žr. visą tekstą]

Published
2011

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