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This paper presents a study which aims to validate a scale for assessing the impact of Web 2.0 in education. From a structural perspective, we evaluate the influence of various factors (Training, Attitude and Use of Web 2.0) on the Impact of Web 2.0 in education with a sample of 403 students from different degrees of the University of Huelva, applying a Likert scale constructed for this purpose. To analyse the psychometric properties of the scale, we conducted a Chronbach's Alpha reliability test and factor analysis. To validate the theoretical construct, we conducted an analysis according to structural equation modelling, which enabled us to confirm the influence of training, attitude and use on the Impact of Web 2.0, and to observe how the factors interrelate and influence each other. We conclude that the existence of a training plan is not enough in itself and that, rather, it is the approach taken to Web 2.0 and the use made of it that has more influence on its educational impact. [ABSTRACT FROM AUTHOR]