1. افزایش زمان پرتودهی سلولهای سرطانی رده 7-MCF و فعال شدن مسیرهای مرتبط با دیابت نوع ۱.
- Author
-
مجید کوثری, محمد رضا تقو, and اباذر روستازاده
- Subjects
- *
BREAST tumor diagnosis , *TYPE 1 diabetes , *BREAST tumors , *CELL physiology , *TREATMENT duration , *DESCRIPTIVE statistics , *IMMUNE system , *GENE expression , *CELL lines , *ISLANDS of Langerhans , *METABOLISM , *MICROARRAY technology , *RADIATION doses , *DATA analysis software , *ONTOLOGIES (Information retrieval) , *TUMOR necrosis factors , *DISEASE risk factors , *PHYSIOLOGICAL effects of radiation - Abstract
Introduction: Breast cancer is one of the most common cancers among women. Since microarray is a new method in cancer diagnosis, in the current study, the microarray library of MCF-7 breast cancer cells after irradiation was examined to predict the gene expression pattern and metabolic pathways related to it. Materials and Methods: Microarray data extracted from National Center for Biotechnology Information (NCBI) related to MCF-7 cells were analyzed by GEO2R and R software. Then the genes that had increased and decreased expression were extracted and gene ontology analysis was performed using DAVID and Enrichr databases. Results: In this study, after 48 hours of irradiation, 234 genes with Log2FC>1 increased expression and 146 genes with Log2FC<-1 decreased expression (Adjusted p value <0.05). KEGG analysis on genes with increased expression showed that these genes are related to type 1 diabetes (P=2.43E-04). The genes extracted in this pathway included HLA-DRB5, HLA-DRB4, CPE, TNF, HLA-DRB1 and HLA-DM (Adjusted p value <0.05). Also, the findings show that the genes involved in type 1 diabetes in the islets of Langerhans include CPE, MHC-II, and TNF-α. Conclusion: The findings of this study showed that the increase in irradiation time in breast cancer cells probably leads to the activation of pathways related to type 1 diabetes through CPE, MHC-II and TNF-α genes in the islets of Langerhans. [ABSTRACT FROM AUTHOR]
- Published
- 2024