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Background: Asthma is a common inflammatory disease of the airways which is characterized by variable and recurrent symptomes. The disease causes airway inflamtion and hyperresponsiveness, reversible airway obstruction and airway deformity, and accomponied with symptoms including shortness of breath, cough and wheezing in patients. Environmental and genetic factor ssuch as gene polymorphisms are involved in the development of asthma. Gene polymorphisms alter the function of genes, hence they involve in disease progression. Studies have shown genetic polymorphism in ADAM33 and TBX21 genes are associated with the risk of asthma. Materials and Methods: In present study, the polymorphism rs2787094 of ADAM33 and rs11650354 of TBX21 genes was assessed for the first time in Iranian patients with asthma and healthy individuals. For this purpose, 30 blood samples from patients with asthma and 30 samples from healthy donors were collected. Then their DNA was extracted, and subsequently ARMS-PCR technique was used, applying specific designed primers for normal and mutant allels for each polymorphism of candidate genes. Then the results were analysed. Results: The results indicated rs2787094 polymorphism in ADAM33 was significantly associated with the risk of asthma, while there was no significant relationship between rs11650354 in TBX21 and asthma risk. Conclusion: It appears that rs2787094 polymorphism in ADAM33 and different genotypes in this locus play a siginificant role in asthma risk in Iranian patients. [ABSTRACT FROM AUTHOR]