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In order to evaluate the effect of different amounts of nitrogen fertilizer, potassium sulfate, magnesium sulfate and zinc sulfate on biodiesel produced from safflower, a field experiment was carried out as completely randomized blocks design with three replications, at Research Farm of Shahrekord University in 2010. Treatments included nitrogen fertilizer at three levels (150, 200 and 300 kg/ha), potassium, magnesium and zinc sulfates at 150, 100 and 50 kg/ha, respectively, and control (no fertilizer application). By nourishing the safflower plants, the seed yield and biodiesel traits such as density, iodine value and saponification value were measured. The results showed that the seed yield under treatment of 300 kg/ha nitrogen (913 kg/ha) was greater than other treatments. Magnesium sulfate and potassium sulfate produced the highest oil percentage (32.84 and 32.5, respectively). The biodiesel production under utilization of potassium sulfate had greater density, iodine value and saponification value (867.25 kg/m3, 139.7 mg iodine per 100 g oil, and 190.6 mg sodium hydroxide per g oil, respectively) compared to other treatments. In general, it was concluded that application of micronutrient fertilizers (especially potassium sulfate) improves seed-oil and biodiesel characteristics of safflower.